Survival ofAlcaligenes xylosoxidans degrading 2,2-dichloropropionate and horizontal transfer of its halidohydrolase gene in a soil microcosm

Haloalkanoate Dehalogenase II (DehE) of a Rhizobium sp. — Molecular Analysis of the Gene and Formation of Carbon Monoxide from Trihaloacetate by the Enzyme

“…There are various reports of dehalogenases that act on both isomers of ClPpH and quantitative details of their reactivities against a range of other halogenated aliphatic acids have been presented [3,20,[22][23][24][25]. Some action against CI,AcH has been reported for some of these enzymes [3,20,22,251.…”

“…Some action against CI,AcH has been reported for some of these enzymes [3,20,22,251. The amino acid sequence reported here for DehE is similar to the reported sequences of two ClPpH dehalogenases of this type.…”

“…Comparison of DehE with Pseudomoizas ~~-2-haloacid dehalogenase [26], which also catalyses ClPpH dehalogenation with an inversion of the configuration at C2, showed 38 % sequence identity and 63 % similarity when conservative amino acid substitutions are taken into account. Comparison of DehE with DhlC (GenBank accession number X77610), a dehalogenase encoded on plasmid pFL40 of Alcaligenes xylosoxiduns and reported not to cause inversion of configuration at C, on dehalogenation of ClPpH [22], shows a significantly higher amino acid identity of 72% with 84 % similarity allowing for conservative substitutions. There is 35% overall identity between the three sequences, with three regions where the amino acid identity extends for seven or more consecutive residues.…”

Bioremediation of petroleum hydrocarbons through landfarming: Are simplicity and cost-effectiveness the only advantages?

“…Bioaugmentation should be implemented in contaminated sites where no indigenous petroleum hydrocarbon degrading bacteria exists, such as sites contaminated by high molecular weight polyaromatic hydrocarbons. The process of bioaugmentation should aim at 'seeding' the knowledge of degrading the pollutants to the indigenous bacteria (Brokamp & Schmidt 1991;Fulthorpe & Wyndham 1992;De Rore et al 1994;Top et al 1998Top et al , 1999. As the number of microorganisms tends to increase during biostimulation, the increase in the number of degrading bacteria can be used as potential bioindicators during bioremediation (Margesine et al 1999).…”

Effect of Dissemination of 2,4-Dichlorophenoxyacetic Acid (2,4-D) Degradation Plasmids on 2,4-D Degradation and on Bacterial Community Structure in Two Different Soil Horizons

“…Also, the high costs of mixing the soil layers to bring the bacteria in close proximity of the pollutant might be avoided. To date, a large number of studies have reported the occurrence of conjugative gene transfer between bacteria in soil (17,31); however, there is only little information about transfer of catabolic plasmids as a means of bioaugmentation (8,14,15,42,54,55). Research in our laboratory has shown before that this approach accelerates the degradation of biphenyl or 2,4-dichlorophenoxyacetic acid (2,4-D) in soil, although the introduced donor strains survived only between 3 and 14 days (14,54,55).…”