Survival Strategies of<i>Campylobacter jejuni</i>: Stress Responses, the Viable but Nonculturable State, and Biofilms

Svensson, Sarah L.; Frirdich, Emilisa; Gaynor, Erin C.

doi:10.1128/9781555815554.ch32

Cited by 20 publications

(15 citation statements)

References 165 publications

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“…This was also supported by difference in Gram staining of CF and RF cells. Whilst the rods and spiral cells were stained typically as Gramnegative bacteria, the coccoid cells were unable to retain as much counterstain (safranin or carbol fuchsin), indicating some changes in the cell wall structure [17,26].…”

Section: Properties Of Coccoid Formsmentioning

confidence: 99%

“…For example, CF formed under high temperature stress reveal much higher degradation of the cell wall as compared to CF induced under other conditions (described in more details in section "Temperature stress") [28] CF and VBNC Morphological changes to CF are coincident with the decrease in colony-forming unit (cfu) counts and were initially associated with the VBNC state [29,30]. The VBNC state is defined as "a state of dormancy where growth ceases on bacteriological media normally used for culture of the organism yet the bacteria retain vitality with minimal activity" [26].…”

Section: Properties Of Coccoid Formsmentioning

confidence: 99%

See 1 more Smart Citation

Putative mechanisms and biological role of coccoid form formation inCampylobacter jejuni

Ikeda¹,

Karlyshev²

2012

European Journal of Microbiology and Immunology

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Section: Properties Of Coccoid Formsmentioning

confidence: 99%

Section: Properties Of Coccoid Formsmentioning

confidence: 99%

Putative mechanisms and biological role of coccoid form formation inCampylobacter jejuni

Ikeda¹,

Karlyshev²

2012

European Journal of Microbiology and Immunology

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“…Astonishingly, this bacterium lacks the global stationary-phase regulator, the sigma factor RpoS, which induces expression of numerous proteins involved in different forms of stress response (18). Furthermore, C. jejuni also lacks the oxidative stress response regulatory elements SoxRS and OxyR (17) but expresses Dps, SodB, AhpC, and KatA in order to dispose of reactive oxygen species (ROS), and it also expresses GroES, GroEL, and DnaK in order to manage general and chemical stresses (19).…”

mentioning

confidence: 99%

Antimicrobial and Virulence-Modulating Effects of Clove Essential Oil on the Foodborne Pathogen Campylobacter jejuni

Kovács

Felső

Makszin

et al. 2016

Appl Environ Microbiol

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Our study investigated the antimicrobial action of clove (Syzygium aromaticum) essential oil (EO) on the zoonotic pathogen Campylobacter jejuni. After confirming the clove essential oil's general antibacterial effect, we analyzed the reference strain Campylobacter jejuni NCTC 11168. Phenotypic, proteomic, and transcriptomic methods were used to reveal changes in cell morphology and functions when exposed to sublethal concentrations of clove EO. The normally curved cells showed markedly straightened and shrunken morphology on the scanning electron micrographs as a result of stress. Although, oxidative stress, as a generally accepted response to essential oils, was also present, the dominance of a general stress response was demonstrated by reverse transcription-PCR (RT-PCR). The results of RT-PCR and two-dimensional (2D) PAGE revealed that clove oil perturbs the expression of virulence-associated genes taking part in the synthesis of flagella, PEB1, PEB4, lipopolysaccharide (LPS), and serine protease. Loss of motility was also detected by a phenotypic test. Bioautographic analysis revealed that besides its major component, eugenol, at least four other spots of clove EO possessed bactericidal activity against C. jejuni. Our findings show that clove EO has a marked antibacterial and potential virulence-modulating effect on C. jejuni. IMPORTANCEThis study demonstrates that the components of clove essential oil influence not only the expression of general stress genes but also the expression of virulence-associated genes. Based on this finding, alternative strategies can be worked on to control this important foodborne pathogen.

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“…However, the viability of Campylobacter spp. in water systems is favoured by biofilms (Svensson et al, 2008) and by free-living amoebae harboring bacteria intracellularly (Snelling et al, 2005). The presence of protozoan grazers such as Acanthamoeba spp.…”

Section: Persistencementioning

confidence: 99%

Members of the family Campylobacteraceae: Campylobacter jejuni, Campylobacter coli

Pitkänen¹,

Hänninen²

2019

Water and Sanitation for the 21st Century: Health and Microbiological Aspects of Excreta and Wastewater Management (Global Wate

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Survival Strategies ofCampylobacter jejuni: Stress Responses, the Viable but Nonculturable State, and Biofilms

Cited by 20 publications

References 165 publications

Putative mechanisms and biological role of coccoid form formation inCampylobacter jejuni

Putative mechanisms and biological role of coccoid form formation inCampylobacter jejuni

Antimicrobial and Virulence-Modulating Effects of Clove Essential Oil on the Foodborne Pathogen Campylobacter jejuni

Members of the family Campylobacteraceae: Campylobacter jejuni, Campylobacter coli

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