Survival to different acid challenges and outer membrane protein profiles of pathogenic Escherichia coli strains isolated from pozol, a Mexican typical maize fermented food

Sainz, Teresita; Pérez, Julia; Villaseca, Jorge M.; Hernández, Ulises Reyes; Eslava, Carlos; Mendoza, Guillermo; Wacher, Carmen

doi:10.1016/j.ijfoodmicro.2005.04.017

Cited by 25 publications

(25 citation statements)

References 28 publications

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“…OmpW has previously been described as a colicin receptor (Pilsl et al, 1999), but its function is unknown. Accumulating data suggest OmpW may be a stress protein in E. coli, because it has been expressed in strains challenged with acid (Sainz et al, 2005) and antibiotics (Xu et al, 2006) and in iron-limiting environments (Alteri and Mobley, 2007). Further investigations are therefore required to determine a possible role of BtuB and OmpW during growth of E. coli under different growth conditions and its possible role in the attachment process.…”

Section: Resultsmentioning

confidence: 99%

“…The bacterial outer membrane which is composed of many localized proteins known as outer membrane proteins (OMPs) is a critical interface between the microorganism and its surrounding environment. Some OMPs are highly regulated in response to the physiological state of growth, as well as nutrient and environmental conditions surrounding the microorganism (Seyer et al, 2005;Otto et al, 2001;Lin et al, 2002;Sainz et al, 2005;Alteri and Mobley, 2007). These changes in OMP expression can play an important role in bacterial pathogenesis by enhancing the adaptability of the microorganism to various environments (Lin et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

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Expression and Putative Roles in Attachment of Outer Membrane Proteins ofEscherichia coliO157 from Planktonic and Sessile Culture

Rivas

Fegan

Dykes

2008

Foodborne Pathogens and Disease

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Many strains of Shiga toxigenic Escherichia coli (STEC), particularly the serotype O157:H7, are foodborne pathogens causing disease in many countries throughout the world. E. coli O157:H7 is able to attach and survive on various surfaces such as stainless steel (SS) found within the food processing environment. We examined the outer membrane protein (OMP) profiles of four E. coli O157 (three toxigenic O157:H7 and one nontoxigenic O157:HR) and one non-STEC strain (O1:H7), previously reported to have different abilities to attach to SS following growth in planktonic (nutrient broth) and sessile (nutrient agar) culture. The OMPs of the five E. coli strains grown in planktonic and sessile culture were extracted using N-lauroyl sarcosine and the OMP profiles were separated using two-dimensional (2D) gel electrophoresis. Qualitative and quantitative variations in the total number of OMPs expressed between planktonic and sessile cultures were found for all E. coli isolates tested. A number of differentially expressed protein spots were selected from 2D gels and were identified. FlgE was found to be expressed in planktonic culture but not sessile culture. MipA and OmpX had higher expression in sessile culture than planktonic culture, while expression of OmpA did not differ between E. coli strains or between the two modes of growth. Although differential expression of OMPs was found between isolates grown in planktonic and sessile culture, further investigations are required to determine a role of some of these identified proteins during growth of E. coli in planktonic and sessile culture and their influence during the attachment process.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Expression and Putative Roles in Attachment of Outer Membrane Proteins ofEscherichia coliO157 from Planktonic and Sessile Culture

Rivas

Fegan

Dykes

2008

Foodborne Pathogens and Disease

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“…These included TreC (trehalose-6-phosphate hydrolase, affected by osmotic stress [8]); OmpA, OmpW, AceF, and DppA (outer membrane proteins A and W, pyruvate dehydrogenase, and periplasmic dipeptide transport protein, respectively, affected by pH, starvation, and other stresses [24,31,38,41]); and SspA (stringent starvation protein A, a starvation, salt, and putative global stress response regulator [14]). Although a coherent pattern for the expression of these diverse stress-responsive proteins was hard to discern, we investigated several candidate stress response systems to see if they might play some part in the chromate preadaptation phenotype.…”

Section: Discussionmentioning

confidence: 99%

Effect of Chromate Stress on Escherichia coli K-12

et al. 2006

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The nature of the stress experienced by Escherichia coli K-12 exposed to chromate, and mechanisms that may enable cells to withstand this stress, were examined. Cells that had been preadapted by overnight growth in the presence of chromate were less stressed than nonadapted controls. Within 3 h of chromate exposure, the latter ceased growth and exhibited extreme filamentous morphology; by 5 h there was partial recovery with restoration of relatively normal cell morphology. In contrast, preadapted cells were less drastically affected in their morphology and growth. Cellular oxidative stress, as monitored by use of an H 2 O 2 -responsive fluorescent dye, was most severe in the nonadapted cells at 3 h postinoculation, lower in the partially recovered cells at 5 h postinoculation, and lower still in the preadapted cells. Chromate exposure depleted cellular levels of reduced glutathione and other free thiols to a greater extent in nonadapted than preadapted cells. In both cell types, the SOS response was activated, and levels of proteins such as SodB and CysK, which can counter oxidative stress, were increased. Some mutants missing antioxidant proteins (SodB, CysK, YieF, or KatE) were more sensitive to chromate. Thus, oxidative stress plays a major role in chromate toxicity in vivo, and cellular defense against this toxicity involves activation of antioxidant mechanisms. As bacterial chromate bioremediation is limited by the toxicity of chromate, minimizing oxidative stress during bacterial chromate reduction and bolstering the capacity of these organisms to deal with this stress will improve their effectiveness in chromate bioremediation.Chromate [Cr(VI)] is a widespread environmental pollutant, as it is a by-product of numerous industrial processes and nuclear weapons production (5). Because chromate is soluble, environmental contamination is difficult to contain. This solubility also promotes the active transport of chromate across biological membranes (7), and once internalized by cells, Cr(VI) exhibits a variety of toxic, mutagenic, and carcinogenic effects (34, 44). In contrast, most cells are impermeable to Cr(III), which is insoluble under typical environmental conditions (30); as measured by the Ames test, it is therefore some 1,000-fold less mutagenic than Cr(VI) (19). Thus, strategies for decontamination of environmental chromate focus on reducing it to Cr(III). Chemical methods for this are prohibitively expensive for large-scale environmental application and frequently have damaging consequences of their own (7), and so bacterial bioremediation is of considerable interest as an environmentally friendly and affordable solution to chromate pollution.Several bacteria, including Escherichia coli, Shewanella oneidensis, and numerous species of Pseudomonas and Bacillus, can reduce Cr(VI) to Cr(III) (17, 39); nonetheless, an effective bacterial system for in situ reduction has not yet been developed. One reason is that chromate is also toxic to the remediating bacteria (17). Our in vitro studies have strongly i...

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“…1B). The homologous protein is required for NaCl osmotic-resistance in Vibrio alginolyticus (21), Photobacterium damsela (20), and for acid-resistance in E. coli (18). Although its functional role in bacterial viability is unclear, this increase suggested that OmpW is an oxidation-responsible protein in E. coli.…”

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confidence: 99%

Proteomic Characterization of Enterohemorrhagic Escherichia coli O157:H7 in the Oxidation‐Induced Viable but Non‐Culturable State

Asakura

Panutdaporn

Kawamoto

et al. 2007

Microbiology and Immunology

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Enterohemorrhagic Escherichia coli (EHEC) O157 strain F2, a food isolate of an outbreak, is resistant to oxidative stress, but has increased stress-sensitivity after passage through mice. The stress-sensitive variant of F2 (designated MP37) has decreased culturability, but retains membrane integrity under stress conditions, indicating that the cells enter a viable but non-culturable (VBNC) state. Proteomic analyses revealed that MP37 in the VBNC state had decreased levels of some oxidation-responsive factors (AhpCF, AceF), but it markedly increased levels of outer membrane protein W (OmpW). Because F2 expressed higher levels of some ribosome-associated proteins (RaiA, S6, Bcp) than MP37, the effect of animal passage on the induction of the VBNC state in the EHEC O157 cells might be due to ribosomal activity.

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Survival to different acid challenges and outer membrane protein profiles of pathogenic Escherichia coli strains isolated from pozol, a Mexican typical maize fermented food

Cited by 25 publications

References 28 publications

Expression and Putative Roles in Attachment of Outer Membrane Proteins ofEscherichia coliO157 from Planktonic and Sessile Culture

Expression and Putative Roles in Attachment of Outer Membrane Proteins ofEscherichia coliO157 from Planktonic and Sessile Culture

Effect of Chromate Stress on Escherichia coli K-12

Proteomic Characterization of Enterohemorrhagic Escherichia coli O157:H7 in the Oxidation‐Induced Viable but Non‐Culturable State

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