Survival variability of controls and definition of imaging endpoints for longitudinal follow-up of pancreatic ductal adenocarcinoma in rats

Abstract

The early detection by the means of animal imaging of one or more signs indicating the onset of a critical step in the development of the disease (e.g., ascites or/and metastasis) allows the researcher to prevent the occurrence of animal pain, thereby ensuring better animal welfare. However, using a single standard control group in an effort to use fewer animals for a given model runs such a significant risk of false results that it mars the entire study. Although reducing the number of animals in a study remains the gold standard of our experimental practice, in this case it would come at the price of a loss of validity of the results.

<p>SLC5A1 promotes growth and proliferation of pancreatic carcinoma via glucose-dependent AMPK/mTOR signaling</p>

“…More ascites were observed in mice with wild type Panc2 cells, while mice implanted with SLC5A1-knockdowned Panc-2 cells due to the smaller tumor burden and progression (data not shown). A previous study has shown that ascites was a significant factor leading to poor survival of pancreatic cancer animals 44. Although we cannot fully address the reason of better survival in the knockdown group, it is possible that less ascites are associated with better prognosis of mice with SCL5A1-knockdowned Panc-2 cells.…”

Refinement in the European Union: A Systematic Review

“…A total of 384 references were identified by electronic search; 338 full-text studies were evaluated in accordance with the eligibility criteria and 290 were excluded. Finally, 48 studies [ 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 ] complied with all the established eligibility criteria and were included in the study ( Figure 1 ).…”