Survivin as a Prognostic Factor for Osteosarcoma Patients

Abstract

Purpose: Survivin is one of the apoptosis inhibitor genes and is rarely expressed in adult ­tissues. However, survivin expression has been detected in various human cancers and ­correlations have been recognized between the level of expression of this gene in tumors and prognosis. In this study, we investigated the correlations between survivin mRNA expres­sion in osteosarcoma tissues and clinicopathological parameters.Methods: There were 22 osteosarcoma patients in our hospital with paraffin-embedded ­tissues which could be extracted from biopsy specimens. We used the RT-PCR method after extracting total RNA and conducted a densitometric analysis to determine the ratio of survivin relative to h-GAPDH as an internal marker.Results: Expression of survivin mRNA was detected in all osteosarcoma samples. Patients with metastasis had high survivin mRNA levels in initial biopsy specimens (p<0.01). Moreover, there was a statistically significant difference in survivin mRNA expression between ­patients with and without metastasis (p<0.01).Conclusion: We concluded that high levels of survivin mRNA expression suggest poor prognosis for osteosarcoma patients.

Analyses of Novel Prognostic Factors in Neuroblastoma Patients

“…26) We have previously suggested that it is an important prognostic factor in NB (14 cases) and osteosarcoma patients (22 cases). 27,28) However, in the larger number of 22 NB cases in the present study we could not find any difference among the 22 NBs and 4 OTs, the early stages (stage I, II, IVS) and advanced stages (stage III, IV), and between surviving and dead patients (Fig. 2).…”

Immunohistochemical characteristics of cystic odontogenic lesions: a comparative study

“…Survivin is expressed in many various human malignancies including hepatocellular carcinoma, colorectal cancer, lung cancer, pancreatic and kidney cancers, and osteosarcoma (2)(3)(4)(5)(6)(7). Survivin plays an important role both in initiation and progression of these tumors (2)(3)(4)(5)(6)(7).…”

Expression and Function of Survivin in Canine Osteosarcoma

“…Small studies in human osteosarcoma have suggested that survivin may be useful in determining prognosis and degree of malignancy (27)(28)(29); however, definitive studies about the role of survivin in human osteosarcoma are lacking. Survivin expression is a negative prognostic factor in dogs with B-cell lymphoma (30), as has been shown in human B-cell lymphoma (31), and survivin expression has been identified in other canine neoplasms (30).…”