“…After an 8‐day culture in suspension, EBs were transferred on to gelatin‐coated plates and cultured for an additional 20 days. Subsequently, the differentiated cells were seeded at 1 × 10 4 cells/well on gelatin‐coated 96‐well culture plates and cultured with 0, 0.01, 0.1, 1, 10, or 100 µg/ml GCV for an additional 7 days, and cell viability was determined by WST‐8 assay using the Cell Count Reagent SF (Nacalai Tesque) . On the other hand, to assess cytotoxicity against undifferentiated hPSCs, cells were seeded at 2 × 10 4 cells/well on Matrigel‐coated 96‐well culture plate under nondifferentiating conditions and subsequently cultured with 0, 0.01, 0.1, 1, 10, or 100 µg/ml GCV for 7 days, followed by WST‐8 assay to determine cell viability.…”