Susceptibility and Activity of Glutathione<i>S</i>-Transferases in Nine Field Populations of<i>Panonychus citri</i>(Acari: Tetranychidae) to Pyridaben and Azocyclotin

Niu, Jinzhi; Li, Guoying; Dou, Wei; Wang, Jinjun

doi:10.1653/024.094.0227

Cited by 32 publications

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“…Resistance was the highest in the DG population and lower in the YN population for these three insecticides. It was reported that there was a positive correlation between GST activities and the toxicities of pyridaben against nine field populations of Panonychus citri from Chinese citrus orchards (Niu et al, 2011). In Aedes aegypti, an Epsilon GST, GSTE2, is very efficient at metabolizing DDT, and the expression of this enzyme is elevated in a DDT and pyrethroid-resistant population from Thailand (Lumjuan et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Purification and biochemical characterization of glutathione S‐transferases from four field populations of Bactrocera dorsalis (Hendel) (Diptera: Tephritidae)

Dou

Wang

et al. 2011

Arch Insect Biochem Physiol

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Glutathione S-transferases (GSTs) are a group of detoxification enzymes that catalyze the nucleophilic addition of glutathione to a wide variety of endogenous and xenobiotic compounds. In this study, GSTs were purified from four field populations of Bactrocera dorsalis with different insecticide susceptibilities by glutathione-agarose affinity chromatography. The populations were collected from Dongguan (DG) and Guangzhou (GZ) of the Guangdong Province, Haikou of the Hainan province (HN), and Kunming of the Yunnan province (YN), China. Differences in GST characteristics among the four populations were studied using purified enzyme samples through comparative SDS-PAGE, kinetic, and inhibition experiments. The specific activities of the purified enzymes were similar, but the purification yield of the GZ population (31.54%) was the lowest. SDS-PAGE analysis showed only one band at approximately 23 kDa for these four populations. Kinetic analyses showed that the affinities of the purified GSTs from the GZ and YN populations for 1-chloro-2.4-dinitrobenzene (CDNB) were much higher than those of GSTs from the other two populations, whereas the HN population had the highest catalytic capability in terms of V(max) value. The optimum temperature for CDNB conjugation was 37 °C and the optimum pH was 7.5 in all four populations. Inhibition kinetics showed that ethacrynic acid, diethyl maleate, tetraethylthiuram disulfide, curcumin, bromosulfalein, and β-cypermethrin had excellent inhibitory effects on GSTs in the four populations of B. dorsalis, but the low inhibitory effects of malathion and avermectin did not differ between populations. These results suggest that GSTs may have a role in detoxification of β-cypermethrin in B. dorsalis.

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Section: Discussionmentioning

confidence: 99%

Purification and biochemical characterization of glutathione S‐transferases from four field populations of Bactrocera dorsalis (Hendel) (Diptera: Tephritidae)

Dou

Wang

et al. 2011

Arch Insect Biochem Physiol

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“…Therefore, in vitro inhibition effect (based on I 50 ) of pyridaben on purified GSTs in different populations was determined. In addition, the I 50 s of the acaricide azocyclotin which could be good alternative acaricide to control pyridaben resistance (Niu et al 2011) and EA which is the specific inhibitors of GSTs Niu et al 2011) were also tested. The current result showed that azocyclotin presented the most excellent inhibitory effects against three populations compared with pyridaben and EA.…”

Section: Discussionmentioning

confidence: 99%

“…All the experiments were conducted with three to five days old adult females. The bioassay of pyridaben and azocyclotin against these three populations were determined by a slide-dip method (Niu et al 2011). The LC 50 estimates of pyridaben were 2.0, 5.3, and 14.9 mg l -1 for the BB, WZ, and ZX populations, respectively, while the LC 50 estimates of azocyclotin were 1,463, 104, and 734 mg l -1 for the BB, WZ, and ZX Populations, respectively (Niu et al 2011).…”

Section: Mitesmentioning

confidence: 99%

“…Moreover, GSTs-based resistance to insecticides was observed to be facilitated with the increase in the level of GSTs activity, while the responsible molecular genetic mechanisms for this upregulation of activity were gene amplification or overexpression (Li et al 2007). However, elevated levels of GSTs activity have also recently been shown to associate with spider mite resistance to acaricides, particularly in abamectin resistant populations of Tetranychus urticae Koch (Acari: Tetranychidae; Stumpf and Nauen 2002;Konanz and Nauen 2004), abamectin/fenpropathrin resistance populations of T. cinnabarinus Boisduval (Acari: Tetranychidae; He et al 2009), chlorpyrifos and lambda-cyhalothrin resistance populations of Panonychus ulmi Koch (Acari: Tetranychidae; Kumral et al 2009), pyridaben resistance populations of P. citri McGregor (Acari: Tetranychidae; Niu et al 2011).…”

Section: Introductionmentioning

confidence: 99%

“…Since the first acaricide resistance was reported in China in 1979, over the following decades citrus red mites have developed resistance to dicofol, pyrethroids, organotin miticide, hexythiazox, spirotetramat, amitraz, propargite, diafenthiuron, abamectin, mitochondrial electron transport inhibitor (METI) acaricides and spirodiclofen (Huang 1979;Ran et al 2009;Hu et al 2010), but the studies to identify the biochemical mechanisms were carried out just in a few cases Ran et al 2009;Niu et al 2011). Despite reliance on acaricides for control of P. citri, detoxification in these species is poorly understood.…”

Section: Introductionmentioning

confidence: 99%

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Purification and partial characterization of glutathione S-transferases from three field populations of Panonychus citri (Acari: Tetranychidae)

et al. 2011

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Glutathione S-transferases (GSTs) play central roles in phase II detoxification of both xenobiotics (drugs, insecticides, and herbicides) and endogenous compounds in almost all living organisms. In this study, we successfully purified the GSTs from the citrus red mite, Panonychus citri, by affinity chromatography on Glutathione Sepharose 4B and compared the biochemical characterizations of the purified GSTs from three field populations [beibei (BB), wanzhou (WZ), and zhongxian (ZX)]. SDS-PAGE revealed that the molecular weight of GSTs from three populations consisted of two subunits of 27.3 and 26.1 kDa. The specific activity of the purified GSTs from the WZ and ZX populations was increased 1.5- and 3.8-fold, respectively, compared with the BB population. Accordingly, the pyridaben susceptibility of WZ and ZX populations was less compared with BB population. Kinetic analyses showed that the WZ and ZX populations had higher substrate specificity compared with the BB population based on the values of k (cat) and k (cat) /K (m) to both reduced glutathione (GSH) and 1-chloro-2,4-dinitrobenzene (CDNB). The in vitro inhibition studies of GSTs indicated that the I (50) values of pyridaben from WZ and ZX populations of P. citri expressed 1.6- and 4.4-fold decreases, respectively, compared to the I (50) value of pyridaben from the BB population. In conclusion, all evidence suggested that the purified GSTs may partially contribute to the susceptibility of acaricide pyridaben in field populations of P. citri.

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Sublethal and transgenerational effects of pyridaben exposure on the fitness and gene expression of Panonychus citri

Deng

Xia

Luo

et al. 2023

Pest Management Science

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BACKGROUND Panonychus citri is a globally dominant citrus plant pest mite. Pesticide‐induced population resurgence is a concern for mite control. Exposure to sublethal concentrations of pesticides has stimulated reproduction and outbreak risks in many pests. Pyridaben, a mitochondrial electron transport inhibitor, has been frequently used worldwide in mite control. In the study, sublethal and transgenerational effects of pyridaben exposure on Pyr_Rs (resistant) and Pyr_Control (susceptible) strains were systematically investigated in both exposed parental generation (F0) and unexposed offspring generations (F1 and F2) by evaluating life‐table and physiological parameters. RESULTS After exposure to pyridaben, the fecundity of both strains was significantly reduced in F0 generation while significantly induced in F1 generation. Interestingly, these effects also stimulated the fecundity of the F2 generation in Pyr_Control strain while no significant effects occurred for Pyr_Rs strain. The intrinsic rate of increase (r) and finite rate of increase (λ) were significantly decreased only in F1 generation of Pyr_Control strain after exposure treatment. Meanwhile, the population projection indicated a smaller population size in F1 generation of Pyr_Control strain while a population increase for Pyr_Rs strain after sublethal treatment. Subsequent detoxification enzyme assays indicated that only P450 activities in F0 generation were significantly activated by LC30 exposure to pyridaben in both strains. Significant downregulation of reproduction‐related (Pc_Vg) genes was observed in the F0 generations of both strains. Significant upregulation of P450 (CYP4CL2) and Pc_Vg of the F1 generation in both strains suggested the presence of delayed hormesis effects on the reproduction and developed tolerance to pyridaben, although the effects did not last over a longer period (F2 generation). CONCLUSION These results provide evidence for transgenerational hormesis effects of low concentrations of pyridaben exposure that may lead to population increase and resurgence risks of resistant‐mites in natural settings by stimulating reproduction. © 2023 Society of Chemical Industry.

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Susceptibility and Activity of GlutathioneS-Transferases in Nine Field Populations ofPanonychus citri(Acari: Tetranychidae) to Pyridaben and Azocyclotin

Abstract: BioOne Complete (complete.BioOne.org) is a full-text database of 200 subscribed and open-access titles in the biological, ecological, and environmental sciences published by nonprofit societies, associations, museums, institutions, and presses.

Cited by 32 publications

References 31 publications

Purification and biochemical characterization of glutathione S‐transferases from four field populations of Bactrocera dorsalis (Hendel) (Diptera: Tephritidae)

Purification and biochemical characterization of glutathione S‐transferases from four field populations of Bactrocera dorsalis (Hendel) (Diptera: Tephritidae)

Purification and partial characterization of glutathione S-transferases from three field populations of Panonychus citri (Acari: Tetranychidae)

Sublethal and transgenerational effects of pyridaben exposure on the fitness and gene expression of Panonychus citri

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