Susceptibility in cell culture of feline immunodeficiency virus to eighteen antiviral agents

Smyth, Neil; McCracken, C.; Gaskell, R. M.; Js, Cameron; Coates, Jonathan; Gaskell, C. J.; Hart, C. A.; Bennett, Malcolm J.

doi:10.1093/jac/34.4.589

Cited by 18 publications

(21 citation statements)

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“…However, it did not inhibit FIV in vitro. Failure of a protease inhibitor (synthesized by Roche Laboratories) to inhibit FIV has been previously reported (Smyth et al, 1994). Cross-species inhibition of proteases by DG-35-VIII is likely to be influenced by the amino acid sequences of the enzymes themselves.…”

Section: Discussionmentioning

confidence: 95%

Antiviral Activity of DG-35-VIII, a Potent Inhibitor of the Protease of Human Immunodeficiency Virus

Grobelny

Chen

Tyssen

et al. 1997

Antivir Chem Chemother

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The protease of human immunodeficiency virus (HIV) is an important target for antiretroviral drug therapy. The synthesis and in vitro antiviral activity of a novel protease inhibitor, DG-35-VIII, which contains an hydroxyethylhydrazide core unit, is described. DG-35-VIII had potent activity against HIV-1 and related viruses (HIV-2 and simian immunodeficiency virus) in an acutely infected T lymphocyte line (MT-2) and was also active in cells chronically infected with HIV-1, where it inhibited processing of the Pr55gag and Pr160 gag-pal precursor proteins.

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Section: Discussionmentioning

confidence: 95%

Antiviral Activity of DG-35-VIII, a Potent Inhibitor of the Protease of Human Immunodeficiency Virus

Grobelny

Chen

Tyssen

et al. 1997

Antivir Chem Chemother

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“…Although FIV's overall genomic organization is more closely related to that of nonprimate lentiviruses, the biology of FIV resembles that of HIV, as it is highly lymphotropic and causes an immunodeficiency syndrome. Molecular cloning and characterization of the gene and gene products encoded by FIV make it amenable to molecular manipulations for detailed studies of the lentivirus life cycle and development of intervention strategies (30,39).…”

mentioning

confidence: 99%

Inhibitory Activity of Synthetic Peptide Antibiotics on Feline Immunodeficiency Virus Infectivity In Vitro

Kennedy‐Stoskopf

Jaynes³

et al. 2002

J Virol

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for up to 7 days, The virions from the D4E1-treated culture had impaired infectivity, as measured by the 50% tissue culture infectious dose and nested PCR analysis of proviral DNA. However, these noninfectious virions were able to bind and internalize, suggesting a defect at some postentry step. After chronically infected CrFK cells were treated with D4E1 for 24 h, increased cell-associated mature p26 Gag and decreased extracellular virus-associated p26 Gag were observed by Western blot analysis, suggesting that virus assembly and/or release may be blocked by D4E1 treatment, whereas virus binding, penetration, RNA synthesis, and protein synthesis appear to be unaffected. Synthetic peptide antibiotics may be useful tools in the search for antiviral drugs having a wide therapeutic window for host cells.

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“…This small-animal model is a useful and costeffective system for the study of lentivirus immunopathogenesis and antiviral interventions (4). FIV is susceptible to nucleoside analogues, such as ZDV, zalcitabine, didanosine, and lamivudine (3TC; ␤-D-2Ј,3Ј-dideoxy-3Ј-thiacytidine), in vitro at concentrations similar to those observed with HIV-1 (22,23,29,31). The sensitivities of FIV and HIV-1 to the active triphosphate forms of ZDV and 3TC are also similar (21,27).…”

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confidence: 88%

Domestic Cat Model for Predicting Human Nucleoside Analogue Pharmacokinetics in Blood and Seminal Plasma

Jordan¹,

Pereira²,

Cohen³

et al. 2001

Antimicrob Agents Chemother

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To establish whether a feline model can predict nucleoside analogue behavior in human semen, zidovudine (ZDV) and lamivudine (3TC) pharmacokinetic parameters (PKs) were determined in the blood and seminal plasma of healthy cats. Our results show considerable similarity in ZDV and 3TC PKs between cats and humans. As in humans, ZDV and 3TC tend to accumulate in feline seminal plasma. Area under the blood plasma concentration-time curve was predictive of seminal plasma excretion. The felid model offers a unique in vivo experimental alternative for investigating the pharmacokinetics of nucleoside analogues in the male genital tract.

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Susceptibility in cell culture of feline immunodeficiency virus to eighteen antiviral agents

Cited by 18 publications

References 0 publications

Antiviral Activity of DG-35-VIII, a Potent Inhibitor of the Protease of Human Immunodeficiency Virus

Antiviral Activity of DG-35-VIII, a Potent Inhibitor of the Protease of Human Immunodeficiency Virus

Inhibitory Activity of Synthetic Peptide Antibiotics on Feline Immunodeficiency Virus Infectivity In Vitro

Domestic Cat Model for Predicting Human Nucleoside Analogue Pharmacokinetics in Blood and Seminal Plasma

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