Susceptibility of Aedes aegypti (Diptera: Culicidae) to Acanthamoeba polyphaga (Sarcomastigophora: Acanthamoebidae)

Rott, Marilise Brittes; Caumo, Karin Silva; Sauter, Ismael Pretto; Eckert, Janina; Rosa, Luana da; Silva, Onilda da

doi:10.1007/s00436-010-1843-9

Cited by 4 publications

(2 citation statements)

References 22 publications

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“…Apart from intra-amoebic microorganisms, a wet-bench study investigated the ecological relation between amoeba and mosquitoes, demonstrating for the first time that A. polyphaga can infect Aedes aegypti . 94 Moreover, the role of FLA as vehicles of pathogenic microorganisms debated worldwide 95 , 96 , 97 and also present in Brazilian surveys 85 , 88 , 91 , 98 reinforce the need of monitoring FLA presence in the environment.…”

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confidence: 98%

A history of over 40 years of potentially pathogenic free-living amoeba studies in Brazil - a systematic review

Bellini

Thiemann

Reyes-Batllé

et al. 2022

Mem. Inst. Oswaldo Cruz

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Free-living amoeba (FLA) group includes the potentially pathogenic genera Acanthamoeba, Naegleria, Balamuthia, Sappinia, and Vermamoeba, causative agents of human infections (encephalitis, keratitis, and disseminated diseases). In Brazil, the first report on pathogenic FLA was published in the 70s and showed meningoencephalitis caused by Naegleria spp. FLA studies are emerging, but no literature review is available to investigate this trend in Brazil critically. Thus, the present work aims to integrate and discuss these data. Scopus, PubMed, and Web of Science were searched, retrieving studies from 1974 to 2020. The screening process resulted in 178 papers, which were clustered into core and auxiliary classes and sorted into five categories: wet-bench studies, dry-bench studies, clinical reports, environmental identifications, and literature reviews. The papers dating from the last ten years account for 75% (134/178) of the total publications, indicating the FLA topic has gained Brazilian interest. Moreover, 81% (144/178) address Acanthamoeba-related matter, revealing this genus as the most prevalent in all categories. Brazil's Southeast, South, and Midwest geographic regions accounted for 96% (171/178) of the publications studied in the present work. To the best of our knowledge, this review is the pioneer in summarising the FLA research history in Brazil.

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confidence: 98%

A history of over 40 years of potentially pathogenic free-living amoeba studies in Brazil - a systematic review

Bellini

Thiemann

Reyes-Batllé

et al. 2022

Mem. Inst. Oswaldo Cruz

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“…In figure 1.8, those reports grouped as in vitro comprise: gene silencing in Acanthamoeba (198); molecular detection of Acanthamoeba by showing randomly amplified polymorphic DNA (RAPD) as a suitable strategy to rapid genotyping new isolates; (196) metabolism studies in Naegleria (199)(200) and Acanthamoeba, (201)(202); interactions among FLA and intracellularly resistant microorganisms, (173,(203)(204)(205)(206); serach for antibodies as detection tool for Acanthamoeba infections, (207) and assays demonstrating amoeba capability of infecting mosquitoes, by using Acanthamoeba polyphaga and Aedes aegypti as studies of case. (208) In vitro assays by using natural substances as bacteriocin-like compounds (209) or synthetical substances as biguanides (e.g. : chlorhexidine) (210) in Acanthamoeba strains, whose doseresponse assays confirmed amoebostatic and cysticidal effects related to these substances, respectvilly to bacteriocin and biguanides.…”

Section: Epidemiologymentioning

confidence: 99%

Diversity of free-living amoebas in the Monjolinho River in São Paulo state – morphological and molecular approaches

Bellini¹

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I kindly thank my Brazilian advisor Prof. Otavio Henrique Thiemann, his knowledge and all advisements that contributed to the development of the present PhD study. I thanks for the many years of guidance that enriched my concept of making science and I thank the opportunity he gave to me of conducting the research in my ways. I deeply thank my Spanish advisor Prof. Jacob Lorenzo-Morales, whose support, suggestions, and expertise to guide my scientific steps were invaluable. I thanks for the enthusiasm to discuss science, for his availability to receive me at his laboratory, and all opportunities he provided to me enabling the enrichment of my PhD research. I sincerely thank both supervisors for having settled the cotutelle agreement, the origin of the present Double Degree PhD. I thank you both for all assistance, professional instructions, and encouragement along my PhD journey.The laboratory members: I thank all members of the Structural Biology Laboratory (USP). You all have helped in different manners, sharing the daily lab journey and motivating to go further. I thank you for each scientific discussion, exchange of knowledge, and opportunity to enhance scientific abilities. I thanks to Ivan, Marco Túlio, Tatiana, Evandro, Diego, Murilo, Vitor, Witer, and Fernanda. Special thanks go to Ana Letícia and Matheus Issa, undergraduate students who have been working with me. It has been an exciting challenge and a great opportunity to develop manners to teach scientific skills. I have also learned a lot with you both, thank you! I thank Dra. Susana Sculaccio, for her extensive support and technical expertise, shared with me during my scientific development. I also thank her for years of friendship. As well for Dr. Humberto, I thank his support in the laboratory routine. Also, a special thank to the Biophysics Laboratory at IFSC/USP assistance, in persons of Bel, Andressa, Rafael! My gratitude to all members of the Free Living Amoeba Laboratory

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Prevalence of Acanthamoeba spp. (Sarcomastigophora: Acanthamoebidae) in wild populations of Aedes aegypti (Diptera: Culicidae)

et al. 2012

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Studies of interrelationship between microorganisms and mosquitoes are of great importance, since it can provide support for better understand related to biology, development and their control. In this way, it is known that mosquito larvae and free-living amoebae (FLA) normally occupy similar aquatic microhabitats. However, few studies have been conducted about such coexistence. For that reason, the objective of the present study was to verify the prevalence of Acanthamoeba spp. in wild populations of Aedes aegypti, as well as to characterize the genotypic lineage, and their possible pathogenicity through thermo- and osmotolerance. Amoebae were investigated in 60 pools, each containing ten larvae of A. aegypti, collected in Porto Alegre (Rio Grande do Sul, Brazil). The Acanthamoeba isolates were morphologically characterized and submitted to the polymerase chain reaction technique to confirm identification of the genus. In addition, genotype analyses as well as tests for presumptive pathogenicity in some samples were performed. Of the 60 pools examined, 54 (90 %) were positive for FLA. Of these isolates, 47 (87 %) belonged to the genus Acanthamoeba. The genotypic groups T4, T3 and T5 were identified, numbering 14 (53.8 %), ten (38.5 %) and two (7.7 %) isolates, respectively. The physiological tests performed with 14 strains showed that 12 (85.7 %) were non-pathogenic, while two (14.3 %) were considered as having low pathogenic potential. These results provide a basis for a better understanding of the interaction between these protozoan and mosquitoes in their natural habitat. This study is the first to report the isolation of Acanthamoeba spp. from wild mosquitoes.

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Susceptibility of Aedes aegypti (Diptera: Culicidae) to Acanthamoeba polyphaga (Sarcomastigophora: Acanthamoebidae)

Cited by 4 publications

References 22 publications

A history of over 40 years of potentially pathogenic free-living amoeba studies in Brazil - a systematic review

A history of over 40 years of potentially pathogenic free-living amoeba studies in Brazil - a systematic review

Diversity of free-living amoebas in the Monjolinho River in São Paulo state – morphological and molecular approaches

Prevalence of Acanthamoeba spp. (Sarcomastigophora: Acanthamoebidae) in wild populations of Aedes aegypti (Diptera: Culicidae)

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Susceptibility of Aedes aegypti (Diptera: Culicidae) to Acanthamoeba polyphaga (Sarcomastigophora: Acanthamoebidae)

Cited by 4 publications

References 22 publications

A history of over 40 years of potentially pathogenic free-living amoeba studies in Brazil - a systematic review

A history of over 40 years of potentially pathogenic free-living amoeba studies in Brazil - a systematic review

Diversity of free-living amoebas in the Monjolinho River in São Paulo state &ndash; morphological and molecular approaches

Prevalence of Acanthamoeba spp. (Sarcomastigophora: Acanthamoebidae) in wild populations of Aedes aegypti (Diptera: Culicidae)

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Diversity of free-living amoebas in the Monjolinho River in São Paulo state – morphological and molecular approaches