Susceptibility of Aedes albopictus and Culex quinquefasciatus to Japanese encephalitis virus

Hernández‐Triana, Luis M.; Folly, Arran J.; Sewgobind, Sanam; Lean, Fabian Z. X.; Ackroyd, Stuart; Núñez, Alejandro; Delacour, Sarah; Drago, Andrea; Visentin, Patrizia; Mansfield, Karen L.; Johnson, Nicholas

doi:10.1186/s13071-022-05329-0

Cited by 13 publications

(8 citation statements)

References 36 publications

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“…On the other hand, it is interesting to note that models by Samy et al (2016) and Alaniz et al (2018) have identified temperate regions, including Ireland, as potentially suitable for Cx. quinquefasciatus ; findings that are supported by its detection in Turkey, which has European territory (Gunay et al 2015; Hernández-Triana et al 2022). In the USA, where Cx.…”

Section: Discussionmentioning

confidence: 81%

DNA barcoding reveals an increased diversity within the genusCulex(Diptera: Culicidae) in Ireland

Curran,

O’Hanlon,

Gallagher

et al. 2023

Preprint

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Arthropod populations are changing globally due to the combined impacts of climate change, land-use transformation, globalisation and trade. Changes in arthropod populations can potentially result in the decline and extinction of some native and/or endemic species, while other species that potentially pose threats may increase. Such dynamics lead to weakened ecosystem resilience, which can facilitate the establishment and spread of species with vector capabilities, such as mosquitoes. While many of the mosquito species present in Ireland possess vector capabilities, populations have not been nationally surveyed since 1991. At that time, species identification relied on morphological examination, a method that was even then recognised as inadequate for differentiating certain species within complexes. In this study, we present the first DNA barcoding investigation of Irish mosquito populations and reveal species and hidden diversity not previously recognised in Ireland. These include two potential new species/ecotypes,Culex torrentiumandCulex pipiensformpipiens, which were recorded for the first time in Ireland. We demonstrate that barcoding with Cytochrome C Oxidase Subunit 1 (COI) based primers is suitable for the majority of species-level identifications. However, additional primer sets targeting the Internal Transcribed Spacer regions 1 and 2 (ITS1 and ITS2) were necessary to differentiate species within theCulex pipienscomplex. The multi-locus approach employed in this study can enhance national surveillance efforts, especially in monitoring mosquitoes that may transmit vector-borne diseases, and in recognising increased species diversity from a biodiversity perspective.

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Section: Discussionmentioning

confidence: 81%

DNA barcoding reveals an increased diversity within the genusCulex(Diptera: Culicidae) in Ireland

Curran,

O’Hanlon,

Gallagher

et al. 2023

Preprint

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“…Mosquito infections were performed in the BSL-3 laboratory of the Zoonosis Science Centre in accordance with previous studies [ 23 , 46 – 48 ]. Female mosquitoes aged 7–14 days were deprived of sugar solution for 24 h before being presented with an infectious blood meal.…”

Section: Methodsmentioning

confidence: 99%

Vector competence of Swedish Culex pipiens mosquitoes for Japanese encephalitis virus

Krambrich,

Akaberi,

Lindahl

et al. 2024

Parasites Vectors

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Background Japanese encephalitis virus (JEV) is an emerging mosquito-borne Orthoflavivirus that poses a significant public health risk in many temperate and tropical regions in Asia. Since the climate in some endemic countries is similar to temperate climates observed in Europe, understanding the role of specific mosquito species in the transmission of JEV is essential for predicting and effectively controlling the potential for the introduction and establishment of JEV in Europe. Methods This study aimed to investigate the vector competence of colonized Culex pipiens biotype molestus mosquitoes for JEV. The mosquitoes were initially collected from the field in southern Sweden. The mosquitoes were offered a blood meal containing the Nakayama strain of JEV (genotype III), and infection rates, dissemination rates, and transmission rates were evaluated at 14, 21, and 28 days post-feeding. Results The study revealed that colonized Swedish Cx. pipiens are susceptible to JEV infection, with a stable infection rate of around 10% at all timepoints. However, the virus was only detected in the legs of one mosquito at 21 days post-feeding, and no mosquito saliva contained JEV. Conclusions Overall, this research shows that Swedish Cx. pipiens can become infected with JEV, and emphasizes the importance of further understanding of the thresholds and barriers for JEV dissemination in mosquitoes. Graphical Abstract

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“…( Och .) dorsalis and Culiseta ( Culiseta ) annulata ) have shown potential as vectors in laboratory settings (Chapman et al., 2020; de Wispelaere et al., 2017; Hernandez‐Triana et al., 2022; Huber et al., 2014; Mackenzie‐Impoinvil et al., 2015). While Ae .…”

Section: Likelihood Of Transmission Of the Virus In The Eumentioning

confidence: 99%

Potential for emergence of Japanese encephalitis in the European Union

Gossner,

Dhollander,

Presser

et al. 2023

Zoonoses and Public Health

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Background and objectiveNo autochthonous human cases of Japanese encephalitis (JE) have been reported to date in the European Union (EU). In this study, we assess the likelihood of Japanese encephalitis virus (JEV) introduction and transmission within the EU and propose outbreak response measures.Risk assessmentGiven the global geographical distribution of JEV, the probability of virus introduction into the EU is currently very low, with viremic bird migration being the most plausible pathway of introduction. However, this likelihood would significantly increase if the virus were to become established in the Middle East, Caucasus, Central Asia or Africa. Considering the environmental conditions that are expected to be conducive for virus circulation, there is a high likelihood of virus transmission within the EU after its introduction in environmentally suitable areas. The spread of the virus within the EU would likely occur through the movement of wild birds, pigs and mosquitoes.MitigationTo mitigate or potentially contain the emergence of JE in the EU, early detection of both human and animal cases will be crucial.

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Susceptibility of Aedes albopictus and Culex quinquefasciatus to Japanese encephalitis virus

Cited by 13 publications

References 36 publications

DNA barcoding reveals an increased diversity within the genusCulex(Diptera: Culicidae) in Ireland

DNA barcoding reveals an increased diversity within the genusCulex(Diptera: Culicidae) in Ireland

Vector competence of Swedish Culex pipiens mosquitoes for Japanese encephalitis virus

Potential for emergence of Japanese encephalitis in the European Union

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