Susceptibility of Chicken Embryos to Highly Virulent Infectious Bursal Disease Virus.

Clinical and Laboratory Diagnoses of Newcastle and Infectious Bursal Diseases of Chickens

“…In positive cases the embryos were died within 24 to 96 hours of post-inoculation. The CAM was thickened, dead embryos were congested and hemorrhagic (petechial and ecchymotic) along the feather tracts, toe and cerebral area which were similar to the findings of Hitchner (1970) and Takase et al (1996). The reduced rate of virus isolation may be due to absence or low concentration of virus in the remaining six inoculums or due to the presence of maternal antibody in the embryonated eggs (Rosales et al, 1989).…”

Characterization of the antigenic, immunogenic, and pathogenic variation of infectious bursal disease virus due to propagation in different host systems (bursa, embryo, and cell culture). III. Pathogenicity

“…Results obtained in the present study were similar to those shown by other researchers who reported a decrease in the pathogenicity of IBDV strains propagated in embryos and cell cultures (Müller et al, 1986;Becht & Müller, 1991;Ismail & Saif, 1991;Tsai & Saif, 1992;Tanimura et al, 1995;Bayyari et al, 1996;Hassan & Saif, 1996;Hassan et al, 1996a,b;Takase et al, 1996;Yamaguchi et al, 1996;Chen et al, 1998). However, antigenic and/or immunogenic changes were not recognized in these studies.…”

“…Differences in the relative pathogenicity of IBDV that are apparently related to the host system utilized have been previously reported (Müller et al, 1986;Becht & Müller, 1991;Ismail & Saif, 1991;Tsai & Saif, 1992;Tanimura et al, 1995;Bayyari et al, 1996;Hassan et al, 1996a,b;Hassan & Saif, 1996;Takase et al, 1996;Yamaguchi et al, 1996;Chen et al, 1998;Fussell, 1998). However, these authors did not correlate changes in relative pathogenicity with either antigenic or immunogenic differences.…”

Characterization of variant infectious bursal disease virus from a broiler farm in Japan using immunized sentinel chickens

“…The VN cross-reactivity test was performed by α neutralization procedure (constant-serum diluted-virus method) using 11-day-old SPF embryonated eggs with the isolated IBDV TY2 strain, IBDV K strain, very virulent IBDV F539 strain [ 23 , 24 ] and antisera prepared from convalescent SPF chickens infected with either the IBDV TY2 or IBDV K strain. Serial ten-fold dilutions (10 −1 –10 −6 ) of each IBDV were prepared, and each dilution was mixed with the same volume of either antiserum (diluted 5 times) and maintained at 37°C for one hr.…”