Susceptibility of clinical isolates of Candida to photodynamic effects of curcumin

Abstract

Background and ObjectiveThe resistance of Candida species to antifungals represents a major challenge for therapeutic and prophylactic strategies. This study evaluated photodynamic therapy (PDT) mediated by Curcumin (CUR) against clinical isolates of C. albicans, C. tropicalis, and C. glabrata, both in planktonic and biofilm forms.Study Design/Materials and MethodsSuspensions of Candida were treated with three CUR concentrations and exposed to four LED fluences. The protocol that showed the best outcomes for inactivation of the planktonic phase was selected to be evaluated against Candida biofilms. In addition, two higher CUR concentrations were tested. The metabolic activity of biofilms was evaluated by means of XTT reduction assay and the biofilm biomass was evaluated using crystal violet (CV) staining assay. Data were analyzed in a mixed model nested ANOVA, Wilcoxon's nonparametric tests, and the Kruskal–Wallis test (α = 5%).ResultsThe use of CUR in association with light was able to promote a significant antifungal effect against the planktonic form of the yeasts. When using 40 µM of CUR, the metabolic activity of C. albicans, C. glabrata, and C. tropicalis biofilms was reduced by 85%, 85%, and 73%, respectively, at 18 J/cm2. CUR‐mediated PDT also decreased the biofilm biomass of all species evaluated. In addition, CV staining showed that C. albicans isolates were strong biofilm‐forming strains, when compared with C. glabrata and C. tropicalis isolates.ConclusionThe results from the present investigation showed that low CUR concentrations can be highly effective for inactivating Candida isolates when associated with light excitation. Lasers Surg. Med. 43:927‐934, 2011. © 2011 Wiley Periodicals, Inc.

Photoinactivation of Candida albicans using methylene blue as photosensitizer

“…The light therapeutic properties studies with antimicrobial purpose are primarily motivated by resistance cases of conventional antimicrobial 3,6,[11][12][13][14] . The need to overcome these deficiencies led to the exploration of Petri dishes containing Agar Sabouraud Dextrose culture medium.…”

“…Several studies that evaluated azole-resistant Candida species, noted that these strains were sensitive to PDT [11][12][19][20] . The photosensitizer and light application specifically on the site affected by candidiasis, dramatically reduces the local and systemic side effects, allowing the photodynamic therapy to be seen as a promising treatment against Candida albicans 21 .…”

Validation of Photodynamic Action via Photobleaching of a New Curcumin-Based Composite with Enhanced Water Solubility

“…Recently, Dovigo et.al. demonstrated the antibacterial photodynamic effect against Candida Albicans [16][17][18], reinforcing the curcumin photodynamic properties. The effect of curcumin on gram-positive (GP) and gram-negative (GN) bacteria was also investigated, in which Enterococcus faecalis (GP) and Escherichia coli (NG) were used as targets.…”

Efeitos da Terapia Fotodinâmica Antimicrobiana sobre Microrganismos Cariogênicos: Estudo Preliminar

“…Baseado no fato de que cárie dentaria é capaz de destruir estruturas mineralizadas em qualquer estágio da vida, crianças são mais afetadas por essa doença devido a imaturidade de sua destreza manual e, como consequência, não atingindo um nível ótimo de remoção de placa dentária ou biofilme [15]. Embora um estudo recente tenha provado a resistência de isolados clínicos à TFA [13], nenhum estudo prévio focou-se no efeito da TFA sobre isolados clínicos de microrganismos cariogênicos oriundo da cavidade bucal de crianças.…”

“…Baseado no fato de que cárie dentaria é capaz de destruir estruturas mineralizadas em qualquer estágio da vida, crianças são mais afetadas por essa doença devido a imaturidade de sua destreza manual e, como consequência, não atingindo um nível ótimo de remoção de placa dentária ou biofilme [15]. Embora um estudo recente tenha provado a resistência de isolados clínicos à TFA [13], nenhum estudo prévio focou-se no efeito da TFA sobre isolados clínicos de microrganismos cariogênicos oriundo da cavidade bucal de crianças.Portanto, o presente estudo teve como objetivo investigar o efeito da TFA mediado por azul de toluidina (TBO) em associação a diodos emissores de luz (LEDs) no comprimento de onda vermelho sobre uma cepa padrão de S. mutans e comparar a dois isolados clínicos de estreptococos da saliva de crianças. …”