Susceptibility of different TMEM154 genotypes in three Italian sheep breeds infected by different SRLV genotypes

Moretti, Rıccardo; Sartore, Stefano; Colitti, Barbara; Profiti, Margherita; Chessa, S.; Rosati, Sergio; Sacchi, Paola

doi:10.1186/s13567-022-01079-0

Cited by 7 publications

(9 citation statements)

References 29 publications

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“…The prospective cohort study results present here were entirely consistent with previous reports of retrospective matched cases control studies [ 4 , 13 ] and related prospective cohort studies [ 6 , 7 , 8 , 9 , 10 , 11 , 12 , 20 ]. Ewes with two copies of haplotype “1” show significantly reduced susceptibility to lentivirus infection compared to those with one or two copies of haplotypes “2” or “3” reported.…”

Section: Discussionsupporting

confidence: 92%

“…The deletion haplotype “4” typically has a frequency less than 5% in sheep populations and has not been rigorously tested for association with infection. However, these association with haplotypes “1”, “2”, and “3” have been reproduced around the world with different sheep populations, viruses, and management environments [ 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 ]. While other sheep variants have been reported to be associated with OPP, replication and validation in prospective challenge studies is lacking [ 14 , 15 , 16 , 17 , 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

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Impact of Four Ovine TMEM154 Haplotypes on Ewes during Multiyear Lentivirus Exposure

Freking

Murphy²,

Chitko-McKown³

et al. 2022

IJMS

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Polypeptide variation encoded by the ovine transmembrane protein 154 gene (TMEM154) is associated with susceptibility to ovine lentivirus, the causative agent of Ovine Progressive Pneumonia (OPP) and Visna/Maedi. Our aim was to compare the four most prevalent TMEM154 haplotypes on the incidence of infection and ewe productivity during natural multiyear virus exposure. Prospective cohort studies were designed to test gene action and estimate effects of TMEM154 haplotypes encoding distinctive variant residues: K35 (“1”), I70 (“2”), ancestral (“3”), and A4del/M44 (“4”). Exposure consisted of co-mingling infected ewes at a rate greater than 30% with serological status evaluated every four months. For ewes with one or two copies of the highly susceptible haplotypes “2” and ”3”, the infection prevalence steadily increased to nearly 100% at 55 months. Haplotypes “2” and “3” were equally susceptible and dominant to haplotype “1”. A difference was not detected (p < 0.53) in the magnitude of effect with haplotype combinations of “1” and ”4”. The ewe infection prevalence with “1,1”; “1,4”; and “4,4” was 10% to 40% at 55 months. The latter suggested that two copies of the K35 amino acid substitution (“1”) were as effective as a homozygous TMEM154 “knockout” with the frame-shift deletion mutation (“4”) in reducing infection susceptibility. When considering ewe reproductive performance, a difference was not detected when comparing haplotypes “2”, and “3” to each other, or “1” and “4” to each other. Our study indicated that ewes with two copies of the severely truncated versions of TMEM154 (“4,4”) had normal lamb productivity. Without complete understanding of the natural function of TMEM154 our recommendations to producers interested in using TMEM154 selection to reduce their flock’s genetic predisposition to OPP are encouraged to increase the frequency of TMEM154 haplotype K35 (“1”) since it encodes a full-length protein with minimal difference to the ancestral polypeptide.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Impact of Four Ovine TMEM154 Haplotypes on Ewes during Multiyear Lentivirus Exposure

Freking

Murphy²,

Chitko-McKown³

et al. 2022

IJMS

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“…There is evidence that the host’s genetic background plays an important role in determining susceptibility or resistance to SRLV infection and pathogenesis. Some breeds (Texel, Border Leicester, Finnish Landrace, Biellese, Churra and Assaf) are more susceptible to SRLV infection than others (Rabouillet, Île de France, Suffolk Columbia, Rambo, Polipay, Delle Langhe, Bergamasca, Raza Navarra and Aragonesa) ( 69 , 83 , 106 , 147 ). Furthermore, many authors reported the presence of loci linked to SRLV susceptibility or resistance, such as ZNF389 ( 174 ), DRB1 ( 85 ), TLR9 ( 160 ), DPPA2/DPPA4 and SYTL3 ( 173 ), TMEM154 ( 69 ) and CCR5 ( 175 ).…”

Section: Tropism and Pathologymentioning

confidence: 99%

“…( 103 ) and Moretti et al . ( 106 ) suggested that the relationship between the single nucleotide polymorphism (SNP) TMEM154_E35K and susceptibility or resistance must be treated with caution, since in the German Merinoland breed and the Italian Biellese, Delle Langhe and Bergamasca breeds, a large number of sheep with the less susceptible genotype were seropositive. Ramirez et al .…”

Section: Tropism and Pathologymentioning

confidence: 99%

The genetic variability of small-ruminant lentiviruses and its impact on tropism, the development of diagnostic tests and vaccines and the effectiveness of control programmes

Olech

2023

Journal of Veterinary Research

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Introduction Maedi-visna virus and caprine arthritis encephalitis virus are two closely related lentiviruses which cause multisystemic, progressive and persistent infection in goats and sheep. Because these viruses frequently cross the species barrier, they are considered to be one genetic group called small-ruminant lentiviruses (SRLV). They have in vivo tropism mainly for monocytes and macrophages and organ tropism with unknown mechanisms. Typical clinical signs are pneumonia in sheep, arthritis in goats, and mastitis in both species. Infection with SRLV cannot currently be treated or prevented, and control programmes are the only approaches to avoiding its spread. These programmes rely mainly on annual serological testing and elimination of positive animals. However, the high genetic and antigenic variability of SRLV complicate their early and definitive diagnosis. The objective of this review is to summarise the current knowledge of SRLV genetic variation and its implications for tropism, the development of diagnostic tests and vaccines and the effectiveness of control and eradication programmes. Material and Methods Subject literature was selected from the PubMed and the Google Scholar databases. Results The high genetic diversity of SRLV affects the performance of diagnostic tools and therefore control programmes. For the early and definitive diagnosis of SRLV infection, a combination of serological and molecular tests is suggested. Testing by PCR can also be considered for sub-yearling animals. There are still significant gaps in our knowledge of the epidemiology, immunology and biology of SRLV and their impact on animal production and welfare. Conclusion This information may aid selection of the most effective SRLV spread reduction measures.

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“…Isso pode estar relacionado com a existência de outras mutações no gene TMEM154 diferentes da E35K tais como D33N, T44M, I70N ou G38R, porém os envolvimentos desses SNPs individualmente ou juntamente são ainda considerados desconhecidos para susceptibilidade a LVPR(RAMÍREZ et al, 2021). Foi observado que nenhum vírus do genótipo A foi encontrado em ovinos com o genótipo KK, independentemente da raça, que é consistente com o fator de proteção, enquanto que poderia haver uma potencial susceptibilidade em animais carregando pelo menos um alelo (EE ou EK) em infecções causadas pelo genótipo B e E(MORETTI et al, 2022). podem também contribuir para a replicação viral.…”

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>b<Estudo dos diferentes genótipos de lentivírus de pequenos ruminantes no Brasil e sua relação com manifestações clínicas>/b<

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Ao meu Senhor, Pai dos Céus, agradeço e louvo sempre pela graça derramada na minha vida, e por chegar até aqui, pois tudo posso naquele que me fortalece! Á Universidade de São Paulo, gratidão pela acolhida e oportunidade por também fazer parte da sua história.Á minha orientadora, Profa. Dra. Lilian Gregory, pela oportunidade que me foi concedida para trabalhar com a senhora, pela confiança depositada e os inúmeros momentos de aprendizado pessoal e profissional.Aos meus amigos da equipe Profa.

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Susceptibility of different TMEM154 genotypes in three Italian sheep breeds infected by different SRLV genotypes

Cited by 7 publications

References 29 publications

Impact of Four Ovine TMEM154 Haplotypes on Ewes during Multiyear Lentivirus Exposure

Impact of Four Ovine TMEM154 Haplotypes on Ewes during Multiyear Lentivirus Exposure

The genetic variability of small-ruminant lentiviruses and its impact on tropism, the development of diagnostic tests and vaccines and the effectiveness of control programmes

>b<Estudo dos diferentes genótipos de lentivírus de pequenos ruminantes no Brasil e sua relação com manifestações clínicas>/b<

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