Susceptibility of Escherichia coli and Clostridium perfringens to sucrose monoesters of capric and lauric acid

Skřivanová, E.; Pražáková, Š.; Benada, Oldřich; Hovorková, P.; Marounek, M.

doi:10.17221/7588-cjas

Cited by 10 publications

(9 citation statements)

References 30 publications

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“…The fatty acid glyceride product applied in this experiment is a mixture of monoglycerides (C4, C8, and C10) and has total glycerides of butyric and medium-chain fatty acid content of ~ 45%). Monobutyrin and some of the fatty acids in this product can be effective on the reduction of E. coli and C. perfringens ( Namkung et al., 2011 ; Skřivanová et al., 2014 ). In the present study, the addition of both levels of fatty acid glyceride product improved the overall feed efficiency compared to the nonsupplemented group.…”

Section: Discussionmentioning

confidence: 99%

“…Membrane-destabilizing activity causes increased cell permeability and cell lysis, leading to cell death ( Yoon et al., 2018 ). Monoglycerol compounds have shown to reduce numbers of Salmonella typhimurium ( Tosi et al., 2016 ) Campylobacter jejuni ( Hilmarsson et al., 2006 ), E. coli ( Thormar et al., 2006 ) and C. perfringens ( Skřivanová et al., 2014 ) in chickens.…”

Section: Introductionmentioning

confidence: 99%

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Buffered formic acid and a monoglyceride blend coordinately alleviate subclinical necrotic enteritis impact in broiler chickens

Gharib-Naseri

Kheravii²,

Li³

et al. 2021

Poultry Science

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The objective of this study was to evaluate the effect of 2 different doses of a partially buffered formic acid product (Amasil NA; 61% formic acid, 20.5% sodium formate), and a monoglyceride blend of short- and medium-chain fatty acids (BalanGut LS P) on necrotic enteritis ( NE ) infected broilers in terms of performance, intestinal microbial population and short-chain fatty acids concentrations in the gastrointestinal tract. A total of 528-day-old as hatched Ross 308 broilers were allocated to 48 pens with 11 birds in each pen. Six dietary treatments applied in the study were: T1) nonsupplemented diet (Control); T2) antibiotic supplemented diets; T3) and T4) high (Starter: 0.5%; Grower and Finisher: 0.5%) and low (Starter: 0.3%; Grower and Finisher: 0.2%) dose of Amasil NA; and groups T5) and T6) high (Starter: 0.3%; Grower and Finisher: 0.2%) and low dose (Starter: 0.3%; Grower: 0.15%; Finisher: 0.075%) of (BalanGut LS P). All birds in this study were fed starter (d 0-10), grower (d 11–24) and finisher (d 25–35) diets and challenged with NE. To induce subclinical NE, oral administrations of Eimeria oocysts (d 9) followed by inoculation of Clostridium perfringens strains (d 14 and 15) were applied. Results showed that birds fed the high dose of Amasil NA, had a higher feed conversion ratio ( FCR, P < 0.05) compared to the nonsupplemented group during the starter period. Antibiotic supplementation reduced FCR during the grower ( P < 0.001), finisher ( P < 0.05) and overall ( P < 0.001) periods of the experiment. Both levels of BalanGut LS P and low levels of Amasil NA enhanced overall FCR ( P < 0.05) compared to the birds in the nonsupplemented group. Compared to the nonsupplemented group, high levels of Amasil NA and low levels of BalanGut LS P improved FCR in the finisher stage ( P < 0.05). On d 16, cecum digesta of birds fed with antibiotic supplemented diets showed a significantly lower number of C. perfringens ( P < 0.001) compared to the nonsupplemented and high level of BalanGut LS P group. Bacillus ( P < 0.01) and Ruminococcus numbers were significantly lower in the birds fed with high level of Amasil NA ( P < 0.05) compared to the antibiotic supplemented diets. High doses of Amasil NA, showed the highest propionate concentration in the cecum ( P < 0.001). The study suggests that supplementation of BalanGut LS P and Amasil NA at different feeding phases may achieve optimal performance improvement in broilers under NE challenge.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Buffered formic acid and a monoglyceride blend coordinately alleviate subclinical necrotic enteritis impact in broiler chickens

Gharib-Naseri

Kheravii²,

Li³

et al. 2021

Poultry Science

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“…The antimicrobial activity was also proved by Skrivanova et al 2014 for the sucrose monoesters of capric and lauric acids. In their experiments, a significantly decreased number of viable cells of E. coli was found at all tested concentrations of sucrose monocapate (0.1-5 mg/ml).…”

Section: Zootechnical Additivesmentioning

confidence: 97%

Current use of phytogenic feed additives in animal nutrition: a review

Karásková¹,

Suchý²,

Straková³

2015

Czech J. Anim. Sci.

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A rapid development in the potential use of phytogenic feed additives has occurred mainly due to the 2006 EU ban on the use of antimicrobial substances as growth stimulators; however, they are also used as sensory, technological additives or substances positively affecting the quality of animal products. The use of phytogenic additives in form of extracts, predominantly essential oils, has been tested in a number of animal species; however, there is still a lack of scientific findings on the safety and efficacy of their use, or on their effect on the quality of animal products. The aim of this review was to sum up current scientific knowledge on phytogenic additives in animal nutrition.

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“…Skrivanova et al (2005) estimated the effective concentration of lauric acid for the inhibition of this pathogen at 0.04 mg/ml, in another study (Skrivanova et al 2014) a 30-minute incubation with lauric acid (2 mg/ml) revealed disintegration of the cell wall in some cells of C. perfringens. However, cytoplasmic membrane appeared to remain intact.…”

Section: Discussionmentioning

confidence: 98%

Determination of in vitro antibacterial activity of plant oils containing medium-chain fatty acids against Gram-positive pathogenic and gut commensal bacteria

Hovorková¹,

Laloučková²,

Skřivanová³

2018

Czech J. Anim. Sci.

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Hovorková P., Laloučková K., Skřivanová E. (2018): Determination of in vitro antibacterial activity of plant oils containing medium-chain fatty acids against Gram-positive pathogenic and gut commensal bacteria. Czech J. Anim. Sci., 63, 119-125.Increasing antibiotic resistance has led to a ban on antibiotic use in feed additives in the EU. Therefore, new non-antibiotic, pathogen-inhibiting agents are urgently needed. Inhibitory effects of eight plant oils containing medium-chain fatty acids (MCFAs) were evaluated against Gram-positive pathogenic and beneficial bacteria. The oils tested were palm, red palm, palm kernel (Elaeis guineensis), coconut (Cocos nucifera), babassu (Attalea speciosa), murumuru (Astrocaryum murumuru), tucuma (Astrocaryum vulgare), and Cuphea oil (Cuphea ignea); the method used was broth microdilution, and the findings were expressed as minimum inhibitory concentration (80%). Both hydrolyzed and unhydrolyzed forms of the oils were tested. MCFA hydrolysis was catalyzed by porcine pancreas lipase. The selective effect of the hydrolyzed forms of tested oils was highly evident. While the hydrolyzed oils were active against all tested bacteria (Clostridium perfringens, Enterococcus cecorum, Listeria monocytogenes, and Staphylococcus aureus), at 0.14-4.5 mg/ml, the same oils did not show any effect on commensal bacteria (Bifidobacterium spp. and Lactobacillus spp.). Tucuma and Cuphea seed oils showed the strongest antibacterial activity. Unhydrolyzed forms of all tested oils exerted no antibacterial effect against any test bacteria. This study, thus, forms a basis for the development of selective inhibitors in animal husbandry.

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Susceptibility of Escherichia coli and Clostridium perfringens to sucrose monoesters of capric and lauric acid

Cited by 10 publications

References 30 publications

Buffered formic acid and a monoglyceride blend coordinately alleviate subclinical necrotic enteritis impact in broiler chickens

Buffered formic acid and a monoglyceride blend coordinately alleviate subclinical necrotic enteritis impact in broiler chickens

Current use of phytogenic feed additives in animal nutrition: a review

Determination of in vitro antibacterial activity of plant oils containing medium-chain fatty acids against Gram-positive pathogenic and gut commensal bacteria

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