Susceptibility of organ cultures from chicken tissues for strains of infectious bronchitis virus isolated from the intestine

Bhattacharjee, Partha; Jones, Richard C.

doi:10.1080/03079459708419234

Cited by 7 publications

(5 citation statements)

References 17 publications

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“…However, susceptibility of these organs to IBV can be influenced by the strain of the virus and the amount of virus presence in the sample (infective dose). While a study suggested the universality of using kidney, bursa, and proventriculus in growing IBV, a poor result was obtained when IBV was propagated in cultures derived from different intestinal segments [ 61 ]. An advantage of this method includes easy titration and serotyping of IBV, since no virus adaptation is required [ 62 ].…”

Section: Diagnosismentioning

confidence: 99%

Pathogenesis and Diagnostic Approaches of Avian Infectious Bronchitis

Bande

Arshad

Omar

et al. 2016

Advances in Virology

107

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Infectious bronchitis (IB) is one of the major economically important poultry diseases distributed worldwide. It is caused by infectious bronchitis virus (IBV) and affects both galliform and nongalliform birds. Its economic impact includes decreased egg production and poor egg quality in layers, stunted growth, poor carcass weight, and mortality in broiler chickens. Although primarily affecting the respiratory tract, IBV demonstrates a wide range of tissues tropism, including the renal and reproductive systems. Thus, disease outcome may be influenced by the organ or tissue involved as well as pathotypes or strain of the infecting virus. Knowledge on the epidemiology of the prevalent IBV strains in a particular region is therefore important to guide control and preventions. Meanwhile previous diagnostic methods such as serology and virus isolations are less sensitive and time consuming, respectively; current methods, such as reverse transcription polymerase chain reaction (RT-PCR), Restriction Fragment Length Polymorphism (RFLP), and sequencing, offer highly sensitive, rapid, and accurate diagnostic results, thus enabling the genotyping of new viral strains within the shortest possible time. This review discusses aspects on pathogenesis and diagnostic methods for IBV infection.

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Section: Diagnosismentioning

confidence: 99%

Pathogenesis and Diagnostic Approaches of Avian Infectious Bronchitis

Bande

Arshad

Omar

et al. 2016

Advances in Virology

107

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“…Several strains of IBV have been isolated from cloacal swabs, faeces and caecal tonsils Alexander et al, 1978;Cook, 1984;Lucio & Fabricant, 1990). In vitro explants of several gut tissues have been shown to support the growth of IBV (Bhattacharjee, 1994;Bhattacharjee & Jones, 1997;Darbyshire et al, 1976). In studies where virus isolation was attempted from several tissues, maximum virus isolations were obtained from the oesophagus of chickens infected with ECV2, an enteric isolate of IBV (Lucio & Fabricant, 1990) and 793/B-like virus (Dhinakar Raj & Jones, 1996a).…”

Section: Virus In the Intestinementioning

confidence: 99%

Infectious bronchitis virus: Immunopathogenesis of infection in the chicken

1997

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SUMMARYThe immunopathogenesis of infectious bronchitis virus (IBV) infection in the chicken is reviewed. While infectious bronchitis (IB) is considered primarily a disease of the respiratory system, different IBV strains may show variable tissue tropisms and also affect the oviduct and the kidneys, with serious consequences. Some strains replicate in the intestine but apparently without pathological changes. Pectoral myopathy has been associated with an important recent variant. Several factors can influence the course of infection with IBV, including the age, breed and nutrition of the chicken, the environment and intercurrent infection with other infectious agents. Immunogenic components of the virus include the S (spike) proteins and the N nucleoprotein. The humoral, local and cellular responses of the chicken to IBV are reviewed, together with genetic resistance of the chicken. In long-term persistence of IBV, the caecal tonsil or kidney have been proposed as the sites of persistence. Antigenic variation among IBV strains is related to relatively small differences in amino acid sequences in the S1 spike protein. However, antigenic studies alone do not adequately define immunological relationships between strains and cross-immunisation studies have been used to classify IBV isolates into 'protectotypes'. It has been speculated that changes in the S1 protein may be related to differences in tissue tropisms shown by different strains. Perhaps in the future, new strains of IBV may arise which affect organs or systems not normally associated with IB.

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“…IBV has a short incubation period 6 , and viral spread occurs rapidly among chickens by aerosol and mechanical means 10 , 11 . IBV has the capability to multiply in various epithelial tissues, such as trachea 12 , 13 , kidney 14 , intestine 15 , 16 , spleen 17 and oviduct 16 , 18 – 20 . The virus is well known for its effects in laying hens, including egg production and quality drops 10 , 16 , 21 , 22 .…”

Section: Introductionmentioning

confidence: 99%

Reference gene selection for gene expression study in shell gland and spleen of laying hens challenged with infectious bronchitis virus

Khan

Roberts

2017

Sci Rep

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Ten reference genes were investigated for normalisation of candidate target gene expression data in the shell gland and spleen of laying hens challenged with two strains of infectious bronchitis virus (IBV). Data were analysed with geNorm, NormFinder and BestKeeper, and a comprehensive ranking (geomean) was calculated. In the combined data set of IBV challenged shell gland samples, the comprehensive ranking showed TATA-box binding protein (TBP) and tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta (YWHAZ) as the two most stable, and succinate dehydrogenase complex flavoprotein subunit A (SDHA) and albumin (ALB) as the two least stable reference genes. In the spleen, and in the combined data set of the shell gland and spleen, the two most stable and the two least stable reference genes were TBP and YWHAZ, and ribosomal protein L4 (RPL4) and ALB, respectively. Different ranking has been due to different algorithms. Validation studies showed that the use of the two most stable reference genes produced accurate and more robust gene expression data. The two most and least stable reference genes obtained in the study, were further used for candidate target gene expression data normalisation of the shell gland and spleen under an IBV infection model.

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Susceptibility of organ cultures from chicken tissues for strains of infectious bronchitis virus isolated from the intestine

Cited by 7 publications

References 17 publications

Pathogenesis and Diagnostic Approaches of Avian Infectious Bronchitis

Pathogenesis and Diagnostic Approaches of Avian Infectious Bronchitis

Infectious bronchitis virus: Immunopathogenesis of infection in the chicken

Reference gene selection for gene expression study in shell gland and spleen of laying hens challenged with infectious bronchitis virus

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