Susceptibility of porcine cytomegalovirus to antiviral drugs

Fryer, Jacqueline F.; Griffiths, Paul; Emery, Vincent C.; Clark, Duncan A.

doi:10.1093/jac/dkh231

Cited by 28 publications

(24 citation statements)

References 17 publications

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“…As already noted, PCMV has been shown to cross the placenta in pregnant sows and to infect the fetus (70), but this model has not been explored further in the experimental study of congenital CMV infection. The study of PCMV is important for xenotransplantation, since porcine grafts are being used with increased frequency in human transplantation (87).…”

Section: Other Animal Cmvsmentioning

confidence: 99%

Neuropathogenesis of Congenital Cytomegalovirus Infection: Disease Mechanisms and Prospects for Intervention

2009

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SUMMARY Congenital cytomegalovirus (CMV) infection is the leading infectious cause of mental retardation and hearing loss in the developed world. In recent years, there has been an improved understanding of the epidemiology, pathogenesis, and long-term disabilities associated with CMV infection. In this review, current concepts regarding the pathogenesis of neurological injury caused by CMV infections acquired by the developing fetus are summarized. The pathogenesis of CMV-induced disabilities is considered in the context of the epidemiology of CMV infection in pregnant women and newborn infants, and the clinical manifestations of brain injury are reviewed. The prospects for intervention, including antiviral therapies and vaccines, are summarized. Priorities for future research are suggested to improve the understanding of this common and disabling illness of infancy.

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Section: Other Animal Cmvsmentioning

confidence: 99%

Neuropathogenesis of Congenital Cytomegalovirus Infection: Disease Mechanisms and Prospects for Intervention

2009

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“…Currently available antiviral agents have efficacy against PCMV and PLHV only at levels associated with significant toxicity in humans [3]. In our studies [4], early weaning of miniature swine after birth was capable of preventing transmission of PCMV but did not eliminate PLHV, even in combination with hysterotomy derivation and barrier rearing.…”

Section: Exogenous Agents: Herpesvirusesmentioning

confidence: 62%

Infectious risk in xenotransplantation

Mueller

Fishman

2006

Current Opinion in Organ Transplantation

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“…The pellets were processed using a DNA extraction kit (Tiangen Biotech, Beijing, China) according to the manufacturer’s recommendations. The genomic DNA pellet was resuspended in 50 μL TE buffer (pH 8.0) [30]. …”

Section: Methodsmentioning

confidence: 99%

Development and evaluation of a loop-mediated isothermal amplification assay for the rapid detection of porcine cytomegalovirus under field conditions

Yang

Zhang

Liu

et al. 2012

Virol J

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BackgroundPorcine cytomegalovirus (PCMV) induces silent infection in adult pigs but more frequently causes fatal, generalized infection in newborn piglets. This study aimed to develop a new loop-mediated isothermal amplification (LAMP) method for the sensitive, rapid, and inexpensive detection of PCMV under field conditions.MethodsTissue obtained from nine-week-old PCMV-free Landrace pigs or pig samples from postmortem examinations were analyzed. The samples were found to have clinical signs and lesions consistent with inclusion body rhinitis. Six specific primers were designed by targeting the PCMV DNA polymerase (DPOL) DNA. The LAMP reaction was optimized in a water bath. The sensitivity and specificity of LAMP and polymerase chain reaction (PCR) were compared.ResultsPCMV DNA was amplified at 65°C, and the result could be detected as early as 30 min into the reaction. Positive reactions could be visualized by the naked eye as a color change brought on by the addition of SYBR Green. The sensitivity and specificity of LAMP were found to be similar to those of the PCR.ConclusionsLAMP is a high-throughput technique for the detection of PCMV and has a high specificity, sensitivity and simplicity; these factors make it suitable for detection of PCMV under field conditions.

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Susceptibility of porcine cytomegalovirus to antiviral drugs

Abstract: These results show that, if it proves necessary, ganciclovir and cidofovir should be considered as first-line drugs to treat PCMV infections in xenograft recipients.

Cited by 28 publications

References 17 publications

Neuropathogenesis of Congenital Cytomegalovirus Infection: Disease Mechanisms and Prospects for Intervention

Neuropathogenesis of Congenital Cytomegalovirus Infection: Disease Mechanisms and Prospects for Intervention

Infectious risk in xenotransplantation

Development and evaluation of a loop-mediated isothermal amplification assay for the rapid detection of porcine cytomegalovirus under field conditions

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