Susceptibility of Prevotella intermedia/Prevotella nigrescens (and Porphyromonas gingivalis) to Propolis (Bee Glue) and other Antimicrobial Agents

Santos, Fernanda A. dos; Bastos, Esther Margarida Alves Ferreira; Rodrigues, Paulo Henrique Mazza; Uzeda, Milton de; Carvalho, Maria Auxiliadora Roque de; Farias, Luiz de Mácêdo; Moreira, E. S. A.

doi:10.1006/anae.2002.0411

Cited by 45 publications

(44 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…19 It is important to remember that these susceptibility tests are generally performed with planktonic bacterial cells. However, in periodontal pockets, microorganisms are organized in biofilms, and it is known that subgingival bacteria in biofilms are more resistant to antimicrobial treatment.…”

Section: Discussionmentioning

confidence: 99%

Metronidazole-containing gel for the treatment of periodontitis: an in vivo evaluation

Sato¹,

Fonseca²,

Ciampo

et al. 2008

Braz. oral res.

View full text Add to dashboard Cite

The aim of this investigation was to monitor metronidazole concentrations in the gingival crevicular fluid (GCF) collected from periodontal pockets of dogs after treatment with an experimental 15% metronidazole gel. Five dogs had periodontitis induced by cotton ligatures placed subgingivally and maintained for a 30-day period. After the induction period, only pockets with 4 mm or deeper received the gel. Each pocket was filled up to the gingival margin by means of a syringe with a blunt-end needle. GCF was collected in paper strips and quantified in an electronic device before and after 15 minutes, 1 h, 6 h, 24 h and 48 h of gel administration. The GCF samples were assayed for metronidazole content by means of a high performance liquid chromatography method. Concentrations of metronidazole in the GCF of the 5 dogs (mean ± SD, in µg/mL) were 0 ± 0 before gel application and 47,185.75 ± 24,874.35 after 15 minutes, 26,457.34 ± 25,516.91 after 1 h, 24.18 ± 23.11 after 6 h, 3.78 ± 3.45 after 24 h and 3.34 ± 5.54 after 48 h. A single administration of the 15% metronidazole gel released the drug in the GCF of dogs in levels several-fold higher than the minimum inhibitory concentration for some periodontopathogens grown in subgingival biofilms for up to one hour, but metronidazole could be detected in the GCF at least 48 hours after the gel application.

show abstract

Section: Discussionmentioning

confidence: 99%

Metronidazole-containing gel for the treatment of periodontitis: an in vivo evaluation

Sato¹,

Fonseca²,

Ciampo

et al. 2008

Braz. oral res.

View full text Add to dashboard Cite

show abstract

“…While 50% of the non-P. intermedia Prevotella isolates were resistant to clindamycin, all of the P. intermedia isolates were susceptible to clindamycin. Other studies indicated that 17% and 36% of the P. intermedia isolates were resistant to clindamycin (8,16).…”

mentioning

confidence: 99%

Antimicrobial Susceptibility Patterns for Recent Clinical Isolates of Anaerobic Bacteria in South Korea

Lee

Park

Kim

et al. 2010

Antimicrob Agents Chemother

View full text Add to dashboard Cite

We determined the antimicrobial susceptibilities of 255 clinical isolates of anaerobic bacteria collected in 2007 and 2008 at a tertiary-care hospital in South Korea. Piperacillin-tazobactam, cefoxitin, imipenem, and meropenem were highly active ␤-lactam agents against most of the isolates tested. The rates of resistance of Bacteroides fragilis group organisms and anaerobic Gram-positive cocci to moxifloxacin were 11 to 18% and 0 to 27%, respectively.

show abstract

“…The literature on propolis use and its many attributes in dentistry is extensive. In the oropharyngeal domain, numerous laboratory and clinical reports describe use of propolis against cariogenic organisms, 8 periodontal organisms, 9 respiratory infections, 10 gingival inflammation, 11 endodontic pathogens, 12 and oral ulcers, 13 among other oral conditions. These reports, however, lack evidence on the effectiveness of propolis because adequately designed randomized controlled trials have yet to be conducted.…”

Section: Introductionmentioning

confidence: 99%

The Effectiveness of Propolis on Gingivitis: A Randomized Controlled Trial

Bretz

Paulino

Nör

et al. 2014

The Journal of Alternative and Complementary Medicine

View full text Add to dashboard Cite

Background: A randomized, double-blind, controlled clinical trial was conducted to evaluate the effectiveness of a propolis rinse on induced gingivitis by using the co-twin study design. Methods: Twenty-one twin pairs (n = 42) were enrolled in a gingivitis study with oral hygiene promotion (14 days) and gingivitis induction (21 days). During the gingivitis induction phase, one member of the twin pair was randomly assigned to a 2% typified propolis rinse, and the other was assigned a color-matched 0.05% sodium fluoride plus 0.05% cetylpyridinium chloride rinse (positive control). Patients rinsed twice daily with 20 mL for 30 seconds for 21 days. Gingivitis was measured on days -14 (baseline), 0 (after hygiene phase), and 21 (after nohygiene phase) by using the Papillary Bleeding Score (PBS) and by standard digital imaging of the gum tissues (G-parameter). Results: The 38 persons who completed the study (age 13-22 years) were well balanced according to PBS at baseline and G-parameter after the initial hygiene phase. After 21 days without oral hygiene, the propolis rinse and positive control rinse groups did not differ significantly for average PBS measurements or G-parameter. Conclusions: Use of a 2% typified propolis rinse was equivalent to a positive control rinse during a 21-day nohygiene period.

show abstract

Susceptibility of Prevotella intermedia/Prevotella nigrescens (and Porphyromonas gingivalis) to Propolis (Bee Glue) and other Antimicrobial Agents

Cited by 45 publications

References 43 publications

Metronidazole-containing gel for the treatment of periodontitis: an in vivo evaluation

Metronidazole-containing gel for the treatment of periodontitis: an in vivo evaluation

Antimicrobial Susceptibility Patterns for Recent Clinical Isolates of Anaerobic Bacteria in South Korea

The Effectiveness of Propolis on Gingivitis: A Randomized Controlled Trial

Contact Info

Product

Resources

About