Susceptibility of Selected Multi-Drug Resistant Clinical Isolates to Different Leaf Extracts of Senna alata

Abstract

The present study evaluated the antibacterial effects of the methanolic, ethanolic and aqueous extracts of Senna alata leaves. The extracts were tested using agar well diffusion method against selected clinical isolates: Salmonella typhi, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Klebsiella pneumoniae. Antibiogram profile of the isolates deduced by disc diffusion method confirmed that the methanolic extract inhibited the growth of all tested organisms except for Klebsiella pneumoniae, which also showed no sensitivity to the ethanolic extract. There was no inhibition observed for the aqueous extract against all the tested organisms, indicating that the methanolic extract of the plant was more potent than the aqueous extract. Inhibitory activities were observed for gentamicin, ofloxacin and erythromycin against Staphylococcus aureus and Escherichia coli. No inhibitory activity was observed in all the antibiotics against Pseudomonas aeruginosa. In Klebsiella pneumoniae, inhibition was only observed in ofloxacin. The activity of both the methanolic and ethanolic extract of Senna alata was optimal under different concentrations, but gradually diminished as the concentration was adjusted. The activity of the plant extracts against the selected bacteria is an indication of the presence of broad spectrum bioactive compounds which could be explored in the therapy of bacterial infections.

Studies on the Antibacterial Susceptibility of Uropathogens to Senna alata Extracts in Calabar, Cross River State, Nigeria

“…Preliminary phytochemical analysis was carried out on the S. alata extracts to detect the presence of bioactive compounds such as: saponnins, tannins, flavonoids, alkaloids, anthraquinones, terpenoids and steroids using standard methods [55,56], with little modification.…”

Fabrication and Optimization of Electrospun Shellac Fibers Loaded with Senna alata Leaf Extract

“…The lowest loading percentage of 5% w/v ethanol extract in nanofibers showed remarkable DPPH radical scavenging, anti-tyrosinase, and antibacterial properties against S. aureus and Staphylococcus epidermidis [44]. Although numerous investigations [45][46][47][48][49] have detected noteworthy antimicrobial capabilities of SA leaf extract, no research has yet been conducted on its incorporation into electrospun nanofibers. Based on the antimicrobial properties of SA leaf extract and the unique characteristics of electrospun shellac fibers, it is possible that electrospun shellac fibers infused with this extract could promote faster wound healing.…”