Susceptibility Profile of<i>Staphylococcus epidermidis</i>and<i>Staphylococcus haemolyticus</i>Isolated from Blood Cultures to Vancomycin and Novel Antimicrobial Drugs over a Period of 12 Years

Pinheiro, Luiza; Brito, Carla Ivo; Pereira, Valéria Cataneli; Oliveira, Alexsandra Duarte de; Bartolomeu, Ariane Rocha; Camargo, Carlos Henrique; Cunha, Matheus Luís Oliveira

doi:10.1089/mdr.2015.0064

Cited by 21 publications

(9 citation statements)

References 43 publications

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“…SCC mec types have been characterized in 31 MR-CoNS isolates, as shown in Table 1, and SCC mec type II was the dominant type of S. haemolyticus (62.5%). The results of this study are similar to those of Pinheiro et al, who demonstrated the association between SCC mec type II and S. haemolyticus isolated from blood cultures [33]. In contrast, Ruppe et al demonstrated that SCC mec type V is preferentially associated with S. haemolyticus strains isolated from disparate geographical areas such as Cambodia, Algeria, Mali, and Moldova [34].…”

Section: Discussionsupporting

confidence: 89%

Molecular Characteristics of Methicillin-Resistant Staphylococci Clinical Isolates from a Tertiary Hospital in Northern Thailand

Kitti

Seng

Saiprom

et al. 2018

Canadian Journal of Infectious Diseases and Medical Microbiolog

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Methicillin-resistant staphylococci are now recognized as a major cause of infectious diseases, particularly in hospitals. Molecular epidemiology is important for prevention and control of infection, but little information is available regarding staphylococcal infections in Northern Thailand. In the present study, we examined antimicrobial susceptibility patterns, detection of antimicrobial resistance genes, and SCCmec types of methicillin-resistant S. aureus (MRSA) and methicillin-resistant coagulase-negative staphylococci (MR-CoNS) isolated from patients in a hospital in Northern Thailand. The species of MRSA and MR-CoNS were identified using combination methods, including PCR, MALDI-TOF-MS, and tuf gene sequencing. The susceptibility pattern of all isolates was determined by the disk diffusion method. Antimicrobial resistance genes, SCCmec types, and ST239 were characterized using single and multiplex PCR. ST239 was predominant in MRSA isolates (10/23). All MR-CoNS (N=31) were identified as S. haemolyticus (N=18), S. epidermidis (N=3), S. cohnii (N=3), S. capitis (N=6), and S. hominis (N=1). More than 70% of MRSA and MR-CoNS were resistant to cefoxitin, penicillin, oxacillin, erythromycin, clindamycin, gentamicin, and ciprofloxacin. In MRSA isolates, the prevalence of ermA (78.3%) and ermB (73.9%) genes was high compared to that of the ermC gene (4.3%). In contrast, ermC (87.1%) and qacA/B genes (70.9%) were predominant in MR-CoNS isolates. SCCmec type III was the dominant type of MRSA (13/23), whereas SCCmec type II was more present in S. haemolyticus (10/18). Ten MRSA isolates with SCCmec type III were ST239, which is the common type of MRSA in Asia. This finding provides useful information for a preventive health strategy directed against methicillin-resistant staphylococcal infections.

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Section: Discussionsupporting

confidence: 89%

Molecular Characteristics of Methicillin-Resistant Staphylococci Clinical Isolates from a Tertiary Hospital in Northern Thailand

Kitti

Seng

Saiprom

et al. 2018

Canadian Journal of Infectious Diseases and Medical Microbiolog

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“…Concerning the SCC mec types, it was observed that the type IV was prevalent in BSI isolates ( p = 0.007), while the type III was detected only in two of them, in contrast with the study of Pinheiro and coworkers [46] that detected 53.2% of their MRSE isolates from blood cultures carrying the SCC mec type III. However, the high frequency of the SCC mec IV in clinical isolates of S. epidermidis was already observed previously [47–49], demonstrating that this SCC mec type has become a common occurrence among hospital isolates.…”

Section: Discussioncontrasting

confidence: 58%

Methicillin resistance and virulence genes in invasive and nasal Staphylococcus epidermidis isolates from neonates

et al. 2017

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Background Staphylococcus epidermidis is an opportunistic pathogen involved in hospital-acquired infections, particularly in those related to medical devices. This study characterized 50 genetically unrelated S. epidermidis isolates from bloodstream infections (BSIs, n = 31) and nares (n = 19) of neonates in relation to staphylococcal chromosomal cassette mec (SCCmec) type, biofilm production and associated genes, and the arginine catabolic mobile elements (ACME), in order to detect virulence factors that could discriminate a potential invasiveness isolate or predict an increasing pathogenicity.ResultsIsolates from both groups showed no difference for biofilm production and ACME genes detection. However, BSI isolates harbored more frequently the sdrF and sesI genes (p < 0.05), whereas biofilm producer isolates were associated with presence of the aap gene. The sdrF gene was also significantly more in the biofilm producer isolates from BSI. The SCCmec type IV and the ccr2 complex were related to BSI isolates (p < 0.05), while 83% of the nasal isolates were non-typeable for the SCCmec elements, with the mec complex and ccr undetectable as the most frequent profile.ConclusionsDespite the great clonal diversity displayed by S. epidermidis isolates from neonates, BSI isolates harbored more frequently the sdrF and sesI adhesin genes, while nasal isolates were very variable in SCCmec composition. These aspects could be advantageous to improve colonization in the host increasing its pathogenicity.

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“…This two-stage assay was 100% in agreement with the reference method (Kloos & Schleifer (1975) and Bannerman (2003) [4]. Many investigators have employed this strategy for species-level identification of CoNS [14][15][16].…”

Section: Discussionsupporting

confidence: 65%

Accuracy of different methods for identification of Staphylococcus haemolyticus

Manoharan¹,

Sistla²,

Ray³

2021

Journal of Microbiology and Infectious Diseases

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Objectives: Staphylococcus haemolyticus is associated with device-related infections in immunocompromised individuals and acts as a reservoir for antibiotic resistance genes. It is also the species with the highest antibiotic resistance rates. However, identification is still difficult in most clinical laboratories. Simplified biochemical tests give variable results while newer methods such as MALDI-TOF MS and automated systems may not be readily available. Aim: To compare the performance of the simplified biochemical scheme, BD-Phoenix automated system, and PCR for nuc gene for the identification of S. haemolyticus with MALDI-TOF MS as the gold standard. Methods: This study included 427 coagulase-negative staphylococci (CoNS) isolates of which 356 were identified as S. haemolyticus and 71 as other species by MALDI-TOF MS. These isolates were subjected to a simplified biochemical scheme using tests like the fermentation of maltose, sucrose, trehalose, mannose, urease, xylose, ornithine, and susceptibility to novobiocin. Conventional PCR targeting the nuc gene and BD-Phoenix were also used for identification. The accuracy of these methods was assessed in comparison with MALDI-TOF MS. Results: The sensitivity and specificity of biochemical tests, BD-Phoenix and nuc PCR were 97.5% and 97.2%: 97.8% and 100%: 100% and 100% respectively. Inaccurate identification was observed for some of the isolates (2.2% by BD-Phoenix and 2.5% by biochemical tests). These isolates were identified as S. haemolyticus by the other methods. Conclusion: Identification of S. haemolyticus by biochemical tests and BD-Phoenix had good accuracy comparable to PCR as well as MALDI-TOF MS. This simplified biochemical scheme can be easily implemented even in laboratories with limited resources.

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Susceptibility Profile ofStaphylococcus epidermidisandStaphylococcus haemolyticusIsolated from Blood Cultures to Vancomycin and Novel Antimicrobial Drugs over a Period of 12 Years

Cited by 21 publications

References 43 publications

Molecular Characteristics of Methicillin-Resistant Staphylococci Clinical Isolates from a Tertiary Hospital in Northern Thailand

Molecular Characteristics of Methicillin-Resistant Staphylococci Clinical Isolates from a Tertiary Hospital in Northern Thailand

Methicillin resistance and virulence genes in invasive and nasal Staphylococcus epidermidis isolates from neonates

Accuracy of different methods for identification of Staphylococcus haemolyticus

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