Susceptibility to antimicrobials and mechanisms of erythromycin resistance in clinical isolates of Streptococcus agalactiae from Rio de Janeiro, Brazil

d'Oliveira, Rachel E.C.; Barros, Rosana Rocha; Mendonça, Cláudia R.V.; Teixeira, Lúcia Martins; Castro, Angela C. D.

doi:10.1099/jmm.0.05278-0

Cited by 13 publications

(10 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In our study ermTR was the most common macrolide resistance gene in the GBS isolates, followed by ermB. There are very few studies that report a predominance of the ermTR gene in erythromycin-resistant GBS [27]. However, the low frequency of mefA observed in this research is consistent with data obtained in other countries [4,7,8,20,25].…”

Section: Discussionsupporting

confidence: 91%

Analysis of phenotype, genotype and serotype distribution in erythromycin-resistant group B streptococci isolated from vaginal flora in Southern Ireland

Lucey

Cotter

2009

Epidemiol. Infect.

View full text Add to dashboard Cite

The screening of 2000 women of childbearing age in Cork between 2004 and 2006 produced 37 erythromycin-resistant group B streptococcus (GBS) isolates. PCR analysis was performed to determine the basis for erythromycin resistance. The ermTR gene was most frequently expressed (n = 19), followed by the ermB gene (n = 8). Four isolates harboured the mefA gene. Six isolates yielded no PCR products. Some phenotype-genotype correlation was observed. All isolates expressing the mefA gene displayed the M phenotype whilst all those expressing ermB displayed the constitutive macrolide resistance (cMLS(B)) phenotype. Of 19 isolates that expressed the ermTR gene, 16 displayed the inducible macrolide resistance (iMLS(B)) phenotype. Serotype analysis revealed that serotypes III and V predominated in these isolates. The identification of two erythromycin-resistant serotype VIII isolates among this collection represents the first reported finding of erythromycin resistance in this serotype. A single isolate was non-typable using two latex agglutination serotyping kits.

show abstract

Section: Discussionsupporting

confidence: 91%

Analysis of phenotype, genotype and serotype distribution in erythromycin-resistant group B streptococci isolated from vaginal flora in Southern Ireland

Lucey

Cotter

2009

Epidemiol. Infect.

View full text Add to dashboard Cite

show abstract

“…The high frequencies found in this study (26.8% and 26.1%) are in accordance with the literature showing average colonization ranging from 10 to 30% in different locations 23,24 . Knowledge of local profiles of antimicrobial susceptibility is of great importance because it allows the clinician to provide with more certainty the empirical treatment of chemoprophylaxis when indicated.…”

Section: Resultssupporting

confidence: 93%

Susceptibility of Streptococcus agalactiae to erythromycin and clindamycin in pregnant women in the prenatal screening

et al. 2018

View full text Add to dashboard Cite

Introduction: Group B streptococcus (GBS), or Streptococcus agalactiae, is a bacterium found in normal human microbiota. However, it may cause neonatal pneumonia, sepsis, and meningitis. Genital colonization in pregnant women is associated with a higher risk of preterm birth. The treatment of choice is antibiotic therapy with beta-lactams, but in the case of multidrug-resistance, erythromycin and clindamycin can be used. Methods:This study evaluated bacterial cultures in the period from 2014 to 2015 from a group of 29,875 pregnant women. GBS colonization and resistance to erythromycin and clindamycin were investigated.Results: Positive cultures were found in 26.8% and 26.1% of the samples in 2014 and 2015, respectively. Levels of resistance to erythromycin and clindamycin were, respectively, 2.4% and 5.5% in 2014 and 3.2% and 6.5% in 2015. Conclusion:The investigation of GBS colonization and the evaluation of GBS resistance to erythromycin and clindamycin are of extreme relevance, given the increasing incidence of bacterial resistance, risks of preterm birth, and serious consequences to newborns, representing a global health problem.

show abstract

“…15,20 A study made from 1994 to 1999 in Rio de Janeiro found in colonized women 5.4% and 1.1% of GBS resistance to erythromycin and clindamycin, respectively. 25 15 In relation to susceptibility of four pregnant women colonized by GBS in the present study, we found no resistance to the tested antimicrobials. All pregnant women were susceptible to penicillin and ampicillin, suggesting that intrapartum antibiotic prophylaxis of first choice in patients who are not allergic would have satisfactory results in our hospital.…”

Section: Discussionsupporting

confidence: 44%

<b> COLONIZATION PREVALENCE AND SUSCEPTIBILITY OF Streptococcus agalactiae IN PREGNANT WOMEN AT HUSM </b>

et al. 2011

View full text Add to dashboard Cite

The Streptococcus agalactiae colonization prevalence and its susceptibility to antimicrobials in pregnant women at University Hospital of Santa Maria (HUSM) were evaluated from June to December 2009. The vaginal-rectal material was inoculated into tubes containing Todd-Hewitt broth with subsequent subculture on blood agar. The GBS identification was made through presumptive tests, confirmed by serological test and its susceptibility was evaluated. The occurrence ofGBS maternal-fetal transmission in the colonized pregnant women was researched. The GBS colonization was 11.11%. All strains were susceptible to penicillin, ampicillin, and vancomycin. Two strains (50%) were intermediate to clindamycin and one (25%) intermediate to erythromycin. A newborn whose mother was colonized had early-onset neonatal infection by GBS. By this, it is very important the research about the colonization by GBS in all pregnant women from 35 to 37 weeks of gestation and the use of intrapartum antibiotic prophylaxis for colonized pregnant women.Descriptors: Streptococcus agalactiae; Prevalence; Pregnant women; Newborns; Microbial Sensitivity Tests. RESUMOAvaliou-se a prevalência de colonização pelo Streptococcus agalactiae e o seu perfil de sensibilidade frente aos antimicrobianos em gestantes no Hospital Universitário de Santa Maria (HUSM), de junho a dezembro de 2009. O material vaginal-retal foi inoculado em tubos contendo caldo Todd-Hewitt com posterior subcultura em ágar sangue. A identificação do EGB foi realizada através de testes presuntivos, confirmadas por teste sorológico e avaliado seu perfil de sensibilidade. Pesquisou-se ocorrência de transmissão materno-fetal do EGB nas gestantes colonizadas. A prevalência de colonização foi de 11,11%. Todas as cepas foram sensíveis à penicilina, ampicilina e vancomicina. Duas cepas (50%) foram intermediárias à clindamicina e uma (25%) intermediária à eritromicina. Um recém-nascido de mãe colonizada teve infecção neonatal de início precoce por EGB. Confirma-se a importância da pesquisa de colonização por EGB em todas as gestantes entre 35ª e 37ª semana de gestação e uso de quimioprofilaxia intraparto nas gestantes colonizadas.

show abstract

Susceptibility to antimicrobials and mechanisms of erythromycin resistance in clinical isolates of Streptococcus agalactiae from Rio de Janeiro, Brazil

Cited by 13 publications

References 9 publications

Analysis of phenotype, genotype and serotype distribution in erythromycin-resistant group B streptococci isolated from vaginal flora in Southern Ireland

Analysis of phenotype, genotype and serotype distribution in erythromycin-resistant group B streptococci isolated from vaginal flora in Southern Ireland

Susceptibility of Streptococcus agalactiae to erythromycin and clindamycin in pregnant women in the prenatal screening

<b> COLONIZATION PREVALENCE AND SUSCEPTIBILITY OF Streptococcus agalactiae IN PREGNANT WOMEN AT HUSM </b>

Contact Info

Product

Resources

About