Suspected Goat-to-Human Transmission of Methicillin-Resistant Staphylococcus aureus Sequence Type 398

Loncaric, Igor; Brunthaler, René; Spergser, Joachim

doi:10.1128/jcm.03052-12

Cited by 32 publications

(29 citation statements)

References 14 publications

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“…More recently, MRSA was detected in the nasal cavity, the vagina, and from individual dairy goat milk samples in Taiwan (Chu et al, 2012) and in milk samples obtained from clinical mastitis cases in Turkey (Aras et al, 2012), but the strains were not genotyped by multilocus sequence typing. Loncaric et al (2013) isolated MRSA from the nasal cavity and reported lung infection in a goat, also suspecting a possible transmission of the same MRSA strain to farmers.…”

Section: Short Communicationmentioning

confidence: 99%

Short communication: Prevalence of Staphylococcus aureus and methicillin-resistant S. aureus in bulk tank milk from dairy goat farms in Northern Italy

Cortimiglia

Bianchini

Franco

et al. 2015

Journal of Dairy Science

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Staphylococcus aureus is regarded as a leading cause of mastitis in goats. However, few data are available on the presence of methicillin-resistant S. aureus (MRSA) in this species. The aim of this study was to assess the prevalence of S. aureus and MRSA in bulk tank milk samples from dairy goat farms in Northern Italy. Eighty-five out of 197 samples (43.1%) tested positive for S. aureus with counts ranging from 10 to more than 1.5 × 10(4) cfu/mL. The MRSA was screened by both direct plating followed by a disk diffusion test to evaluate methicillin resistance and a selective enrichment method. Methicillin-resistance was confirmed by mecA-specific PCR. Methicillin-resistant S. aureus was identified in 4 samples (2.0%) and multilocus sequence typing (MLST) showed the presence of livestock-associated MRSA belonging to lineages ST398 (n = 3) and ST1 (n = 1). In one case we demonstrated that the same MRSA strain was able to persist over time on the farm, being isolated from both bulk tank milk and the udder of 3 goats 1 yr after the first isolation. The high prevalence of S. aureus-positive herds detected in this study and the presence of MRSA strains belonging to livestock-associated genotypes is of concern, and represents a novel finding in the Italian dairy goat production system. The application of stringent measures for the control of S. aureus mastitis at the farm level seems appropriate to reduce the economic losses, and to minimize the risk of foodborne illness and the transmission of MRSA to humans by occupational exposure.

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Section: Short Communicationmentioning

confidence: 99%

Short communication: Prevalence of Staphylococcus aureus and methicillin-resistant S. aureus in bulk tank milk from dairy goat farms in Northern Italy

Cortimiglia

Bianchini

Franco

et al. 2015

Journal of Dairy Science

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“…Nasal swabs of clinically healthy cattle (n = 55) and sheep (n = 38, 10 from organic farms) were cultured for MRSA; selected colonies were tested for cefoxitin resistance and confirmed as S. aureus by PCR. 7,8 Eight S. aureus isolates, originating from four different cattle and three sheep, were resistant to cefoxitin. Isolates were tested for mecA and mecC, and SCCmec were typed.…”

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confidence: 99%

“…Isolates were tested for mecA and mecC, and SCCmec were typed. 5,8 All MRSA isolates were further investigated for PVL genes, spa typing, two multiple-locus, variable-number tandem repeat analysis (MLVA) and multilocus sequence typing (MLST). 8 Amplicons (including all mecC) were sequenced by LGC Genomics (Berlin, Germany).…”

mentioning

confidence: 99%

mecC‐ and mecA‐positive meticillin‐resistant Staphylococcus aureus (MRSA) isolated from livestock sharing habitat with wildlife previously tested positive for mecC‐positive MRSA

Loncaric

Kübber‐Heiss

Posautz

et al. 2014

Veterinary Dermatology

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“…Nineteen genes, except seq, were detected in this study. The most prevalent gene was sei (52, 58.4%), followed by tst-1 ( (19,21.3%). The prevalence rates of 5 genes were between 10% and 20%, namely, seh (13, 14.6%), ser (12, 13.5%), ses (11, 12.4%), set (10, 11.2%)and sed (9, 10.1%).…”

Section: Profile Of Staphylococcal Se/sel/tsst-1 Genesmentioning

confidence: 99%

“…This indicated that pigs were important reservoir for human-infected MRSA. Recent studies also found that MRSA ST398 could be isolated from other animals, such as goats [19], rabbits [20], and cattle [21], etc. In addition, methicillin-susceptible S. aureus (MSSA) isolated from both human and animals also exhibited multi-drug resistance (MDR) to various classes of antimicrobials [22,23].…”

Section: Introductionmentioning

confidence: 98%

Prevalence, antimicrobial resistance and staphylococcal toxin gene of blaTEM-1a-producing Staphylococcus aureus isolated from animals in Chongqing, China

Wang

Yao

Jia

et al. 2019

Preprint

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Background Livestock-associated Staphylococcus aureus is one of the most important etiological agents in both human and animals. It has been reported with high antimicrobial resistance and multiple staphylococcal superantigen genes in many countries and several provinces of China. However, large-scale investigation of this organism has not been documented in Chongqing, China. The aim of this study is to demonstrate the prevalence, antimicrobial susceptibility and some molecular characteristics of S. aureus acquired from animals in Chongqing. Results A total of 89 S. aureus isolates were cultured from 1371 samples picked up from March 2014 to December 2017. The isolates were originated from pigs (25), cattle (6), goats (10), rabbits (16) and chicken (32). Four MRSA strains were identified from 3 pig samples and 1 chicken sample. The isolates showed high resistance to penicillin (93.3%) and ampicillin (92.1%), but were more susceptible to amikacin and ofloxacin, since the resistance rates of these two drugs were less than 10%. Meanwhile, 74.2% isolates exhibited varying degree of MDR. Almost all strains, except for 3 chicken-originated isolates, were positive for blaTEM-1a, but did not harbor other ESBL genes. Nineteen staphylococcal SE/SEl/TSST-1 genes, except seq, were detected in isolates. The predominant genes were sei (58.4%), tst-1 (56.2%) and seg (51.7%). Conclusions The high antimicrobial resistance and prevalence of blaTEM-1a seriously reminded that it was urgent to standardize and cut down the usage of antimicrobials. The universal existence of staphylococcal toxin genes in isolated strains implied a potential threat of public health from animals to human through the food chain.

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Suspected Goat-to-Human Transmission of Methicillin-Resistant Staphylococcus aureus Sequence Type 398

Cited by 32 publications

References 14 publications

Short communication: Prevalence of Staphylococcus aureus and methicillin-resistant S. aureus in bulk tank milk from dairy goat farms in Northern Italy

Short communication: Prevalence of Staphylococcus aureus and methicillin-resistant S. aureus in bulk tank milk from dairy goat farms in Northern Italy

mecC‐ and mecA‐positive meticillin‐resistant Staphylococcus aureus (MRSA) isolated from livestock sharing habitat with wildlife previously tested positive for mecC‐positive MRSA

Prevalence, antimicrobial resistance and staphylococcal toxin gene of blaTEM-1a-producing Staphylococcus aureus isolated from animals in Chongqing, China

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