Suspended crystalline films of protein hydrophobin I (HFBI)

Vrielink, Anneloes S. Oude; Bomans, Paul H. H.; Vredenbregt, E.J.D.; Wirix, Maarten; Sommerdijk, Nico A. J. M.; Luiten, O.J.; Voets, Ilja K.

doi:10.1016/j.jcis.2015.01.073

Cited by 8 publications

(5 citation statements)

References 29 publications

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“…More recently, Chen et al developed a codisplay system in which PETase and hydrophobin are simultaneously displayed on the yeast surface. Hydrophobins (HFBs) have been demonstrated to increase the attachment of several enzymes onto different substrates in vitro . − Class II hydrophobin I from Trichoderma reesei (HFBI) was used in these experiments.…”

Section: Petase and Mhetase: A Variety Of Ways For Their Applicationsmentioning

confidence: 99%

Development of Enzyme-Based Approaches for Recycling PET on an Industrial Scale

Oda,

Wlodawer

2024

Biochemistry

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Pollution by plastics such as polyethylene (PE), polypropylene (PP), polyvinyl chloride (PVC), polyurethane (PUR), polyamide (PA), polystyrene (PS), and poly(ethylene terephthalate) (PET) is now gaining worldwide attention as a critical environmental issue, closely linked to climate change. Among them, PET is particularly prone to hydrolysis, breaking down into its constituents, ethylene glycol (EG) and terephthalate (TPA). Biorecycling or bioupcycling stands out as one of the most promising methods for addressing PET pollution. For dealing with pollution by the macrosize PET, a French company Carbios has developed a pilot-scale plant for biorecycling waste PET beverage bottles into new bottles using derivatives of thermophilic leaf compost cutinase (LCC). However, this system still provides significant challenges in its practical implementation. For the micro-or nanosize PET pollution that poses significant human health risks, including cancer, no industrial-scale approach has been established so far, despite the need to develop such technologies. In this Perspective, we explore the enhancement of the low activity and thermostability of the enzyme PETase to match that of LCC, along with the potential application of microbes and enzymes for the treatment of waste PET as microplastics. Additionally, we discuss the shortcomings of the current biorecycling protocols from a life cycle assessment perspective, covering aspects such as the diversity of PET-hydrolyzing enzymes in nature, the catalytic mechanism for crystallized PET, and more. We also provide an overview of the Ideonella sakaiensis system, highlighting its ability to operate and grow at moderate temperatures, in contrast to hightemperature processes.

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Section: Petase and Mhetase: A Variety Of Ways For Their Applicationsmentioning

confidence: 99%

Development of Enzyme-Based Approaches for Recycling PET on an Industrial Scale

Oda,

Wlodawer

2024

Biochemistry

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“…Other applications have been explored in drug nanosuspensions, drug delivery [28,29] and tissue engineering [30,31]. Recently, the applications of HFBs were broadened to include the modification of the cryo-electron microscopy support film [32,33]. Being an industrially important protein, identification and heterologous expression of new HFB molecules are urgently needed.…”

Section: Introductionmentioning

confidence: 99%

Identification and Characterization of a Predominant Hydrophobin in the Edible Mushroom Grifola frondosa

Song,

Wang,

Jia

et al. 2023

JoF

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Hydrophobins (HFBs) are a group of small, secreted amphipathic proteins of fungi with multiple physiological functions and potential commercial applications. In this study, HFB genes of the edible mushroom, Grifola frondosa, were systematically identified and characterized, and their transcriptional profiles during fungal development were determined. In total, 19 typical class I HFB genes were discovered and bioinformatically analyzed. Gene expression profile examination showed that Gf.hyd9954 was particularly highly upregulated during primordia formation, suggesting its major role as the predominant HFB in the lifecycle of G. frondosa. The wettability alteration profile and the surface modification ability of recombinant rGf.hyd9954 were greater than for the Grifola HFB HGFII-his. rGf.hyd9954 was also demonstrated to form the typical class I HFB characteristic-rodlet bundles. In addition, rGf.hyd9954 was shown to possess nanoparticle characteristics and emulsification activities. This research sheds light on the regulation of fungal development and its association with the expression of HFB genes.

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“…In particular, systems combining globular and disordered flexible proteins have been studied broadly [29,30]. Globular proteins are typically described as more rigid and require denaturation to adsorb at hydrophobic interfaces [17,30,31], or at least conformational changes underpinning surface adsorption and selfassociation [32][33][34]. In turn, disordered proteins such as casein can adsorb faster at interfaces, owing to their softer, less structured architecture [31,35].…”

Section: Introductionmentioning

confidence: 99%

Interfacial Mechanics of b-Casein and Albumin Mixed Protein Assemblies at Liquid-Liquid Interfaces

Chrysanthou,

Bosch-Fortea,

Zarbakhsh

et al. 2023

Preprint

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Protein emulsifiers play an important role in formulation science, from food product development to emerging applications in biotechnologies. The impact of mixed protein assemblies on surface composition and interfacial shear mechanics remains broadly unexplored, in comparison to the impact that formulation has on dilatational mechanics and surface tension or pressure. In this report, we use interfacial shear rheology to quantify the evolution of interfacial shear moduli as a function of composition in bovine serum albumin (BSA)/b-casein mixed assemblies. We present the striking difference in mechanics of these two protein and observe the dominance of -casein in regulating interfacial shear mechanics. This observation correlates well with the strong asymmetry of adsorption of these two proteins, characterised by fluorescence microscopy. Using neutron reflectometry and fluorescence recovery after photobleaching, we examine the architecture of corresponding protein assemblies and their surface diffusion, providing evidence for distinct morphologies, but surprisingly comparable diffusion profiles. Finally, we explore the impact of crosslinking and sequential protein adsorption on the interfacial shear mechanics of corresponding assemblies. Overall, this work indicates that, despite comparable surface densities, BSA and b-casein assemblies at liquid-liquid interfaces display almost 2 orders of magnitude difference in interfacial shear storage modulus and strikingly different viscoelastic profiles. In addition, co-adsorption and sequential adsorption processes are found to further modulate interfacial shear mechanics. Beyond formulation science, the understanding of complex mixed protein assemblies and mechanics may have implications for the stability of emulsions and may underpin changes in the mechanical strength of corresponding interfaces, for example in tissue culture or physiological conditions.

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Suspended crystalline films of protein hydrophobin I (HFBI)

Cited by 8 publications

References 29 publications

Development of Enzyme-Based Approaches for Recycling PET on an Industrial Scale

Development of Enzyme-Based Approaches for Recycling PET on an Industrial Scale

Identification and Characterization of a Predominant Hydrophobin in the Edible Mushroom Grifola frondosa

Interfacial Mechanics of b-Casein and Albumin Mixed Protein Assemblies at Liquid-Liquid Interfaces

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