Suspension culture promotes serosal mesothelial development in human intestinal organoids

Suspension culture in a rotating bioreactor for efficient generation of human intestinal organoids

“… 17 The other reports suspension culture of HIOs; however, the induction of hindgut spheroids, which is the most complicated and variable part, follows the conventional method. 30 Our study demonstrates effective suspension culture throughout the entire culture process from hindgut spheroid induction to HIO maturation.…”

Aggregation of cryopreserved mid-hindgut endoderm for more reliable and reproducible hPSC-derived small intestinal organoid generation

“…Distribution of cells from these banks will also greatly enhance and facilitate successful dissemination of HIO technologies by eliminating the need for receiving laboratories to be trained in hPSC culture and differentiation to DE and MHE. Furthermore, in combination with approaches that facilitate HIO production from spheroids in suspension culture ( Capeling et al., 2022 ), generation of MHE spheroids by aggregation may significantly enhance translational efforts by both increasing scalability and reducing hands-on labor requirements. Finally, we show that aggregation can be applied to the production of colonic and antral stomach organoids, indicating that additional GI organoid types can be reliably produced by this method.…”

Growth factor dependency in mammary organoids regulates ductal morphogenesis during organ regeneration

“…Although, Matrigel has been utilized for decades it often shows variable protein concentrations and batch-to-batch variability. Matrigel being an animal-derived product impose bottleneck to bulk production and its ill-defined composition inspire the development of synthetic scaffold alternatives 40 or generate organoids as suspension culture 55 . In this study we developed a suspension culture method using minimal concentration of Matrigel that provides the extracellular support to generate 3D organoids.…”