Suspension-Vero cell cultures as a platform for viral vaccine production

Paillet, Cristian; Forno, Guillermina; Kratje, Ricardo; Etcheverrigaray, Marina

doi:10.1016/j.vaccine.2009.06.020

Cited by 43 publications

(25 citation statements)

References 11 publications

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“…The reduced temperature of 33°C did not have an effect on the optimal trypsin concentration due to an only slightly impaired enzyme activity at this temperature [34]. However, a design of experiment approach considering trypsin activity and other parameters would be useful for further process optimization [17]. Shaker flask experiments were found to be highly predictive and scale-up to stirred tank bioreactors was possible without further optimization and without loss of productivity.…”

Section: Discussionmentioning

confidence: 99%

Live attenuated influenza viruses produced in a suspension process with avian AGE1.CR.pIX cells

Lohr

Genzel

Jordan³

et al. 2012

BMC Biotechnol

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BackgroundCurrent influenza vaccines are trivalent or quadrivalent inactivated split or subunit vaccines administered intramuscularly, or live attenuated influenza vaccines (LAIV) adapted to replicate at temperatures below body temperature and administered intranasally. Both vaccines are considered safe and efficient, but due to differences in specific properties may complement each other to ensure reliable vaccine coverage. By now, licensed LAIV are produced in embryonated chicken eggs. In the near future influenza vaccines for human use will also be available from adherent MDCK or Vero cell cultures, but a scalable suspension process may facilitate production and supply with vaccines.ResultsWe evaluated the production of cold-adapted human influenza virus strains in the duck suspension cell line AGE1.CR.pIX using a chemically-defined medium. One cold-adapted A (H1N1) and one cold-adapted B virus strain was tested, as well as the reference strain A/PR/8/34 (H1N1). It is shown that a medium exchange is not required for infection and that maximum virus titers are obtained for 1 × 10-6 trypsin units per cell. 1 L bioreactor cultivations showed that 4 × 106 cells/mL can be infected without a cell density effect achieving titers of 1 × 108 virions/mL after 24 h.ConclusionsOverall, this study demonstrates that AGE1.CR.pIX cells support replication of LAIV strains in a chemically-defined medium using a simple process without medium exchanges. Moreover, the process is fast with peak titers obtained 24 h post infection and easily scalable to industrial volumes as neither microcarriers nor medium replacements are required.

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Section: Discussionmentioning

confidence: 99%

Live attenuated influenza viruses produced in a suspension process with avian AGE1.CR.pIX cells

Lohr

Genzel

Jordan³

et al. 2012

BMC Biotechnol

View full text Add to dashboard Cite

show abstract

“…The majority of proteomic studies were applied to study the proteome profile of the Vero cell line, because World Health Organization (WHO) strongly recommended this cell line for systemic research and for production of live vaccine against influenza [142,143], WNV, vesicular stomatitis virus, herpes simplex virus 1 and polio virus 1 [144], rabies virus [145], Japanese encephalitis virus or reovirus [146] and others.…”

Section: Choice Of Cell Line For Vaccine Productionmentioning

confidence: 99%

Proteomics for development of vaccine

Adamczyk-Popławska

Markowicz

Jagusztyn-Krynicka

2011

Journal of Proteomics

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“…Although during the early phase of culture, lower cell number and lower specific growth rate were observed, after 50 days, the total cell number and cell growth rate increased. In a previous study, Vero cells adapted to suspension growth showed a 1.5-fold efficacy for VSV propagation [14]. Another study demonstrated that suspension-adapted Vero cells produced 3-fold higher amounts of viruses than adherent Vero cells [7].…”

Section: Discussionmentioning

confidence: 90%