Sustainable biodegradation of textile dye reactive blue 222 by the novel strain Enterobacter CU2004, isolated from the industrial waste: A design of experiment based optimization study and characterisation of metabolites

Abstract

Reactive Blue 222 (RB222) is widely used in textile industries and hence a common recalcitrant pollutant in the industrial effluent. Bioremediation of this dye is of significance as its one of the complex dyes with high molecular weight. In the present study, we isolated a novel bacterial strain Enterobacter CU2004 from the industrial waste and characterize using16S rRNA gene sequencing. Its potential to dye degradation was evaluated in a simple minimal salt media with the parameters namely dye concentration (100-1000 ppm), pH (4-9), temperature (15-55°C), Carbon source (Lactose, Sucrose, Glucose, Starch, and Fructose), and Nitrogen source (Casein, Yeast extract, Peptone, Tryptone, Ammonium sulphate, and Urea) in a 24 h culture. Finally, data obtained were extended to design of experiment based optimization for the degradation efficacy of Enterobacter CU2004 and to validated design space was established. The novelty is in optimizing the design space parameters for highest percentage of degradation ≥90% by the bacterial isolate Enterobacter CU2004 were finalized as 30-37°C temperature, 133-249 ppm dye concentration, Lactose as Carbon source, Yeast extract as Nitrogen source, and the pH as 8. Microbial dye degradation was confirmed by FTIR, HPLC and GCMS studies. Further studies revealed the dye intermediates and the potential of Enterobacter CU2004 toward the degradation of complex, high molecular weight industrial dye RB222.

Exploring the decolorization efficiency and biodegradation mechanisms of different functional textile azo dyes by Streptomyces albidoflavus 3MGH

“…Previous studies highlighted the effect of temperature on decolorization rate and how temperature can affect the microbe’s physiology [ 85 , 87 – 90 ]. Our results are in harmony with those of Khan and Malik, who also observed an increased bio-decolorization rate of RB5 dye in the mesophilic temperature range [ 91 , 92 ]. Endo (2003) reported that at 40 °C, Streptomyces sp.…”

“…While, Vinayak and Singh [ 39 ] showed that the best carbon source was glucose, followed by sucrose and fructose, in the bio-decolorization of Evans blue azo dye using four different Streptomyces spp. In contrast, Lakshmaiah et al [ 92 ] reported lactose to be the best carbon source and yeast extract to be the best nitrogen source for the bio-decolorization of reactive blue 222 azo dye by Kucoria marina CU2005.…”

Decolorization of Distillery Effluent by the Novel Bacterial Strain Bacillus nitratireducens (B2)

“…This isolate is a viable candidate for bioremediation because of its expansive potential in building a cost-effective, efficient, and scalable microbial dye-degrading process. 40 Over the past few decades, the use of microorganisms for bioremediation has gained increasing attention. Multiple microorganisms, including bacteria and fungi, exhibit a promising ability to decolorize melanoidincontaining wastewater from the distillation industry.…”