The new renewable energy source, namely microalgae biomass, which is the third generation in its utilization derivative, has a lipid content productivity that has great potential to be used as raw material for biodiesel production. One species of microalgae, Nannochloropsis sp., is ideal for cultivation and has a higher photosynthetic efficiency than land plants. Methods to obtain microalgae oil from dry raw materials can be carried out from several processes, but the most effective and easy method is extraction with solvents using a Soxhlet device. The purpose of this study was to determine the optimal growth of Nannochloropsis sp microalgae cell density observations in the cultivation process and to see the percent yield of lipids from microalgae Nannochloropsis sp. through the extraction process with the Soxhlet method using n-hexane and ethanol solvents at a ratio of 1:1, 1:2, 1:3, 1:4 and 1:5. The optimal amount of cell density during the cultivation process has obtained an average of 32.206 x 104 cells/ml on day of 9th, and the optimal lipid yield was in the ratio of hexane: ethanol 1:1 with a value of 32.13%. These results show that the optimal conditions for yield can be obtained and also has the potential as a raw material biodiesel production