Sustainable biosurfactant produced by Serratia marcescens UCP 1549 and its suitability for agricultural and marine bioremediation applications

Araújo, Hélvia Walewska Casullo de; Andrade, Rosileide F. S.; Rodríguez, Dayana Montero; Ribeaux, Daylin Rubio; Silva, Carlos Alberto Alves da; Campos-Takaki, Galba Maria de

doi:10.1186/s12934-018-1046-0

Cited by 105 publications

(55 citation statements)

References 69 publications

(107 reference statements)

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“…Preliminary biochemical characterization showed that the bioemulsifier had a polymeric structure, composed of lipids (37.6%), proteins (28.2%), and carbohydrates (14.7%). Similarly, Araújo et al [48] carried out these methodologies to obtaining the biochemical composition of biosurfactant produced by Serratia marcescens UCP 1549 and revealed the presence of 43% lipids, 32% proteins, and 11% carbohydrates, suggesting its polymeric nature. e biosurfactant obtained by Stenotrophomonas sp.…”

Section: Characterization Of Bioemulsifiermentioning

confidence: 99%

Bioconversion of Agroindustrial Waste in the Production of Bioemulsifier by Stenotrophomonas maltophilia UCP 1601 and Application in Bioremediation Process

Nogueira

Rodríguez

Andradade

et al. 2020

International Journal of Chemical Engineering

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This study investigated the potential of the bacterium Stenotrophomonas maltophilia UCP 1601 to produce a new biomolecule with emulsifying properties by determining the hemolytic activity, obtaining a halo of 9 mm in blood agar. Fermentations were carried out in saline mineral medium supplemented with 10% waste soybean oil (WSO) and different concentrations of glucose, peptone, ZnCl2, and MgSO4, according to a 24 full-factorial design. The results showed that the best results were obtained in condition 6 (medium composed of 4% glucose, 1% peptone, 2.72% ZnCl2, and 2.46% MgSO4), with excellent high emulsification index of 82.74%, using burned motor oil. The emulsifying property of the biomolecule produced was confirmed by the emulsification index of 78.57, 54.07, and 58.62%, using soybean, corn, and diesel oils, respectively, and the stability at different values of pH, temperature, and NaCl concentrations. The yield of the produced bioemulsifier was 2.8 g/L, presenting an anionic character and polymeric nature (37.6% lipids, 28.2% proteins, and 14.7% carbohydrates), confirmed by FTIR. The new bioemulsifier demonstrated promising potential for bioremediation of hydrophobic contaminants in the environment, since it had the ability to reduce the viscosity of WSO and burned motor oil, as well as excellent dispersion capacity of the burned motor oil in water (69.94 cm2 of oil displacement area), and removing 71.7% of this petroleum derivative from sandy soil.

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Section: Characterization Of Bioemulsifiermentioning

confidence: 99%

Bioconversion of Agroindustrial Waste in the Production of Bioemulsifier by Stenotrophomonas maltophilia UCP 1601 and Application in Bioremediation Process

Nogueira

Rodríguez

Andradade

et al. 2020

International Journal of Chemical Engineering

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“…PG was first isolated in the 1920s, its structural features were identified in the 1930s, and structure elucidation was completed in the 1960s [ 163 , 164 , 165 , 166 ]. Serratia marcescens is the major source of PG production [ 59 , 60 , 61 , 62 , 81 , 82 , 83 , 84 , 85 , 86 , 87 , 88 , 89 , 90 , 91 , 92 , 93 , 94 , 95 , 96 , 97 , 98 , 99 , 100 , 101 , 102 , 103 , 104 , 105 , 106 , 107 , 108 , 109 , 110 , 111 , 112 , 113 , 114 , 115 , 116 , 117 , 118 , 119 , 120 , 121 , 122 , 123 , 124 , 125 , …”

Section: Introductionmentioning

confidence: 99%

“…Some PG-related reviews reported the production of PG from S. marcescens using commercial media such as nutrient broth, peptone glycerol broth, and seed oils as the C/N sources [ 156 , 161 , 162 , 166 , 168 , 171 ]. The biological potentials of PG especially in the field of anticancer have also been reviewed [ 156 , 157 , 158 , 159 , 160 , 161 , 162 , 166 , 168 , 169 , 171 ]. For example, a review of the characteristics and potential therapeutic anticancer-drug applications of PG from Serratia was introduced by Darshan and Manonmani [ 180 ], while the structure, chemical synthesis, and biosynthesis of PGs as natural products were summarized by Hu et al [ 157 ].…”

Section: Introductionmentioning

confidence: 99%

Production and Potential Applications of Bioconversion of Chitin and Protein-Containing Fishery Byproducts into Prodigiosin: A Review

et al. 2020

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The technology of microbial conversion provides a potential way to exploit compounds of biotechnological potential. The red pigment prodigiosin (PG) and other PG-like pigments from bacteria, majorly from Serratia marcescens, have been reported as bioactive secondary metabolites that can be used in the broad fields of agriculture, fine chemicals, and pharmacy. Increasing PG productivity by investigating the culture conditions especially the inexpensive carbon and nitrogen (C/N) sources has become an important factor for large-scale production. Investigations into the bioactivities and applications of PG and its related compounds have also been given increased attention. To save production cost, chitin and protein-containing fishery byproducts have recently been investigated as the sole C/N source for the production of PG and chitinolytic/proteolytic enzymes. This strategy provides an environmentally-friendly selection using inexpensive C/N sources to produce a high yield of PG together with chitinolytic and proteolytic enzymes by S. marcescens. The review article will provide effective references for production, bioactivity, and application of S. marcescens PG in various fields such as biocontrol agents and potential pharmaceutical drugs.

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“…This is include P. aeruginosa [34, 35], Salmonella enteridis [36], Acinetobacter sp. [37], and Serratia Marcescens [38]. Gram positive bacteria are known as professional one in the production of BLM.…”

Section: Discussionmentioning

confidence: 99%

Invasion of epithelial cells are correlated with secretion of Biosurfactant via the type 3 secretion system (SST3) ofShigella flexneri

Kinavouidi

Kayath²,

Nguimbi

2020

Preprint

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11Biosurfactants are amphipathic molecules produced by many microorganisms, usually bacteria, 12 fungi and yeasts. They possess the property of reducing the tension of the membrane interfaces. 13 No studies have been conducted on Shigella species showing their involvement of biosurfactant 14 like molecules (BLM) in pathogenicity. This study aims to show that environmental and clinical 15 strains of Shigella are able to produce BLM by emulsifying gasoline and diesel fuels. Our study 16 has shown that BLM are secreted in the extracellular medium with EI24 ranging from 80 to 17 100%. The secretion is depending on the type III secretion system (T3SS). We did show that S. 18 flexneri, S. boydii and S. sonnei are able to interact with hydrophobic areas with respectively 19 17.64%, 21.42% and 22.22% of hydrophobicity. 100 mM Benzoic and 1.5mg/mL Salycilic 20 acids have been inhibited T3SS and this totally stops the BLM secretion. Pseudomonas 21 aeruginosa which has T3SS is able to produce 100% of BLM in the presence or in the absence 22 of both T3SS inhibitors. The secreted BLM is extractable with an organic solvent such as 23 chloroform and could entirely be considered like lipopeptide or polypeptidic compound. By 24 secreting BLM, Shigella is able to perform multicellular phenomena like "swarming" allowing 25 to invade and disseminate inside epithelial cells. 26 Introduction 28 The ingestion of pathogenic and virulent microorganisms generally affecting peoples in both 29 developed and developing countries [1]. Shigella is one of the Gram-negative bacterium 30 belonging to Enterobacteriaceae family and is causative agent of bacillary dysentery or 31 shigellosis [2]. The genus Shigella was the major pathogen bacteria associated with dysentery 32with attributable fraction to 63,8%, but also the second most common pathogen associated with 33 watery diarrhoea with attributable fraction to 12,9% in sub-Saharan Africa and south Asia. 34 Children under 5 years are the most affected. More and more shigellosis is a pathology that 35 both towards neglected diseases but 164300 of death per years have been notified all over the 36 world in 2010. Most deaths occur in sub-Saharan Africa and in south Asia [3-6]. This is include 37 Republic of Congo and surprisingly no epidemiological studies have been conducted in this 38 field. The genus Shigella includes four species (S. flexneri, S. sonnei, S. dysenteriae and S. 39 boydii) [7]. 10 bacteria of S. dysenteriae type 1 and 100 to 180 bacteria of S. flexneri or S. 40 sonnei are enough to produce symptomatic infection [8].41Shigella's pathogenicity is based on a virulence plasmid pWR100 in which the mxi-spa locus 42 encodes the type three secretion system(T3SS) involved in effector production like IpaB, C and 43 D (Translocator and Tip) to invade host cell [9][10][11][12]. A previous study in our laboratory that 44 showed that Shigella sp. isolated from Brazzaville wastewater were able to emulsify 45 hydrocarbon from gasoline and/or diesel fuel [13]. Sachin et al. found the same pr...

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Sustainable biosurfactant produced by Serratia marcescens UCP 1549 and its suitability for agricultural and marine bioremediation applications

Cited by 105 publications

References 69 publications

Bioconversion of Agroindustrial Waste in the Production of Bioemulsifier by Stenotrophomonas maltophilia UCP 1601 and Application in Bioremediation Process

Bioconversion of Agroindustrial Waste in the Production of Bioemulsifier by Stenotrophomonas maltophilia UCP 1601 and Application in Bioremediation Process

Production and Potential Applications of Bioconversion of Chitin and Protein-Containing Fishery Byproducts into Prodigiosin: A Review

Invasion of epithelial cells are correlated with secretion of Biosurfactant via the type 3 secretion system (SST3) ofShigella flexneri

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