In the existing study, the performance of (I2/I3 -) as the efficient redox catholyte for escherchia coli based microbial fuel cell (MFC) was investigated and compared with conventional ferricyanide based system. This studies also examined the effect of applied current density, linear sweep voltammetry, open circuit voltage, discharge capacitance, Impedance, Energy and power density of the microbial fuel cell. Linear sweep voltammetry studies revealed the higher current density (3.3768 mAcm -2 ) for(I2/I3 -)additive owing to its electro-reduction and this value is about 1.5 times higher in comparison to ferricyanide based system.The characteristics of (I2/I3 -)based MFC is attributed to its higher capacitive contribution, high conductivity, and high power, when compared to ferricyanide based MFC. The extreme power density of the MFC fabricated using (I2/I3 -)and ferricyanide additive at the measured current density of 50 µAcm -2 were observed to be 1,140 and 1,670.8303mWm -2 . Among the results, (I2/I3 -) basedMFCperformance displays excessive potential for enlightening the power generation of MFC.