Sustainable Development Goals

Seyedsayamdost, Elham

doi:10.4324/9780367816681-102

Cited by 13 publications

(17 citation statements)

References 1 publication

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“…23 Detailed analysis of the NMR J-coupling patterns in conjunction with previous work on the related actinobolin (see below) established equatorial hydroxy groups at C-5 and C-6. 21,24 Moreover, the NMR data allowed discrimination of axial-axial and axial-equatorial relationships between other protons in the bicycle and together with Marfey's and Mosher's ester analysis completed the full configurational assignment of bactobolin A. The data were consistent with a half-chair conformation within the carbocycle, a proposal later confirmed by the X-ray crystal structure of bactobolin A (see below).…”

Section: Bactobolin Isolation and Characterizationmentioning

confidence: 70%

“…The mutants and growth conditions established in the Greenberg lab helped identify the antibiotic, which was ultimately characterized as bactobolin. 21…”

Section: Initial Discovery Of Bactobolinmentioning

confidence: 99%

“…Initial isolations utilized a five-column purification scheme, which was successful because of the robust production and stability of the antibiotic. 21 The purification protocol was later simplified with increased understanding of the chromatographic and chemical properties of bactobolin. 22 Activity-guided fractionation enabled purification of four variants.…”

Section: Bactobolin Isolation and Characterizationmentioning

confidence: 99%

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The Chemistry and Biology of Bactobolin: A 10-Year Collaboration with Natural Product Chemist Extraordinaire Jon Clardy

2020

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Bactobolin is a hybrid natural product with potent cytotoxic activity. Its production from Burkholderia thailandensis was reported as part of a collaboration between the Greenberg and Clardy laboratories in 2010. The collaboration sparked a series of studies leading to the discovery of new analogues and associated structure-activity relationships, the identification of the bactobolin biosynthetic gene cluster and assembly of its unusual amino acid building block, the molecular target of and resistance to the antibiotic, and finally an X-ray crystal structure of the ribosome-bactobolin complex. Herein, we review the collaborations that led to our current understanding of the chemistry and biology of bactobolin.

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Section: Bactobolin Isolation and Characterizationmentioning

confidence: 70%

“…The mutants and growth conditions established in the Greenberg lab helped identify the antibiotic, which was ultimately characterized as bactobolin. 21…”

Section: Initial Discovery Of Bactobolinmentioning

confidence: 99%

Section: Bactobolin Isolation and Characterizationmentioning

confidence: 99%

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The Chemistry and Biology of Bactobolin: A 10-Year Collaboration with Natural Product Chemist Extraordinaire Jon Clardy

2020

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“…Sustainable development goal (SDG) 7 focuses on clean and sustainable energy sources to meet the world's energy demands. 36 Numerous alternative energy resources are available, but solar energy is pondered as the most suitable choice for fulfilling our energy needs because the enormous energy content of sunlight (1000 W/m 2 ) is available on the earth daily. 37,38 Therefore, solar cells were utilized in this research as a replacement for conventional DC power sources because they can deliver adequate power for MEC operation.…”

mentioning

confidence: 99%

Evaluating the use of unassimilated bio‐anode with different exposed surface areas for bioenergy production using solar‐powered microbial electrolysis cell

et al. 2021

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The microbial electrolysis cell (MEC) is an emerging technology for bioenergy production using organic wastewater. Normally, a preassimilated bio-anode is utilized by the MEC to break down the organic content, but the formation and assimilation of microbial community at the anode surface is a time-consuming process. This study utilized a novel unassimilated Ni-foam anode for the first time in solar-powered MEC for bioenergy production. Synthetic dairy manure wastewater (SDMW) was used both as substrate and an inoculum in the solarpowered tubular MEC. The impacts of the exposed surface area of the bio-anode on bioenergy production were evaluated by utilizing two different separation techniques (rate-limited bio-anode -MEC and fully exposed bio-anode -MEC). The former technique achieves a maximum methane production rate of 30.35 ± 0.03 mL/L, 14.2% more than that achieved by the later mentioned technique (26.4 ± 0.05 mL/L). Hydrogen production was approximately 800 ± 5 mm 3 in both experimentations. The maximum generated current in the rate limited bioanode -MEC was 35.5 mA. Scanning electron microscope images confirmed the formation of rod-shaped along with round-shaped microbial communities on the anode surface, and, interestingly, round-shaped bacteria were also grown on the cathode surface. The bioenergy (H 2 and CH 4 ) produced using SDMW within first 13 days of operation, along with the formation of a microbial community, was a significant success in this area and has opened up many research opportunities for producing instant bioenergy from organic waste.

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“…A few studies on AHL-regulated gene clusters identified new bacterial metabolites by addition of exogenous AHLs or genetic engineering to increase transcription of these clusters. [12][13][14] Therefore, exogenous AHLs, N-(3-oxododecanoyl)-L-homoserine lactone and synthesized N-butyryl-L-homoserine lactone (Figure S1) were added to P. aeruginosa cultures at the time of inoculation.…”

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confidence: 99%

Discovery and Biosynthesis of Azabicyclene, a Conserved Nonribosomal Peptide in Pseudomonas aeruginosa

2019

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Azabicyclene, an azetidine-containing natural product, was identified using quorum-sensing molecules to upregulate expression of a gene cluster highly conserved in the human pathogen Pseudomonas aeruginosa. Mutational studies of the gene cluster revealed essential genes for biosynthesis, including an unusual nonribosomal peptide synthetase. Reconstitution of this enzyme in vitro identified key biosynthetic intermediates. This work demonstrates a useful strategy for discovering quorum-sensing-regulated natural products. It sets the stage for understanding the biosynthesis and bioactivity of azabicyclene.

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Sustainable Development Goals

Cited by 13 publications

References 1 publication

The Chemistry and Biology of Bactobolin: A 10-Year Collaboration with Natural Product Chemist Extraordinaire Jon Clardy

The Chemistry and Biology of Bactobolin: A 10-Year Collaboration with Natural Product Chemist Extraordinaire Jon Clardy

Evaluating the use of unassimilated bio‐anode with different exposed surface areas for bioenergy production using solar‐powered microbial electrolysis cell

Discovery and Biosynthesis of Azabicyclene, a Conserved Nonribosomal Peptide in Pseudomonas aeruginosa

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