Editorial on the Research Topic Threatened aquatic gems: freshwater springs and groundwaterdependent ecosystems Groundwater constitutes the Earth's largest liquid freshwater reserve (Stephens et al., 2020). Although awareness is growing that human water demands threaten finite groundwater resources worldwide (Bierkens and Wada, 2019), there remains a dearth of information on how those water demands impact terrestrial and aquatic groundwaterdependent ecosystems (GDEs), including springs. Groundwater withdrawals have reduced inputs to GDEs even in high rainfall areas (Work, 2020), but the threat of groundwater extraction on GDE persistence and condition is especially acute in arid zones where globally, groundwater extraction already exceeds recharge (Aeschback-Hertig and Gleeson, 2012). Worldwide, springs often contain high endemism and can be biodiversity hotspots that contribute to regional biodiversity disproportionate to their size, so ecological consequences of groundwater extraction are potentially severe (Davis et al., 2017).The Research Topic "Threatened Aquatic Gems: Freshwater Springs and Groundwaterdependent Ecosystems" advances our knowledge of the critical role that GDEs play in maintaining biodiversity, and highlights research and conservation strategies needed to protect GDEs, especially in a warming climate.Arid-zone springs have been called "precious jewels of the desert", a reflection of their vulnerability and ecologic importance (Box et al., 2008). The arid spring ecosystems investigated by Fensham et al. in Australia, the southwestern US and Mexico exemplify this vulnerability, as they harbor some of the highest documented concentrations of endemic species in the world. The high endemism of these systems highlights the importance of stability and permanence over evolutionary timescales (up to 47 MYA, Murphy et al., 2015) in producing and maintaining biodiversity. To preserve these biodiversity hotspots will require recognition of their importance and the specific land and water uses that affect their persistence.Globally, climate change will significantly stress GDE health, in part due to groundwater recharge reductions in regions with annual rainfall declines (Wu et al., 2020;Schenk and Stevens, 2022). An investigation of Copepoda from karst springs in Italy by Cerasoli et al.