Sustainable Production of Thermostable Laccase from Agro-Residues Waste by Bacillus aquimaris AKRC02

Kumar, Adarsh; Singh, Ajay; Bilal, Muhammad; Chandra, Ram

doi:10.1007/s10562-021-03753-y

Cited by 25 publications

(8 citation statements)

References 64 publications

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“…Bran and straw can induce and increase the natural mediators and enhanced the rate of laccase-catalyzed oxidation to improve biodegradability [107]. Bran from wheat/rise is a suitable substrate for industrial Lac production that induced Lac activities is reported much higher than various agro-industrial waste residues substrates [108][109][110]. In the present study, straw from corn and rise can improve laccase-producing of SP02.…”

Section: Lignocellulolytic Ssf Charactersmentioning

confidence: 71%

Genomic Studies of White-Rot Fungus Cerrena unicolor SP02 Provide Insights into Food Safety Value-Added Utilization of Non-Food Lignocellulosic Biomass

Zhang

Shah

Mohamed

et al. 2021

JoF

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Cerrena unicolor is an ecologically and biotechnologically important wood-degrading basidiomycete with high lignocellulose degrading ability. Biological and genetic investigations are limited in the Cerrena genus and, thus, hinder genetic modification and commercial use. The aim of the present study was to provide a global understanding through genomic and experimental research about lignocellulosic biomass utilization by Cerrena unicolor. In this study, we reported the genome sequence of C. unicolor SP02 by using the Illumina and PacBio 20 platforms to obtain trustworthy assembly and annotation. This is the combinational 2nd and 3rd genome sequencing and assembly of C. unicolor species. The generated genome was 42.79 Mb in size with an N50 contig size of 2.48 Mb, a G + C content of 47.43%, and encoding of 12,277 predicted genes. The genes encoding various lignocellulolytic enzymes including laccase, lignin peroxidase, manganese peroxidase, cytochromes P450, cellulase, xylanase, α-amylase, and pectinase involved in the degradation of lignin, cellulose, xylan, starch, pectin, and chitin that showed the C. unicolor SP02 potentially have a wide range of applications in lignocellulosic biomass conversion. Genome-scale metabolic analysis opened up a valuable resource for a better understanding of carbohydrate-active enzymes (CAZymes) and oxidoreductases that provide insights into the genetic basis and molecular mechanisms for lignocellulosic degradation. The C. unicolor SP02 model can be used for the development of efficient microbial cell factories in lignocellulosic industries. The understanding of the genetic material of C. unicolor SP02 coding for the lignocellulolytic enzymes will significantly benefit us in genetic manipulation, site-directed mutagenesis, and industrial biotechnology.

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Section: Lignocellulolytic Ssf Charactersmentioning

confidence: 71%

Genomic Studies of White-Rot Fungus Cerrena unicolor SP02 Provide Insights into Food Safety Value-Added Utilization of Non-Food Lignocellulosic Biomass

Zhang

Shah

Mohamed

et al. 2021

JoF

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“…The literature study shows that wheat bran, wheat straw, rice bran, sawdust, corn, apple pomace, canola roots, sugarcane bagasse, cotton stalk,s etc., were identified as potential agro-residue for laccase production through fungi (Ghosh and Ghosh 2017 ; Wang et al 2019 ). There is a maximum production of laccase by Bacillus aquimaris AKRCo2 (4.58 U/mL), when rice bran was used as a substrate for fermentation (Kumar et al 2022 ). A comparison of the laccase production of S. maltophilia E1 and other reported bacterial strains using a cheap source of substrate is presented in Table 6 .…”

Section: Discussionmentioning

confidence: 99%

“…These methods allow researchers to quickly assess the enzymatic capabilities of a large number of bacterial strains, facilitating the discovery of promising candidates (Rodríguez-Escribano et al 2017 ; Sarnaik et al 2020 ). Furthermore, several studies reported the production of laccase by bacteria using low-cost agricultural wastes such as sawdust, corn, apple pomace, canola roots, sugarcane bagasse, cotton stalks and wheat bran (Kumar et al 2022 ). These agriculture residues pose a serious threat to the environment if not managed properly.…”

Section: Introductionmentioning

confidence: 99%

Optimization of laccase from Stenotrophomonas maltophilia E1 by submerge fermentation using coconut husk with its detoxification and biodecolorization ability of synthetic dyes

Albulaihed,

Adnan,

Jamal

et al. 2023

Bioresour. Bioprocess.

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Enzymatic degradation of synthetic dyes holds an immense promise for addressing the environmental concerns associated with the textile and dye industries. This study aimed to isolate bacteria capable of producing laccase enzymes from an anthropogenic environment. Subsequently, viability of utilizing cost-effective agricultural residues as substrates for laccase production was assessed. Response Surface Methodology (RSM) and the One Variable at a Time (OVAT) approach was pursued for the optimization of laccase production, followed by pH and temperature stability, dye degradation and decolorization experiments, toxicological studies on the degraded dye metabolites. In results, laccase-producing bacterial strain was identified as Stenotrophomonas maltophilia strain E1 (S. maltophilia). Among variety of substrates, coconut husk exhibited optimal efficacy. In a statistical optimization study, it was found that S. maltophilia was capable of producing laccase 51.38 IU/mL, i.e., three times higher than the amount of laccase produced by unoptimized medium (16.7 IU/mL), and the enzyme activity was found to be steady at an acidic pH, and a mesophilic temperature range. The laccase obtained from S. maltophilia E1 demonstrated proficient dye decolorization capabilities, achieving a notable 92.1% reduction in Malachite green dye coloration at a concentration of 500 ppm. Gas chromatography–mass spectrometry (GC–MS) analysis of the decolorized derivatives of Malachite green revealed a conversion into a distinct compounds. Moreover, after undergoing laccase treatment, Malachite green exhibited decreased phytotoxic effects on Oryza sativa, pointing to enzymatic detoxification. Collectively, insights gained from the present study will contribute to the development of efficient enzymatic approaches for addressing the environmental pollution caused by synthetic dyes. Graphical Abstract

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“…Lignocellulosic biomass, considered as the most abundant and renewable resource on earth, is mainly composed of cellulose (30 to 50% of dry matter weight), hemicellulose (20 to 40% of dry matter weight), and lignin (15 to 25% of dry matter weight), in addition to a small amount of structural proteins, lipids, and ash. Certainly, a part of it is usually considered as wastes, like corncob, leaves, sweet sorghum bagasse, coffee shell, corn stover, wheat straw, and sugar cane (Zhou et al 2014;Sun et al 2015;Moreira et al 2016;Huang et al 2019;Karagoz et al 2019;Kumar et al 2021). Generally, the main way of disposing of lignocellulosic wastes is to discard it to rot or burn it directly.…”

Section: Introductionmentioning

confidence: 99%

Extracellular laccase activity among Ganoderma and Coriolopsis species grown on lignocellulosic wastes

Han

Yang

Jian-jun

et al. 2022

BioRes

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The extensive application of laccase in various fields depends on the supply of a large amount of laccase with high activity and low cost. Interest on the screening of fungal strains suitable for obtaining massive high-activity laccase in solid-state fermentation with suitable low-cost lignocellulosic wastes has increased. The present work determined the laccase secretion from different species belonging to genera Ganoderma and Coriolopsis fermented on different lignocellulosic wastes. Maximal laccase activity was obtained from Ganoderma lingzhi Han 1345 grown on rice straw for 9 days and indicated the capacity of secreting laccase of G. lingzhi Han 1345 was superior to that of Coriolopsis trogii Han 1211, C. strumosa Han 1356, and G. applanatum Han 1578. The presence of cottonseed hull was more favorable for Coriolopsis species secreting laccase, while Ganoderma species were more preferred to secrete laccase on rice straw. Further, the consistent substrate preference in laccase production of different species in the same genus was first exhibited. The results are useful to screen and obtain new species with superior capacity of secreting laccase and suitable low-cost lignocellulosic wastes as fermentation substrate for further industrial application of laccase.

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Sustainable Production of Thermostable Laccase from Agro-Residues Waste by Bacillus aquimaris AKRC02

Cited by 25 publications

References 64 publications

Genomic Studies of White-Rot Fungus Cerrena unicolor SP02 Provide Insights into Food Safety Value-Added Utilization of Non-Food Lignocellulosic Biomass

Genomic Studies of White-Rot Fungus Cerrena unicolor SP02 Provide Insights into Food Safety Value-Added Utilization of Non-Food Lignocellulosic Biomass

Optimization of laccase from Stenotrophomonas maltophilia E1 by submerge fermentation using coconut husk with its detoxification and biodecolorization ability of synthetic dyes

Extracellular laccase activity among Ganoderma and Coriolopsis species grown on lignocellulosic wastes

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