Sustainable repairing and improvement of concrete properties using artificial bacterial consortium

Mahmoud, Hala H. A.; Kalaba, Mohamed H.; El-Sherbiny, Gamal M.; Mostafa, A.; Ouf, Mohamed; Tawhed, Waleed M. F.

doi:10.1080/21650373.2021.2003908

Cited by 6 publications

(3 citation statements)

References 54 publications

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“…CaCO 3 precipitates formed by each isolate were independently subjected to acid fizz test by overlaying the precipitate with 10% (v/v) HCl in each tube (Mahmoud et al, 2021;Abudoleh et al, 2018). Generation of effervescence after adding HCl is to be treated as a positive test.…”

Section: Acid Fizz Testmentioning

confidence: 99%

Calcium carbonate precipitation by Paenibacillus fonticola and Sporosarcina luteola for its potential application in bio concrete

Kakad,

Shede

2023

EEC

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Concrete is one of the most widely used construction materials in various infrastructures. However, despite of several advantages, it has a high tendency to form cracks due to climatic effects and parameters like temperature fluctuations, exposure to corrosive and toxic chemicals, harmful gases, natural disasters, etc. These factors reduce the longevity of concrete which adds up to the cost of the maintenance and repair. Existing treatments have various limitations like weak resistance to weather, moisture sensitivity, heat and low sustainability. Hence, there is a need for alternative and sustainable treatment technologies. One such technology is bio concrete formation by the process of Microbially Induced Calcium Carbonate Precipitation (MICP). In nature, MICP occurs through different processes like urea hydrolysis, photosynthesis, denitrification, etc. Bio concrete is a combination of concrete and bacteria possessing the ability to precipitate calcium carbonate (CaCO3 ) that aids in sealing the cracks that appear in it. This self-healing property of bio concrete can be a potentially sustainable and economic technology. This study was aimed to enrich, isolate and screen bacteria capable of precipitating CaCO3 and their use in production of bio concrete. Total 11 samples predominantly containing speleothems and related materials were collected from different locations in Satara and Nashik districts of Maharashtra, India. The samples were further processed independently in B4 and Calcium Carbonate Precipitation (CCP) media for enrichment. A total of 94 isolates were obtained from the enriched samples. Subsequent screening and characterization of these isolates revealed that Paenibacillus fonticola and Sporosarcina luteola can substantially precipitate calcium carbonate.The Fourier Transform Infrared (FTIR) spectra, Scanning Electron Microscopy (SEM) and Energy Dispersive X-ray (EDX) microanalysis of the CaCO3 precipitates formed by these bacteria renders both Paenibacillus fonticola and Sporosarcina luteola the potential candidature for bioconcrete formation.

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Section: Acid Fizz Testmentioning

confidence: 99%

Calcium carbonate precipitation by Paenibacillus fonticola and Sporosarcina luteola for its potential application in bio concrete

Kakad,

Shede

2023

EEC

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“…Further, generally, the consortium microbes show more resistance to the environmental effects than pure cultures. [30][31][32] Consortium bacteria often contain many collaborating bacterial populations, which have the ability to exchange metabolism activity and convey molecular signals. According to previous research outcomes, a consortium of mixed cultures can produce minerals more effectively and economically than that of pure culture.…”

Section: Introductionmentioning

confidence: 99%

“…The adverse conditions could potentially hinder the MICP process in pure bacterial cultures due to expected competition among different bacterial species. Further, generally, the consortium microbes show more resistance to the environmental effects than pure cultures 30–32 . Consortium bacteria often contain many collaborating bacterial populations, which have the ability to exchange metabolism activity and convey molecular signals.…”

Section: Introductionmentioning

confidence: 99%

Effects of bacterial consortium enhanced recycled coarse aggregates on self‐healing concrete immobilized with Bacillus megaterium MTCC 1684 and Bacillus subtilis NCIM 2193

Bakr,

Hussain,

Singh

et al. 2023

Structural Concrete

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The strength and durability properties of the recycled aggregate concrete (RAC) have been affected by the cracks and the weak interfacial transition zone (ITZ) of the recycled coarse aggregates (RCA). However, the mechanical and physical features of RCA can be improved by microbially induced calcite precipitation (MICP). Therefore, immobilization techniques were used to protect and maintain the high efficiency of Bacillus bacteria for the formation and precipitation of calcium carbonates in self‐healing concrete over a period of time. The objective of the present study was to show the viability of the immobilized bacterial consortium‐enhanced RCA to form self‐healing cracks. Further, the self‐healing capability of enhanced RCA was investigated along with two other immobilization methods, that is, RCA and hydrated lime and brick powder (HBr)‐immobilized bacteria. The experimental results show that the increase in the bio‐deposition time improved the physical and mechanical properties of the RCA. Further, subsequently 56 days of the healing incubation period, the immobilized consortium‐enhanced RCA concrete specimens completely healed the cracks of width 0.58 mm. However, the equivalent cracks of width 0.56 mm were also recovered by the HBr immobilized bacterial cultures. Furthermore, the field emission scanning electron microscope (FESEM), energy dispersive spectroscopy (EDS) and X‐ray diffraction (XRD) analysis revealed that the existence of precipitation at the crack surface was calcium carbonate with a regular cubic‐shaped and lamellar layer morphology. The outcomes of the current study show that consortium‐enhanced RCA has promising potential to develop self‐healing concrete with self‐repaired and improved durability properties in the concrete construction field.

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Investigation on the enhancement of crack restoration properties in cement incorporated with Arthrospira platensis cultured in modified medium

Alengaram,

Ibrahim

et al. 2024

Environ Sci Pollut Res

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Sustainable repairing and improvement of concrete properties using artificial bacterial consortium

Cited by 6 publications

References 54 publications

Calcium carbonate precipitation by Paenibacillus fonticola and Sporosarcina luteola for its potential application in bio concrete

Calcium carbonate precipitation by Paenibacillus fonticola and Sporosarcina luteola for its potential application in bio concrete

Effects of bacterial consortium enhanced recycled coarse aggregates on self‐healing concrete immobilized with Bacillus megaterium MTCC 1684 and Bacillus subtilis NCIM 2193

Investigation on the enhancement of crack restoration properties in cement incorporated with Arthrospira platensis cultured in modified medium

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