Sustained bacterial N₂O reduction at acidic pH

Abstract

Nitrous oxide (N2O) is a climate-active gas and emissions from terrestrial ecosystems are concerning. Microbial reduction of N2O to dinitrogen (N2) is the only known consumption process and has been studied extensively at circumneutral pH; however, N2O reduction under acidic conditions is thought to be limited. Global soil acidification, accelerated by anthropogenic practices, introduces high uncertainty into N2O emission budgets. We obtained an enrichment culture from an acidic tropical forest soil that robustly reduces N2O to N2at pH 4.5 with the addition of pyruvate and hydrogen. Consecutive transfers at pH 4.5 yielded a co-culture and temporal analyses revealed a bimodal growth pattern with aSerratiasp. growing during the initial pyruvate fermentation phase followed by growth of a novelDesulfosporosinussp. via hydrogenotrophic N2O reduction. TheDesulfosporosinussp. produced (3.1 ± 0.11) × 108cells per mmol of N2O consumed, on par with growth yields reported for clade II N2O reducers at circumneutral pH. Genome analysis identified a clade IInosgene cluster, but an incomplete pathway for sulfate reduction, a hallmark feature of the genusDesulfosporosinus. Physiological and metabogenomic characterization revealed interspecies nutritional interactions, with the pyruvate fermentingSerratiasp. supplying amino acids as essential growth factors to theDesulfosporosinussp. The co-culture reduced N2O between pH 4.5 and 6 but not at or above pH 7, contradicting the paradigm that sustained microbial N2O reduction ceases under acidic pH conditions, thus confirming a previously unrecognized N2O reduction potential in acidic soils.Significance StatementProcesses generating N2O occur over a broad pH range spanning pH 3 to 12; however, the current paradigm assumes that microbial N2O consumption is limited to circumneutral pH (6 to 8). The imbalance between N2O production versus consumption has increased the atmospheric concentration of this climate active gas by 17 % over the last 100 years, and accelerated emissions due to global soil acidification are a major climate concern. From acidic soil, we obtained a bacterial culture harboring a novelDesulfosporosinusspecies that effectively reduces N2O at pH 4.5, but not at or above pH 7. The discovery of an N2O reducer adapted to acidic pH conditions has far-reaching implications for predicting, modeling, and potentially managing N2O emissions from low pH ecosystems.Note for publisher (this text will be removed prior to publication)This manuscript has been authored by UT-Battelle, LLC under Contract No. DE-AC05-00OR22725 with the U.S. Department of Energy. The United States Government retains and the publisher, by accepting the article for publication, acknowledges that the United States Government retains a non-exclusive, paid-up, irrevocable, world-wide license to publish or reproduce the published form of this manuscript, or allow others to do so, for United States Government purposes. The Department of Energy will provide public access to these results of federally sponsored research in accordance with the DOE Public Access Plan (http://energy.gov/downloads/doe-public-access-plan).