2014
DOI: 10.1016/j.jim.2014.02.005
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Sustained in vivo depletion of splenic langerin+ CD8α+ dendritic cells is well-tolerated by lang-DTREGFP mice

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Cited by 9 publications
(17 citation statements)
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“…However, when DT was administered after 4 h, the response was not affected. We showed previously that langerin + CD8a + DCs are depleted in the spleen within 4 h of DT administration (18). Taking this into account, we conclude that langerin + CD8a + DCs were required for only 6-8 h after Ag administration.…”
Section: Resultssupporting
confidence: 58%
See 1 more Smart Citation
“…However, when DT was administered after 4 h, the response was not affected. We showed previously that langerin + CD8a + DCs are depleted in the spleen within 4 h of DT administration (18). Taking this into account, we conclude that langerin + CD8a + DCs were required for only 6-8 h after Ag administration.…”
Section: Resultssupporting
confidence: 58%
“…treatment of 350 ng DT (Sigma-Aldrich, Auckland, New Zealand) 1 d prior to Ag and stimuli administration, resulting in high depletion efficiency, as previously described (18), SiglecH-DTR mice were depleted of plasmacytoid DCs (pDCs) with DT at days 1 and 2 prior to Ag administration (Supplemental Fig. 1).…”
Section: Depletion In Vivomentioning
confidence: 99%
“…To determine if splenic langerin + CD8α + DCs were required for control of systemic BCG infection, we used lang- DTREGFP mice (referred to as Lang-DTR mice), which allowed depletion of langerin-expressing cells with DT during the course of infection. Multiple doses of DT were well tolerated and resulted in effective depletion of langerin + CD8α + DCs in the spleen [Figures 1 A,B; ( 26 )]. Depletion of langerin − CD8α + DCs was not evident (Figure S1 in Supplementary Material).…”
Section: Resultsmentioning
confidence: 99%
“…In the indicated experiments, 350 ng DT (Sigma-Aldrich, St Louis, MO) was administered every 2 days intraperitoneally to lang-EGFPDTR mice, resulting in a sustained depletion (> 90%) of langerin-expressing cells in the spleen (Supplementary Figure 1, Supplemental Digital Content 1, http:// links.lww.com/JIT/A397). 13,16 Adoptive Transfer of Naive T Cells Single-cell suspensions were prepared from lymph nodes from OT-I ÂB6-Sj ptprc a mice and labeled with 1.25 mM 5-(and 6)-carboxyfluorescein diacetate succinimidyl ester (CFSE). Mice were administered 5Â 10 6 cells through the lateral tail vain.…”
Section: Challenge With Lymphoma Cellsmentioning
confidence: 99%