Sustained increases in plasma C-type natriuretic peptides fail to increase concentrations in cerebrospinal fluid: Evidence from pregnant sheep

Wilson, Michele O.; Barrell, Graham K.; Prickett, Timothy C. R.; Espiner, Eric A.

doi:10.1016/j.peptides.2015.04.016

Cited by 7 publications

(2 citation statements)

References 30 publications

(65 reference statements)

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“…, 1995 ) and N‐terminal proCNP (NTproCNP) (Wilson et al. , 2015 ), were measured by radioimmunoassay (RIA) as previously described. Non‐extracted plasma renin activity (PRA) (Dunn & Espiner, 1976 ) and aldosterone (Lun et al.…”

Section: Methodsmentioning

confidence: 99%

“…Venous blood samples were obtained at 30 min and immediately preceding commencement of the first infusion (baseline) and at 30 min intervals for the duration of the study day. Samples were taken into tubes on ice, centrifuged at 4 et al, 1990), ANP (Charles et al, 1990), BNP (Pemberton et al, 1997), CNP (Charles et al, 1995) and N-terminal proCNP (NTproCNP) (Wilson et al, 2015), were measured by radioimmunoassay (RIA) as previously described. Non-extracted plasma renin activity (PRA) (Dunn & Espiner, 1976) and aldosterone (Lun et al, 1983) (Hirata et al, 1990).…”

Section: Study Protocolmentioning

confidence: 99%

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Haemodynamic, hormonal and renal actions of osteocrin in normal sheep

Scott,

Prickett,

Charles

et al. 2024

Experimental Physiology

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Osteocrin (OSTN) is an endogenous protein sharing structural similarities with the natriuretic peptides [NPs; atrial (ANP), B‐type (BNP) and C‐type (CNP) NP], which are hormones known for their crucial role in maintaining pressure/volume homeostasis. Osteocrin competes with the NPs for binding to the receptor involved in their clearance (NPR‐C). In the present study, having identified, for the first time, the major circulating form of OSTN in human and ovine plasma, we examined the integrated haemodynamic, endocrine and renal effects of vehicle‐controlled incremental infusions of ovine proOSTN (83–133) and its metabolism in eight conscious normal sheep. Incremental i.v. doses of OSTN produced stepwise increases in circulating concentrations of the peptide, and its metabolic clearance rate was inversely proportional to the dose. Osteocrin increased plasma levels of ANP, BNP and CNP in a dose‐dependent manner, together with concentrations of their intracellular second messenger, cGMP. Increases in plasma cGMP were associated with progressive reductions in arterial pressure and central venous pressure. Plasma cAMP, renin and aldosterone were unchanged. Despite significant increases in urinary cGMP levels, OSTN administration was not associated with natriuresis or diuresis in normal sheep. These results support OSTN as an endogenous ligand for NPR‐C in regulating plasma concentrations of NPs and associated cGMP‐mediated bioactivity. Collectively, our findings support a role for OSTN in maintaining cardiovascular homeostasis.

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Section: Methodsmentioning

confidence: 99%

Section: Study Protocolmentioning

confidence: 99%

Haemodynamic, hormonal and renal actions of osteocrin in normal sheep

Scott,

Prickett,

Charles

et al. 2024

Experimental Physiology

Self Cite

View full text Add to dashboard Cite

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Natriuretic Peptides and Normal Body Fluid Regulation

Bie

2018

Comprehensive Physiology

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Natriuretic peptides are structurally related, functionally diverse hormones. Circulating atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) are delivered predominantly by the heart. Two C-type natriuretic peptides (CNPs) are paracrine messengers, notably in bone, brain, and vessels. Natriuretic peptides act by binding to the extracellular domains of three receptors, NPR-A, NPR-B, and NPR-C of which the first two are guanylate cyclases. NPR-C is coupled to inhibitory proteins. Atrial wall stress is the major regulator of ANP secretion; however, atrial pressure changes plasma ANP only modestly and transiently, and the relation between plasma ANP and atrial wall tension (or extracellular volume or sodium intake) is weak. Absence and overexpression of ANP-related genes are associated with modest blood pressure changes. ANP augments vascular permeability and reduces vascular contractility, renin and aldosterone secretion, sympathetic nerve activity, and renal tubular sodium transport. Within the physiological range of plasma ANP, the responses to step-up changes are unimpressive; in man, the systemic physiological effects include diminution of renin secretion, aldosterone secretion, and cardiac preload. For BNP, the available evidence does not show that cardiac release to the blood is related to sodium homeostasis or body fluid control. CNPs are not circulating hormones, but primarily paracrine messengers important to ossification, nervous system development, and endothelial function. Normally, natriuretic peptides are not powerful natriuretic/diuretic hormones; common conclusions are not consistently supported by hard data. ANP may provide fine-tuning of reno-cardiovascular relationships, but seems, together with BNP, primarily involved in the regulation of cardiac performance and remodeling. © 2017 American Physiological Society. Compr Physiol 8:1211-1249, 2018.

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Physically open BBB

Chen

Zhao

2019

Brain Targeted Drug Delivery System

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Sustained increases in plasma C-type natriuretic peptides fail to increase concentrations in cerebrospinal fluid: Evidence from pregnant sheep

Cited by 7 publications

References 30 publications

Haemodynamic, hormonal and renal actions of osteocrin in normal sheep

Haemodynamic, hormonal and renal actions of osteocrin in normal sheep

Natriuretic Peptides and Normal Body Fluid Regulation

Physically open BBB

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