Sustained release of growth factors from photoactivated platelet rich plasma (PRP)

Irmak, Gülseren; Demirtaş, Tuǧrul Tolga; Gümüşderelı́oğlu, Menemşe

doi:10.1016/j.ejpb.2019.11.011

Cited by 44 publications

(38 citation statements)

References 27 publications

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“…Light exposed PLTs were characterized by extended lamellipodia, numerous filopodia, and PLT agglomeration. P-selectin expression was significantly increased 31 .…”

Section: Discussionmentioning

confidence: 92%

Near-infrared photobiomodulation of blood reversibly inhibits platelet reactivity and reduces hemolysis

Walski

Grzeszczuk-Kuć

Gałecka

et al. 2022

Sci Rep

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Photobiomodulation (PBM) in the red/near-infrared (R/NIR) spectral range has become widely recognized due to its anti-inflammatory and cytoprotective potential. We aimed to assess the effects of blood PBM on platelets function and hemolysis in an in vitro setting. Porcine blood samples were separated into four aliquots for this study, one of which served as a control, while the other three were subjected to three different NIR PBM dosages. The platelet count and functions and the plasma free haemoglobin and osmotic fragility of red blood cells were measured during the experiment. The control group had a considerable drop in platelet number, but the NIR exposed samples had more minimal and strictly dose-dependent alterations. These modifications were consistent with ADP and collagen-induced platelet aggregation. Furthermore, red blood cells that had received PBM were more resistant to osmotic stress and less prone to hemolysis, as seen by a slightly lower quantity of plasma free hemoglobin. Here we showed under well-controlled in vitro conditions that PBM reversibly inhibits platelet activation in a dose-dependent manner and reduces hemolysis.

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“…Light exposed PLTs were characterized by extended lamellipodia, numerous filopodia, and PLT agglomeration. P-selectin expression was significantly increased 31 .…”

Section: Discussionmentioning

confidence: 92%

Near-infrared photobiomodulation of blood reversibly inhibits platelet reactivity and reduces hemolysis

Walski

Grzeszczuk-Kuć

Gałecka

et al. 2022

Sci Rep

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“…In our study, we activated our PRP with calcium activator and laser before intravenous administration. Photoactivation of platelets by a low-frequency light source results in controlled and prolonged release of growth factors (15). The usage of photoactivated PRP has been reported in studies with positive results (7,10,16).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of plasma PDGF and VEGF levels after systemic administration of activated autologous platelet-rich plasma

Karina¹,

Ekaputri²,

Biben³

et al. 2021

Biomedicine

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Introduction and Aim: Activated autologous platelet-rich plasma (aaPRP) is becoming a popular therapy to accelerate healing in the field of plastic surgery. Platelets, which are abundant in aaPRP, can release many growth factors including platelet-derived growth factor (PDGF) and vascular endothelial growth factor (VEGF). This study aims to examine the plasma levels of PDGF and VEGF in healthy subjects after intravenous administration of aaPRP. Materials and Methods: Nine healthy patients with no prior history of metabolic disease were divided into two groups (control and experiment group). The treatment group which consists of six patients received intravenous aaPRP treatment. The preparation of aaPRP starts with the collection of 24 mL of whole blood in sodium citrate tubes followed a two-step centrifugation procedure and subsequent chemical activation. aaPRP was then administered intravenously to patients. Meanwhile, the control group received no intervention. Venous blood samples were taken before and one week after the treatment and the plasma PDGF and VEGF levels were determined by enzyme-linked immunosorbent assay (ELISA). Results: The treatment group showed statistically significant increase in VEGF after 7 days compared to before aaPRP administration. However, the change in PDGF levels of the treatment group was unnotable. Conclusion: The present findings indicate that intravenous administrations of activated aaPRP may increase plasma VEGF level up to 1 week after aaPRP administration.

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“…Photo-activation has been suggested as an alternative method to trigger platelet activation: a very recent paper [48] described in vitro characterization of platelet photo-activation (polychromatic light source, in the range near-infrared region), in comparison with resting platelets and calcium chloride mediated PRP activation. That study showed that photo-activation of PRP induced a significantly more prolonged release and higher amount of platelet-derived growth factor (PDGF), basic fibroblast growth factor (FGF), and transforming growth factor (TGF)-beta than PRP activated with calcium chloride.…”

Section: Activation Processmentioning

confidence: 99%

Platelet Concentrates in Musculoskeletal Medicine

Mariani

Pulsatelli

2020

IJMS

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Platelet concentrates (PCs), mostly represented by platelet-rich plasma (PRP) and platelet-rich fibrin (PRF) are autologous biological blood-derived products that may combine plasma/platelet-derived bioactive components, together with fibrin-forming protein able to create a natural three-dimensional scaffold. These types of products are safely used in clinical applications due to the autologous-derived source and the minimally invasive application procedure. In this narrative review, we focus on three main topics concerning the use of platelet concentrate for treating musculoskeletal conditions: (a) the different procedures to prepare PCs, (b) the composition of PCs that is related to the type of methodological procedure adopted and (c) the clinical application in musculoskeletal medicine, efficacy and main limits of the different studies.

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Sustained release of growth factors from photoactivated platelet rich plasma (PRP)

Cited by 44 publications

References 27 publications

Near-infrared photobiomodulation of blood reversibly inhibits platelet reactivity and reduces hemolysis

Near-infrared photobiomodulation of blood reversibly inhibits platelet reactivity and reduces hemolysis

Evaluation of plasma PDGF and VEGF levels after systemic administration of activated autologous platelet-rich plasma

Platelet Concentrates in Musculoskeletal Medicine

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