Sustained release of lysozyme from zein microcapsules produced by a supercritical anti-solvent process

Zhong, Qixin; Jin, Minfeng; Davidson, P. Michael; Zivanovic, Svetlana

doi:10.1016/j.foodchem.2008.12.063

Cited by 92 publications

(53 citation statements)

References 9 publications

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“…After processing, the peak position moved to a higher angle 22°, meanwhile the intensity decreased. This is consistent with other researchers' conclusion 14,23 that the degree of crystallinity is reduced through the SAS process. The XRD spectra shown in Figure 7 also clearly indicate that the zein protein has an amorphous nature.…”

Section: Resultssupporting

confidence: 93%

“…Zein is a kind of protein made from maize. It has been widely utilized in drug release systems because of its good biocompatibility, eg, 5-fluorouracil-loaded zein nanoparticles 22 and lysozyme-loaded zein microcapsules, 23 etc. It was also used as a carrier in release systems in the field of food engineering.…”

Section: Introductionmentioning

confidence: 99%

“…It was also used as a carrier in release systems in the field of food engineering. 23,24 The purpose of this research is to explore the preparation of nanoparticles by using the SEDS approach combining the CFD simulation. Zein was chosen as a model compound.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Preparation of zein nanoparticles by using solution-enhanced dispersion with supercritical CO<sub>2</sub> and elucidation with computational fluid dynamics

Li¹,

Zhao²

2017

IJN

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Nanoparticles have attracted more and more attention in the medicinal field. Zein is a biomacromolecule and can be used as a carrier for delivering active ingredients to prepare controlled release drugs. In this article, we presented the preparation of zein nanoparticles by solution-enhanced dispersion by supercritical CO 2 (SEDS) approach. Scanning electron microscopy and transmission electron microscopy were applied to characterize the size and morphology of the obtained particles. The nozzle structure and the CO 2 flow rate greatly affected the morphology and the size of the particles. The size of zein was able to be reduced to 50–350 nm according to the different conditions. The morphologies of the resultant zein were either sphere or the filament network consisted of nanoparticles. The influence of the nozzle structure and the CO 2 flow rate on the velocity field was elucidated by using computational fluid dynamics. The nozzle structure and the CO 2 flow rate greatly affected the distribution of the velocity field. However, a similar velocity field could also be obtained when the nozzle structure or the CO 2 flow rate, or both were different. Therefore, the influence of the nozzle structure and the CO 2 flow rate on the size and morphology of the particles, can boil down to the velocity field. The results demonstrated that the velocity field can be a potential criterion for producing nanoparticles with controllable morphology and size, which is useful to scale-up the SEDS process.

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Section: Resultssupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Preparation of zein nanoparticles by using solution-enhanced dispersion with supercritical CO<sub>2</sub> and elucidation with computational fluid dynamics

Li¹,

Zhao²

2017

IJN

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“…Zein films and zein micro/nano particles can be used for encapsulation of essential oils, aromas and flavors, controlled release of active additives and as an active food packaging material, etc. (Alkan et al, 2011;Parris, Cooke, & Hicks, 2005;Patel, Heussen, Hazekamp, Drost, & Velikov, 2012;Sanchez-Garcia, Hilliou, & Lagaron, 2010;Shi, Kokini, & Huang, 2009;Zhong, Jin, Davidson, & Zivanovic, 2009). In the recent years, electrospinning of zein nanofibers have received much attention as well (Jiang, Reddy, & Yang, 2010;Jiang & Yang, 2011;Jiang, Zhao, & Zhu, 2007;Miyoshi, Toyohara, & Minematsu, 2005;Selling et al, 2007Torres-Giner, Gimenez, & Lagaron, 2008;).…”

Section: Introductionmentioning

confidence: 99%

Electrospun zein nanofibers incorporating cyclodextrins

Kayacı

Uyar

2012

Carbohydrate Polymers

129

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a b s t r a c tZein nanofibers containing cyclodextrins (zein/CD) were produced via electrospinning. Three types of CDs (␣-CD, ␤-CD and ␥-CD) having 10%, 25% and 50% (w/w) were individually incorporated into zein nanofibers. SEM imaging elucidated that the morphologies of the electrospun zein/CD nanofibers depended on the CD type and weight percentage. The incorporation of CDs in zein improved the electrospinnability and bead-free nanofibers were obtained at lower zein concentrations. Zein/CD nanofibers having fiber diameters ∼100-400 nm were obtained depending on the zein concentrations, types and weight percentages of CD. XRD studies revealed that CDs were mostly distributed without forming crystalline aggregates for zein/CD nanofibers containing lower weight percentage of CDs. The surface analyses of zein/CD nanofibers by ATR-FTIR and XPS indicated that some of the CDs were present on the fiber surface. Thermal analyses showed that zein/␤-CD nanofibers have shown higher glass transition temperatures and higher degradation temperature with increasing CD content.

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“…Antioxidants such as a-tocopherol, quercetin, Vitamin E, Vitamin B12, tea phenol and antimicrobial agents, trans-2-hexenal, cinnamon essential oil, nisin and thymol were incorporated into hydrophilic/hydrophobic films in encapsulated form (Bao, Xu, & Wang, 2009;Imran et al, 2012;Joo, Merkel, Auras, & Almenar, 2012;Koontz et al, 2010;Li, Yin, Yang, Tang, & Wei, 2012;Noronha, de Carvalho, Lino, & Barreto, 2014;Sir o et al, 2006;Wu et al, 2015). Lysozyme was also encapsulated in zein microcapsules (Zhong, Jin, Davidson, & Zivanovic, 2009) and starch microgel ; however, these particles were not utilized for developing food packaging films. Although sustained release of lysozyme from zein capsules was observed, the use of these particles in hydrophilic food packaging materials seems to be highly challenging since homogeneous distribution of hydrophobic zein particles in hydrophilic film structures is a difficult task.…”

Section: Introductionmentioning

confidence: 99%

Development of a novel strategy for controlled release of lysozyme from whey protein isolate based active food packaging films

Ozer

Oymacı

et al. 2016

Food Hydrocolloids

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a b s t r a c tThe purpose of this study is to develop a novel controlled release system based on pH-responsive polyacrylic acid (PAA)/lysozyme (LYS) complexes incorporated within a hydrophilic whey protein isolate (WPI) film matrix for active food packaging applications. Complex formation is simple under benign conditions that are suitable for preserving antimicrobial activity of the lysozyme. In addition, the pHdependent charge density of complexes allowed a uniform distribution in the matrix. The properties of the complexes such as size, surface charge and hydrophilicity were varied by changing PAA/LYS ratio (0.1 and 0.3 w/w) and PAA molecular weight (2 kDa and 450 kDa). The effects of complex properties as well as mode of lysozyme incorporation into the films (100%-free, 50%-freeþ50%-PAA/LYS complex and 100%-PAA/LYS complex) on the LYS release rate, activity and antimicrobial efficacy of the films were investigated. The results have shown that~100% LYS loading into the complexes is possible regardless of PAA molecular weight or PAA/LYS ratio. Incorporating lysozyme into the film in complexed form extended its release time from less than 24 h up to 500 h and reduced its diffusivity from~10 À9 tõ 10 À13 cm 2 /s. The films including 50%-free-LYSþ50%-PAA/LYS complex showed a 5.7 log reduction in bacterial population within 72 h whereas 100%-free-LYS containing film could not suppress Listeria innocua growth after 24 h. Overall, the results suggest that complexation of lysozyme with weak polyelectrolytes can be used as an effective strategy to achieve a long-lasting antimicrobial effect and that films prepared with such complexes have great potential as food packaging materials.

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Sustained release of lysozyme from zein microcapsules produced by a supercritical anti-solvent process

Cited by 92 publications

References 9 publications

Preparation of zein nanoparticles by using solution-enhanced dispersion with supercritical CO<sub>2</sub> and elucidation with computational fluid dynamics

Preparation of zein nanoparticles by using solution-enhanced dispersion with supercritical CO<sub>2</sub> and elucidation with computational fluid dynamics

Electrospun zein nanofibers incorporating cyclodextrins

Development of a novel strategy for controlled release of lysozyme from whey protein isolate based active food packaging films

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