Sustained substrate cycles between hexose phosphates and free sugars in phosphate-deficient potato (Solanum tuberosum) cell cultures

He, Jialin; Dorion, Sonia; Lacroix, Mélanie; Rivoal, Jean

doi:10.1007/s00425-019-03088-4

Cited by 3 publications

(25 citation statements)

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“…Chromatographic resins (Dowex AG 50W-X8 (H + ) and Dowex AG 1-X8 (formate)) were obtained from Bio-Rad Laboratories (Mississauga, ON, Canada). Potato heterotrophic cell cultures established in our laboratory [32] were subcultured every 14 days and grown in a Murashige and Skoog (MS) medium [33], exactly as described previously for +Pi (inoculated with 2.5 mM Pi) or −Pi (inoculated with 125 µM Pi) conditions [24]. Cells were grown on a gyratory shaker at 140 rpm at 22 • C. They were harvested by centrifugation (4500× g for 10 min) at different times of the culture cycle and further used for labeling studies.…”

Section: Chemicals and Plant Materialsmentioning

confidence: 99%

“…Our results are consistent with the view that Suc catabolic fluxes also remain relatively stable in cells experiencing Pi deprivation. Induction of PEPC activity in plants subjected to Pi deficiency has been documented in several systems [20][21][22][23][24]. In Pi-deficient potato cell cultures, significant concomitant increases in PEPC activity and PEPC protein have been documented [24].…”

Section: Sugar Catabolic Fluxes Are Similar In +Pi and −Pi Cellsmentioning

confidence: 99%

“…In a previous study, we addressed Pi nutrition in potato cell cultures [24]. We surveyed the activities of a large number of glycolytic and respiratory enzymes and the level of key primary metabolites in Pi-sufficient (+Pi) and Pi-deficient (−Pi) conditions [24]. In the present study, we used the same experimental system to investigate the metabolism of [U- 14 C]sucrose (Suc) and NaH 14 CO 3 in order to test the hypothesis that changes in central metabolic fluxes occur in response to modifications in Pi nutrition.…”

Section: Introductionmentioning

confidence: 99%

“…There is also ample evidence that plants exploit built-in flexibility in their primary metabolism and enhance the activity of enzymes that conserve Pi and ATP during Pi starvation, in particular in the glycolytic pathway [ 19 , 20 ]. For instance, induction of phospho enol pyruvate carboxylase (PEPC) and/or phospho enol pyruvate phosphatase activities under Pi-deficient conditions has been demonstrated in various systems [ 20 , 21 , 22 , 23 , 24 ]. These two enzymes bypass the reaction of ADP-dependent pyruvate kinase and generate, respectively, oxaloacetate and pyruvate needed to supply C to the TCA cycle.…”

Section: Introductionmentioning

confidence: 99%

“…Given the major importance of Pi in metabolism, it is thus essential to expand our understanding of the impact of Pi deficiency on C fluxes in potato. In a previous study, we addressed Pi nutrition in potato cell cultures [ 24 ]. We surveyed the activities of a large number of glycolytic and respiratory enzymes and the level of key primary metabolites in Pi-sufficient (+Pi) and Pi-deficient (−Pi) conditions [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

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Carbon Fluxes in Potato (Solanum tuberosum) Remain Stable in Cell Cultures Exposed to Nutritional Phosphate Deficiency

He,

Dorion,

Carmona-Rojas

et al. 2023

Biology

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Nutritional phosphate deficiency is a major limitation to plant growth. Here, we monitored fluxes in pathways supporting respiratory metabolism in potato (Solanum tuberosum) cell cultures growing in control or limiting phosphate conditions. Sugar uptake was quantified using [U-14C]sucrose as precursor. Carbohydrate degradation through glycolysis and respiratory pathways was estimated using the catabolism of [U-14C]sucrose to 14CO2. Anaplerotic carbon flux was assessed by labeling with NaH14CO3. The data showed that these metabolic fluxes displayed distinct patterns over culture time. However, phosphate depletion had relatively little impact on the various fluxes. Sucrose uptake was higher during the first six days of culture, followed by a decline, which was steeper in Pi-sufficient cells. Anaplerotic pathway flux was more important at day three and decreased thereafter. In contrast, the flux between sucrose and CO2 was at a maximum in the mid-log phase of the culture, with a peak at Day 6. Metabolization of [U-14C]sucrose into neutral, basic and acidic fractions was also unaffected by phosphate nutrition. Hence, the well-documented changes in central metabolism enzymes activities in response to Pi deficiency do not drastically modify metabolic fluxes, but rather result in the maintenance of the carbon fluxes that support respiration.

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Section: Chemicals and Plant Materialsmentioning

confidence: 99%

Section: Sugar Catabolic Fluxes Are Similar In +Pi and −Pi Cellsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Carbon Fluxes in Potato (Solanum tuberosum) Remain Stable in Cell Cultures Exposed to Nutritional Phosphate Deficiency

He,

Dorion,

Carmona-Rojas

et al. 2023

Biology

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Tracing metabolic flux through time and space with isotope labeling experiments

Allen

Young

2020

Current Opinion in Biotechnology

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A phosphatase gene is linked to nectar dihydroxyacetone accumulation in mānuka (Leptospermum scoparium)

Grierson,

Thrimawithana,

van Klink

et al. 2024

New Phytologist

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Summary Floral nectar composition beyond common sugars shows great diversity but contributing genetic factors are generally unknown. Mānuka (Leptospermum scoparium) is renowned for the antimicrobial compound methylglyoxal in its derived honey, which originates from the precursor, dihydroxyacetone (DHA), accumulating in the nectar. Although this nectar trait is highly variable, genetic contribution to the trait is unclear. Therefore, we investigated key gene(s) and genomic regions underpinning this trait. We used RNAseq analysis to identify nectary‐associated genes differentially expressed between high and low nectar DHA genotypes. We also used a mānuka high‐density linkage map and quantitative trait loci (QTL) mapping population, supported by an improved genome assembly, to reveal genetic regions associated with nectar DHA content. Expression and QTL analyses both pointed to the involvement of a phosphatase gene, LsSgpp2. The expression pattern of LsSgpp2 correlated with nectar DHA accumulation, and it co‐located with a QTL on chromosome 4. The identification of three QTLs, some of the first reported for a plant nectar trait, indicates polygenic control of DHA content. We have established plant genetics as a key influence on DHA accumulation. The data suggest the hypothesis of LsSGPP2 releasing DHA from DHA‐phosphate and variability in LsSgpp2 gene expression contributing to the trait variability.

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Sustained substrate cycles between hexose phosphates and free sugars in phosphate-deficient potato (Solanum tuberosum) cell cultures

Cited by 3 publications

References 96 publications

Carbon Fluxes in Potato (Solanum tuberosum) Remain Stable in Cell Cultures Exposed to Nutritional Phosphate Deficiency

Carbon Fluxes in Potato (Solanum tuberosum) Remain Stable in Cell Cultures Exposed to Nutritional Phosphate Deficiency

Tracing metabolic flux through time and space with isotope labeling experiments

A phosphatase gene is linked to nectar dihydroxyacetone accumulation in mānuka (Leptospermum scoparium)

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