Sutterella wadsworthensis gen. nov., sp. nov., Bile-Resistant Microaerophilic Campylobacter gracilis-Like Clinical Isolates

Wexler, Hannah M.; Reeves, Denise; Summanen, Paula; Molitoris, E; McTeague, M.; Duncan, J F; Wilson, Kenneth H.; Finegold, Sydney M.

doi:10.1099/00207713-46-1-252

Cited by 81 publications

(41 citation statements)

References 6 publications

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“…Similarly, a ''C. gracilis-like'' organism from intestinal tract infections was assigned to a new genus as Sutterella wadsworthensis (Wexler et al 1996).…”

Section: Developments In Taxonomymentioning

confidence: 99%

The Family Campylobacteraceae

Lastovica¹,

On²,

Zhang³

2014

The Prokaryotes

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“…Similarly, a ''C. gracilis-like'' organism from intestinal tract infections was assigned to a new genus as Sutterella wadsworthensis (Wexler et al 1996).…”

Section: Developments In Taxonomymentioning

confidence: 99%

The Family Campylobacteraceae

Lastovica¹,

On²,

Zhang³

2014

The Prokaryotes

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“…Long-chain cellular fatty acids were detected with a Hewlett-Packard 5890 series II gas chromatograph, and cellular fatty acid profiles were determined by the Microbial Identification System software (MIDI, Newark, N.J.) as described previously (13). The corresponding library (ANAER-OBE, version 5.0) was used in successive analyses.…”

Section: Methodsmentioning

confidence: 99%

“ Bacteroides goldsteinii sp. nov.” Isolated from Clinical Specimens of Human Intestinal Origin

Song¹,

Liu²,

Lee³

et al. 2005

J Clin Microbiol

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Phenotypic and phylogenetic studies were performed on an unknown gram-negative, strictly anaerobic, rod-shaped bacterium isolated from human clinical specimens. This organism was indole negative, resistant to 20% bile, produced acetic and a lesser amount of succinic acids as the major end products of glucose metabolism, and possessed a G؉C content of approximately 43 mol%. Comparative 16S rRNA gene sequencing demonstrated that the unidentified bacterium was a member of the Cytophaga-Flavobacter-Bacteroides phylum of gram-negative bacteria and formed a close association (with an average sequence similarity of 93.6%) with the second subcluster of the Porphyromonas cluster in the Bacteroides subgroup. Phylogenetically and phenotypically it resembled Bacteroides merdae; however, a 16S rRNA gene sequence divergence of approximately 5.5% between the unknown bacterium and B. merdae, as well as distinguishable biochemical characteristics, demonstrate that the unknown bacterium is genotypically and phenotypically distinct and represents a previously unknown subline within the Porphyromonas phylogenetic cluster. The taxonomy of Bacteroides has undergone significant changes in the past few years (8). Studies have shown that the genus Bacteroides contained species representing several genera. A majority of the species previously included in the genus Bacteroides have been placed in the genera Porphyromonas, Prevotella, and Bacteroides sensu stricto (9-11). Several other genera have subsequently been described for Bacteroides species which do not conform to these three major groups (e.g., Anaerorhabdus, Dichelobacter, Dialister, Fibrobacter, Megamonas, Mitsuokella, Rikenella, Sebaldella, Tannerella, Tissierella, and Alistipes). The taxonomic positions of some other species still included in the genus, such as Bacteroides distasonis and Bacteroides merdae, remain uncertain; all of these species will ultimately be transferred to other genera (6). Furthermore, several clinically important species still await formal description.In this paper, we report on the characterization of a group of isolates which were recovered from clinical specimens of human intestinal origin. Phenotypically, the unknown bacterium is very much like B. merdae; these strains were misidentified as B. merdae previously. However, 16S rRNA sequencing reveals approximately 5.5% sequence divergence between the novel species and its phylogenetically closest species, B. merdae. A DNA-DNA hybridization study also confirmed that this unknown organism was indeed distinct from its nearest valid species, B. merdae. Based on the phenotypic and phylogenetic findings presented here, a new species, "Bacteroides goldsteinii," is proposed. In addition, we describe the phenotypic tests useful in distinguishing between this novel organism and its related taxa. (Table 1). The novel isolates were recovered from clinical sources, such as peritoneal fluid, appendix tissue, and intra-abdominal abscess; therefore, they are likely of intestinal origin. All the clinical isolates of...

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“…Remarkably, the clones that clustered in the Bacteroides phylum were isolated only from the dead fraction; one clone (AD8) was affiliated with the Bacteroides vulgatus subgroup, and another clone (AD9) was affiliated with the Bacteroides distasonis subgroup. Finally, one clone that was closely related to the gram-negative bacterium Sutterella wadsworthensis (99% sequence similarity), which is known to be associated with intestinal infections, was recovered from the live fecal cells (51).…”

Section: Vol 71 2005mentioning

confidence: 99%

Genetic Diversity of Viable, Injured, and Dead Fecal Bacteria Assessed by Fluorescence-Activated Cell Sorting and 16S rRNA Gene Analysis

Ben-Amor

Heilig

Smidt

et al. 2005

Appl Environ Microbiol

188

118

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A novel approach combining a flow cytometric in situ viability assay with 16S rRNA gene analysis was used to study the relationship between diversity and activity of the fecal microbiota. Simultaneous staining with propidium iodide (PI) and SYTO BC provided clear discrimination between intact cells (49%), injured or damaged cells (19%), and dead cells (32%). The three subpopulations were sorted and characterized by denaturing gradient gel electrophoresis (DGGE) of 16S rRNA gene amplicons obtained from the total and bifidobacterial communities. This analysis revealed that not only the total community but also the distinct subpopulations are characteristic for each individual. Cloning and sequencing of the dominant bands of the DGGE patterns showed that most of clones retrieved from the live, injured, and dead fractions belonged to Clostridium coccoides, Clostridium leptum, and Bacteroides. We found that some of the butyrate-producing related bacteria, such as Eubacterium rectale and Eubacterium hallii, were obviously viable at the time of sampling. However, amplicons affiliated with Bacteroides and Ruminococcus obeum-and Eubacterium biforme-like bacteria, as well as Butyrivibrio crossotus, were obtained especially from the dead population. Furthermore, some bacterial clones were recovered from all sorted fractions, and this was especially noticeable for the Clostridium leptum cluster. The bifidobacterial phylotypes identified in total samples and sorted fractions were assigned to Bifidobacterium adolescentis, Bifidobacterium longum, Bifidobacterium infantis, Bifidobacterium pseudocatenulatum, and Bifidobacterium bifidum. Phylogenetic analysis of the live, dead, and injured cells revealed a remarkable physiological heterogeneity within these bacterial populations; B. longum and B. infantis were retrieved from all sorted fractions, while B. adolescentis was recovered mostly from the sorted dead fraction.

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Sutterella wadsworthensis gen. nov., sp. nov., Bile-Resistant Microaerophilic Campylobacter gracilis-Like Clinical Isolates

Cited by 81 publications

References 6 publications

The Family Campylobacteraceae

The Family Campylobacteraceae

“ Bacteroides goldsteinii sp. nov.” Isolated from Clinical Specimens of Human Intestinal Origin

Genetic Diversity of Viable, Injured, and Dead Fecal Bacteria Assessed by Fluorescence-Activated Cell Sorting and 16S rRNA Gene Analysis

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