Sutureless transplantation of in vivo priming human mesenchymal stem cell sheet promotes the therapeutic potential for cardiac repair

Choi, Andrew; Kim, Hyeok; Han, Hyeonseok; Park, Jae-Hyun; Kim, Jin Ju; Sim, Woo‐Sup; Lee, Seong Jin; Ban, Kiwon; Park, Hun‐Jun; Kim, Dong Sung

doi:10.1088/1758-5090/ac8dc9

Cited by 11 publications

(8 citation statements)

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“…Elastin plays an important role in the stimulation of cell signaling cascades associated with proliferation and angiogenesis [ 274 ]. Elastin-like recombinamers (ELRs) are bio-engineered polypeptides with pentapeptide (VPGXG) repeat, where X can be any amino acid excluding proline [ 275 ].…”

Section: Induction Of Angiogenesis Using Scaffolds For Muscle Regener...mentioning

confidence: 99%

Tissue engineering modalities in skeletal muscles: focus on angiogenesis and immunomodulation properties

et al. 2023

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Muscular diseases and injuries are challenging issues in human medicine, resulting in physical disability. The advent of tissue engineering approaches has paved the way for the restoration and regeneration of injured muscle tissues along with available conventional therapies. Despite recent advances in the fabrication, synthesis, and application of hydrogels in terms of muscle tissue, there is a long way to find appropriate hydrogel types in patients with congenital and/or acquired musculoskeletal injuries. Regarding specific muscular tissue microenvironments, the applied hydrogels should provide a suitable platform for the activation of endogenous reparative mechanisms and concurrently deliver transplanting cells and therapeutics into the injured sites. Here, we aimed to highlight recent advances in muscle tissue engineering with a focus on recent strategies related to the regulation of vascularization and immune system response at the site of injury.

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Section: Induction Of Angiogenesis Using Scaffolds For Muscle Regener...mentioning

confidence: 99%

Tissue engineering modalities in skeletal muscles: focus on angiogenesis and immunomodulation properties

et al. 2023

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“…In addition, transplantation of BM-MSC sheets in a model of rat MI enhanced initial retention and amplified subsequent paracrine effects to recover damaged heart, compared with intramyocardial injection [74]. In one study, researchers showed that the sutureless transplantation of aligned a BMSC/HUVEC stacked sheet enhancing the in vivo priming, reduced the fibrosis, and increased the angiogenesis in the injured heart [75]. Miyahara and colleagues evaluated the therapeutic effects of autologous transplantation of single-layer adipose tissue-derived MSCs sheets in a MI rat model.…”

Section: Mscs-based Cell Sheetsmentioning

confidence: 99%

Progress and promise of cell sheet assisted cardiac tissue engineering in regenerative medicine

et al. 2023

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Cardiovascular diseases (CVDs) are the most common leading causes of premature deaths in all countries. To control the harmful side effects of CVDs on public health, it is necessary to understand the current and prospective strategies in prevention, management, and monitoring CVDs. In vitro, recapitulating of cardiac complex structure with its various cell types is a challenging topic in tissue engineering. Cardiac tissue engineering (CTE) is a multi-disciplinary strategy that has been considered as a novel alternative approach for cardiac regenerative medicine and replacement therapies. In this review, we overview various cell types and approaches in cardiac regenerative medicine. Then, the applications of cell-sheet-assisted CTE in cardiac diseases were discussed. Finally, we described how this technology can improve cardiac regeneration and function in preclinical and clinical models.

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“…The left ventricular internal dimension at end-diastole (LVIDd) and left ventricular internal dimension at endsystole (LVIDs) were measured using the two-dimensional (2D) targeted M-mode to calculate the left ventricular ejection fraction (LVEF) and left ventricular fractional shortening (LVFS). LVEF and LVFS were calculated using the following equations [7,9,31,35]:…”

Section: Echocardiographymentioning

confidence: 99%

Transplantation of stem cell spheroid-laden three-dimensional patches with bioadhesives for the treatment of myocardial infarction.

Jeon,

Kang,

Bhang

et al. 2023

Preprint

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Background Myocardial infarction (MI) is treated with stem cell transplantation using various methods. However, current methods, such as stem cell/spheroids injection, cell sheets, and cardiac patches have some limitations, such as low stem cell engraftment and poor therapeutic effects. Furthermore, these methods cause secondary damage due to injection and suturing to immobilize them in the heart, inducing side effects. Methods We fabricated human adipose-derived stem cell spheroids-laden three-dimensional (3D)-printed patches. The morphology, viability, and paracrine angiogenic effect of spheroids formed within 3D patches were analyzed in in vitro experiments. We synthesized thiolated gelatin and maleimide-conjugated gelatin as the polymers and fabricated a tissue adhesive biosealant using the polymers. The biocompatibility and biodegradability of the biosealant were evaluated using human dermal fibroblasts in vitro and the mouse subcutaneous models in vivo. In addition, the therapeutic effects of stem cell spheroid-laden 3D patches (S_3DP) with biosealant were evaluated using a rat MI model in vivo. Results The spheroids showed increased viability and expression of angiogenic factors compared to two-dimensional cultured cells. Our gelatin-based tissue adhesive biosealants were rapidly formed via a thiol-ene reaction and disulfide bond formation and revealed stronger tissue adhesiveness than commercial fibrin glue. Furthermore, we successfully applied S_3DP using a biosealant in a rat MI model without suturing in vivo, thereby improving cardiac function and reducing fibrosis of the heart. Conclusion We developed S_3DP with gelatin-based tissue adhesive biosealant to treat MI. This 3D patch has dual modules, such as open pockets to directly deliver the spheroids with their paracrine effects and closed pockets to improve the engraft rate by protecting the spheroid from harsh microenvironments. In summary, S_3DP and biosealant have excellent potential as advanced stem cell therapies with a sutureless approach to MI treatment.

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Sutureless transplantation of in vivo priming human mesenchymal stem cell sheet promotes the therapeutic potential for cardiac repair

Cited by 11 publications

References 50 publications

Tissue engineering modalities in skeletal muscles: focus on angiogenesis and immunomodulation properties

Tissue engineering modalities in skeletal muscles: focus on angiogenesis and immunomodulation properties

Progress and promise of cell sheet assisted cardiac tissue engineering in regenerative medicine

Transplantation of stem cell spheroid-laden three-dimensional patches with bioadhesives for the treatment of myocardial infarction.

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