SV-IV, a major protein secreted from rat seminal vesicle epithelium, promotes lymphocyte cytotoxic activity against the lymphoblastoid Raji cell line in human peripheral blood mononuclear cells

Peluso, Gianfranco; Marchese, Magda; Furgi, Annita; Ranieri, Marilena; Spena, Silvana Russo; Ravagnan, Giampietro; Fuggetta, Maria Pia; Porta, Raffaele; Metafora, Vittoria; Metafora, Salvatore

doi:10.1002/(sici)1097-0215(19970717)72:2<321::aid-ijc20>3.0.co;2-h

Cited by 6 publications

(2 citation statements)

References 18 publications

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“…Similar results were obtained when K562 or Daudi cells were used as target (manuscript in preparation). The reduced susceptibility to lysis of SV‐IV‐treated cells may be related to the ability of SV‐IV to bind to their plasma membranes (see above) with subsequent inhibition of either surface cytotoxicity target structures or apoptosis‐inducing cytotoxicity mechanisms (Peluso et al, 1997).…”

Section: Resultsmentioning

confidence: 99%

The immunomodulatory protein SV‐IV protects serum‐deprived cells against apoptosis but not against G0/G1 arrest: Possible implications for the survival of implanting embryo

Morelli

Peluso

Petillo

et al. 2007

Journal Cellular Physiology

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Serum deprivation induced in human lymphoblastoid Raji cells oxidative stress-associated apoptotic death and G0/G1 cell cycle arrest. Addition into culture medium of the immunomodulatory protein Seminal vesicle protein 4 (SV-IV) protected these cells against apoptosis but not against cycle arrest. The antiapoptotic activity was related to: (1) decrease of endocellular reactive Oxygen species (ROS) (2) increase of mRNAs encoding anti-oxidant enzymes (catalase, G6PD) and antiapoptotic proteins (survivin, cox-1, Hsp70, c-Fos); (3) decrease of mRNAs encoding proapoptotic proteins (c-myc, Bax, caspase-3, Apaf-1). The biochemical changes underlaying these effects were probably induced by a protein tyrosine kinase (PTK) activity triggered by the binding of SV-IV to its putative plasma membrane receptors. The ineffectiveness of SV-IV to abrogate the cycle arrest was accounted for by its downregulating effects on D1,3/E G1-cyclins and CdK2/4 gene expression, ppRb/pRb ratio, and intracellular ROS concentration. In conclusion, these experiments: (1) prove that SV-IV acts as a cell survival factor; (2) suggest the involvement of a PTK in SV-IV signaling; (3) point to cell cycle-linked enzyme inhibition as responsible for cycle arrest; (4) provide a model to dissect the cycle arrest and apoptosis induced by serum withdrawal; (5) imply a possible role of SV-IV in the survival of hemiallogenic implanting embryos.

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Section: Resultsmentioning

confidence: 99%

The immunomodulatory protein SV‐IV protects serum‐deprived cells against apoptosis but not against G0/G1 arrest: Possible implications for the survival of implanting embryo

Morelli

Peluso

Petillo

et al. 2007

Journal Cellular Physiology

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“…First, as patients at high risk of asynchrony (for example, difficult-to-wean or severe COPD patients) were not specifically selected in this study [7] and because we targeted a V T of 6 to 8 ml/kg in level 100 [40], a very low incidence of asynchrony was observed with all modes and conditions. This study may therefore have underestimated the benefits of NAVA and PAV [20,39,41,42], but we deliberately decided to compare these modes in patients in the recovery phase after acute respiratory failure encountered in daily practice rather than in a very selected population, with the risk of showing results that would be transposable only to a niche population.…”

Section: Discussionmentioning

confidence: 99%

Neurally adjusted ventilatory assist and proportional assist ventilation both improve patient-ventilator interaction

et al. 2015

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IntroductionThe objective was to compare the impact of three assistance levels of different modes of mechanical ventilation; neurally adjusted ventilatory assist (NAVA), proportional assist ventilation (PAV), and pressure support ventilation (PSV) on major features of patient-ventilator interaction.MethodsPSV, NAVA, and PAV were set to obtain a tidal volume (VT) of 6 to 8 ml/kg (PSV100, NAVA100, and PAV100) in 16 intubated patients. Assistance was further decreased by 50% (PSV50, NAVA50, and PAV50) and then increased by 50% (PSV150, NAVA150, and PAV150) with all modes. The three modes were randomly applied. Airway flow and pressure, electrical activity of the diaphragm (EAdi), and blood gases were measured. VT, peak EAdi, coefficient of variation of VT and EAdi, and the prevalence of the main patient-ventilator asynchronies were calculated.ResultsPAV and NAVA prevented the increase of VT with high levels of assistance (median 7.4 (interquartile range (IQR) 5.7 to 10.1) ml/kg and 7.4 (IQR, 5.9 to 10.5) ml/kg with PAV150 and NAVA150 versus 10.9 (IQR, 8.9 to 12.0) ml/kg with PSV150, P <0.05). EAdi was higher with PAV than with PSV at level100 and level150. The coefficient of variation of VT was higher with NAVA and PAV (19 (IQR, 14 to 31)% and 21 (IQR 16 to 29)% with NAVA100 and PAV100 versus 13 (IQR 11 to 18)% with PSV100, P <0.05). The prevalence of ineffective triggering was lower with PAV and NAVA than with PSV (P <0.05), but the prevalence of double triggering was higher with NAVA than with PAV and PSV (P <0.05).ConclusionsPAV and NAVA both prevent overdistention, improve neuromechanical coupling, restore the variability of the breathing pattern, and decrease patient-ventilator asynchrony in fairly similar ways compared with PSV. Further studies are needed to evaluate the possible clinical benefits of NAVA and PAV on clinical outcomes.Trial registrationClinicaltrials.gov NCT02056093. Registered 18 December 2013.Electronic supplementary materialThe online version of this article (doi:10.1186/s13054-015-0763-6) contains supplementary material, which is available to authorized users.

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Identification of rat prostatic steroid binding protein (PSBP) as an immunosuppressive factor

Maccioni¹,

Riera²,

Rivero³

2001

Journal of Reproductive Immunology

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SV-IV, a major protein secreted from rat seminal vesicle epithelium, promotes lymphocyte cytotoxic activity against the lymphoblastoid Raji cell line in human peripheral blood mononuclear cells

Cited by 6 publications

References 18 publications

The immunomodulatory protein SV‐IV protects serum‐deprived cells against apoptosis but not against G0/G1 arrest: Possible implications for the survival of implanting embryo

The immunomodulatory protein SV‐IV protects serum‐deprived cells against apoptosis but not against G0/G1 arrest: Possible implications for the survival of implanting embryo

Neurally adjusted ventilatory assist and proportional assist ventilation both improve patient-ventilator interaction

Identification of rat prostatic steroid binding protein (PSBP) as an immunosuppressive factor

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