Sv40‐induced immortalization and <i>ras</i>‐transformation of human bronchial epithelial cells

Reddel, Roger R.; Silva, R. De; Duncan, Emma L.; Rogan, Eileen M.; Whitaker, Noel J.; Zahra, David; Ke, Yang; McMenamin, Mary G.; Gerwin, Brenda I.; Harris, Curtis C.

doi:10.1002/ijc.2910610210

Cited by 41 publications

(21 citation statements)

References 20 publications

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“…In contrast, a few previous studies have shown that activated ras can alter the transplantability of BEAS-2B (Amstad et al, 1988;Reddel et al, 1988bReddel et al, , 1995. These findings suggest that in these previous experiments c-jun expression may have been constitutively sustained during the selection of the activated K-ras-transfected stable clones.…”

Section: Discussioncontrasting

confidence: 61%

Altered regulation of c-jun and its involvement in anchorage-independent growth of human lung cancers

et al. 2005

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Section: Discussioncontrasting

confidence: 61%

Altered regulation of c-jun and its involvement in anchorage-independent growth of human lung cancers

et al. 2005

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“…IHC and Western blot experiments were performed on BEAS-2B cells ( Figure 5) (Ke et al 1988;Reddel et al 1995). In Figure 5A, BEAS-2B cells were grown on chamber slides, fixed and stained with RACK1 (red), and mounted with Vectashield containing DAPI.…”

Section: Resultsmentioning

confidence: 99%

RACK1, a PKC Targeting Protein, Is Exclusively Localized to Basal Airway Epithelial Cells

Slager

DeVasure

Pavlik

et al. 2007

J Histochem Cytochem.

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The novel isoform of protein kinase C (PKC), PKC∊, is an important regulator of ciliated cell function in airway epithelial cells, including cilia motility and detachment of ciliated cells after environmental insult. However, the mechanism of PKC∊ signaling in the airways and the potential role of the PKC∊-interacting protein, receptor for activated C kinase 1 (RACK1), has not been widely explored. We used immunohistochemistry and Western blot analysis to show that RACK1 is localized exclusively to basal, non-ciliated (and non-goblet) bovine and human bronchial epithelial cells. Our immunohistochemistry experiments used the basal body marker pericentrin, a marker for cilia, β-tubulin, and an airway goblet cell marker, MUC5AC, to confirm that RACK1 was excluded from differentiated airway cell subtypes and is only expressed in the basal cells. These results suggest that PKC∊ signaling in the basal airway cell may involve RACK1; however, PKC∊ regulation in ciliated cells uses RACK1-independent pathways.

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“…To investigate the molecular mechanism underlying the malignant progression of these tumors, particularly SCLC, we have recently done studies to identify possible candidate genes involved in tumorigenicity and metastasis of SCLC cells. Using microquantity differential display (MDD) developed in our laboratory (10), we have compared the gene expression profiles between the normal bronchial epithelial cell line Beas-2b (11) and a malignant SCLC cell line Lu-165 (12). A cDNA fragment was identified to be up-regulated in Lu-165 cells.…”

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confidence: 99%

Increased Expression of Id Family Proteins in Small Cell Lung Cancer and its Prognostic Significance

Kamalian

Gosney

Forootan

et al. 2008

Clinical Cancer Research

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Purpose: To study the molecular pathology of human small cell lung cancer (SCLC), molecular biology approaches were used to identify genes involved in malignant progression of the cancer cells. Experimental Design: Microquantity differential display was used initially to identify genes expressed differentially between normal and malignant cell lines. The differences were verified by Western blot. Immunohistochemical analysis was done on paired normal and malignant lung tissues and on tissues taken by biopsy to assess the expression status of candidate genes and their prognostic significance. Results: Inhibitor of DNA/differentiation (Id)1 gene was up-regulated in SCLC cells. Levels of Id1in 8 of 10 cell lines were increased by 1.7-to 21.4-fold when compared with the benign cells. A similar increase was also found in levels of Id2 and Id3. On 26 pairs of lung tissues, all four Id proteins were significantly (Wilcoxon Signed Rank Test, P < 0.001-0.005) overexpressed in cytoplasm of the malignant cells. In nuclei of SCLC cells, Id1 expression was significantly reduced, whereas the levels of Id2, Id3, and Id4 were significantly (Wilcoxon Signed Rank Test, P < 0.001) increased. Immunohistochemical staining on biopsy specimens showed that the increased expression of Id2 in cytoplasm of cancer cells, not the other three proteins, was significantly associated with the increased survival of SCLC patients. Conclusion: Changed expression profiles of Id proteins may play important roles in malignant progression of SCLC, and the increased Id2 in cytoplasm is a novel prognostic factor to predict the patient outcomes.

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Sv40‐induced immortalization and ras‐transformation of human bronchial epithelial cells

Cited by 41 publications

References 20 publications

Altered regulation of c-jun and its involvement in anchorage-independent growth of human lung cancers

Altered regulation of c-jun and its involvement in anchorage-independent growth of human lung cancers

RACK1, a PKC Targeting Protein, Is Exclusively Localized to Basal Airway Epithelial Cells

Increased Expression of Id Family Proteins in Small Cell Lung Cancer and its Prognostic Significance

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