SVP‑B5 peptide from Buthus?martensii Karsch scorpion venom exerts hyperproliferative effects on irradiated hematopoietic cells

Abstract

Abstract. Previous studies have demonstrated the radioprotective efficacy of scorpion venom peptide, fraction II (SVPII) from the venom of Buthus martensii Karsch. In the present study, the SVP-B5 polypeptide, which is one of the active components of SVPII, was purified using a two-step chromatographic process. SVP-B5 significantly promoted the proliferation of irradiated M-NFS-60 mouse-derived myelocytic leukemia cells. In addition, SVP-B5 effectively and persistently promoted hematopoietic recovery and expansion of hematopoietic cells after irradiation as demonstrated by cobblestone area forming cell and long-term bone marrow culture assays. Treatment of M-NFS-60 cells with SVP-B5 upregulated the expression of interleukin 3 receptor and activated the Janus kinase-2/signal transducer and activator of transcription 5 signaling pathway. In conclusion, the present study demonstrated that SVP-B5 has growth factor-like properties and may be used as a therapeutic modality in the recovery of severe myelosuppression, which is a common side effect of radiotherapy.

Structural characterization and anti-inflammatory potency of Mesobuthus martensii Karsch oligopeptides in lipopolysaccharide (LPS)-induced RAW264.7 macrophages

“…24828 | RSC Adv., 2019, 9,[24822][24823][24824][24825][24826][24827][24828][24829][24830][24831][24832] This journal is © The Royal Society of Chemistry 2019 the production of pro-inammatory cytokines, the TNF-a, IL-6, and IL-1b levels in the culture media were measured using appropriate assay kits. Compared with the results for control groups, the TNF-a, IL-6, and IL-1b levels markedly increased by 20.3, 14.5 and 10.1fold, respectively (Fig.…”

“…7,8,18,23 The Chinese scorpion venom is a famous traditional Chinese medicine that has been widely used to treat paralysis, apoplexy, epilepsy and inammation. 9 Studies have reported that the scorpion venom peptides show a diverse array of biological activities, which are involved in immune, nervous, cardiovascular, and neoplastic diseases. Due to their structural and functional diversities, it has been proposed that scorpionderived peptides can be used to develop new specic drugs.…”