Swarming Differentiation and Swimming Motility in<i>Bacillus subtilis</i>Are Controlled by<i>swrA</i>, a Newly Identified Dicistronic Operon

Calvio, Cinzia; Celandroni, Francesco; Ghelardi, Emilia; Amati, Giuseppe; Salvetti, Sara; Ceciliani, Fabrizio; Galizzi, Alessandro; Senesi, Sonia

doi:10.1128/jb.187.15.5356-5366.2005

Cited by 110 publications

(121 citation statements)

References 40 publications

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“…swrA controls flagellar number and is indirectly responsible for swarming motility on semi-solid surfaces (Calvio et al, 2005;Patrick and Kearns, 2008). However, NAFIS1 (swrA::IS4Bsu1) and NAFM46 (swrA::Spc r ) cells did not show any defect in motility and spread on the agar plate as well as the parental strain (Fig.…”

Section: Discussionmentioning

confidence: 97%

“…swrA has no significant homology to known proteins and little is known about its function in regulating γPGA synthesis (Calvio et al, 2005;Osera et al, 2009;Stanley and Lazazzera, 2005). swrA controls flagellar number and is indirectly responsible for swarming motility on semi-solid surfaces (Calvio et al, 2005;Patrick and Kearns, 2008).…”

Section: Discussionmentioning

confidence: 99%

“…We named this mutant NAFIS1. swrA encodes a soluble cytoplasmic protein required for swarming motility and control of flagellar number (Calvio et al, 2005;Patrick and Kearns, 2008). swrA was also identified as a frameshift allele that confers a γPGA-negative phenotype to the laboratory strain B. subtilis 168 (Stanley and Lazazzera, 2005).…”

Section: Methodsmentioning

confidence: 99%

“…In this mutant, swrA, originally found as an allele that confers a γPGA-negative phenotype in the laboratory strain of B. subtilis (Stanley and Lazazzera, 2005), was interrupted by IS4Bsu1. Downstream of swrA, B. subtilis possesses minJ, which is a topological determinant of cell division (Calvio et al, 2005;Patrick and Kearns, 2008). minJ is reportedly co-transcribed with swrA (Calvio et al, 2005); however, results denying cistronic regulation of swrA-minJ have also been published (Patrick and Kearns, 2008).…”

Section: Introductionmentioning

confidence: 98%

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Loss of poly-.GAMMA.-glutamic Acid Synthesis of Bacillus subtilis (natto) Due to IS4Bsu1 Translocation to swrA Gene

Kimura

Tran²,

Funane

2011

FSTR

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The mobile genetic element IS4Bsu1 of Bacillus subtilis (natto), the commercial strain used for natto fermentation, was found to spontaneously translocate to the swrA gene, which led to a defect in poly-γ-glutamic acid (γPGA) synthesis. The γPGA defect of NAFIS1 (swrA::IS4Bsu1) was restored by a plasmid harboring the swrA gene. The insertion of IS4Bsu1 to the swrA gene appeared not to interfere with minJ gene expression, located downstream of the swrA gene, because a defect in cell motility exhibited by NAF16 (minJ::Spc r ) was not observed in NAFIS1 (swrA::IS4Bsu1). We found that the minJ gene is essential for γPGA synthesis.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

See 2 more Smart Citations

Loss of poly-.GAMMA.-glutamic Acid Synthesis of Bacillus subtilis (natto) Due to IS4Bsu1 Translocation to swrA Gene

Kimura

Tran²,

Funane

2011

FSTR

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“…Besides a genetic control (swr operon) swarming could also be modulated by lipopeptides production (Calvio et al, 2005). Surfactin mutants lost the ability in swarming and spreading on surfaces (Kinsinger et al, 2003).…”

Section: Motility Assays Of Mht6 and Mht88mentioning

confidence: 99%

Improvement of Antagonistic Activity of Bacillus megaterium MHT6 against Fusarium moniliforme using He-Ne Laser Irradiation

Fu¹,

Feng²,

YaNan³

et al. 2015

IJAB

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Corn ear rot has been the most important disease of the world. It is mainly caused by Fusarium moniliforme which produce a number of mycotoxins including Fumonisin B1 (FB1). Aiming at supplying a potentially effective biological control agent against F. moniliforme, Bacillus megaterium MHT6 isolated from China, was tested for its antagonistic activity against F. moniliforme. Moreover, both antifungal activity and the active compound of strain MHT6 were tested and identified. In order to improve its antifungal activity, a random mutagenesis generated using He-Ne laser irradiation was applied on B. megaterium MHT6. After the mutation, one hundred bacterial colonies were obtained and their effects against F. moniliforme were assessed in a plate assay. Eight colonies (8%) exhibited higher inhibition activity as compared to the wild type MHT6. Among them, the mutant MHT 88 which showed the strongest antagonistic ability was selected out. Through bacterial motility assays, the mutant MHT88 showed stronger swarming motility than wild type MHT6. According to FPLC and MALDI-TOF-MS, the production of two main antagonistic lipopeptides such as surfactin and fengycin increased in mutant MHT88. Especially the surfactin, which can't be detected in the wild strain MHT6, was obviously observed in mutant. Moreover, the cell-free supernatant of the mutant MHT88 showed stronger capability in degrading FB1 compared to MHT6. This study provided a promising biological agent to inhibit F. moniliforme and suppress corn ear rot disease.

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General and Regulatory Proteolysis in Bacillus subtilis

Molière

Turgay

2013

Subcellular Biochemistry

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The soil-dwelling bacterium Bacillus subtilis is widely used as a model organism to study the Gram-positive branch of Bacteria. A variety of different developmental pathways, such as endospore formation, genetic competence, motility, swarming and biofilm formation, have been studied in this organism. These processes are intricately connected and regulated by networks containing e.g. alternative sigma factors, two-component systems and other regulators. Importantly, in some of these regulatory networks the activity of important regulatory factors is controlled by proteases. Furthermore, together with chaperones, the same proteases constitute the cellular protein quality control (PQC) network, which plays a crucial role in protein homeostasis and stress tolerance of this organism. In this review, we will present the current knowledge on regulatory and general proteolysis in B. subtilis and discuss its involvement in developmental pathways and cellular stress management.

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Swarming Differentiation and Swimming Motility inBacillus subtilisAre Controlled byswrA, a Newly Identified Dicistronic Operon

Cited by 110 publications

References 40 publications

Loss of poly-.GAMMA.-glutamic Acid Synthesis of Bacillus subtilis (natto) Due to IS4Bsu1 Translocation to swrA Gene

Loss of poly-.GAMMA.-glutamic Acid Synthesis of Bacillus subtilis (natto) Due to IS4Bsu1 Translocation to swrA Gene

Improvement of Antagonistic Activity of Bacillus megaterium MHT6 against Fusarium moniliforme using He-Ne Laser Irradiation

General and Regulatory Proteolysis in Bacillus subtilis

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