Sweat testing for the diagnosis of cystic fibrosis: Practical considerations

LeGrys, Vicky A.

doi:10.1016/s0022-3476(96)70034-3

Cited by 132 publications

(93 citation statements)

References 14 publications

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“…Entretanto, é uma técnica trabalhosa que requer a determinação do peso exato do suor através de balança analítica e o cuidado para evitar a evaporação do mesmo durante a coleta, além de a amostra de suor necessitar ser submetida à análise bioquímica dos eletrólitos, técnica também relativamente complexa. Por esse método, deve-se colher um mínimo de 50 mg de suor 10,11 (idealmente ≥ 75 mg) para se obter um resultado acurado, o que pode ser difícil em lactentes menores de 1 a 2 meses. O procedimento torna-se suscetível a erros, podendo levar a resultados falso-positivos e falso-negativos caso não seja realizado por pessoal experiente e com treinamento especializado na coleta e análise do suor.…”

Section: Introductionunclassified

Comparison between classic Gibson and Cooke technique and sweat conductivity test in patients with and without cystic fibrosis

et al. 2010

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Objective: To compare sweat chloride values obtained by quantitative pilocarpine iontophoresis (classic test) with the sweat conductivity values obtained using Macroduct ® collection system in patients with and without cystic fibrosis (CF). The cost and time spent to carry out each test were also analyzed. Methods:The sweat test using both techniques was performed at the same time in patients with and without CF. Conductivity cutoff values to rule out or diagnose CF were < 75 and ≥ 90 mmol/L, respectively, and for the classic test the chloride values were < 60 and ≥ 60 mmol/L. Results:Fifty-two patients with CF (29 males and 23 females; aged from 1.5 to 18.2 years) underwent the sweat test using both techniques, showing median sweat chloride and conductivity values of 114 and 122 mmol/L, respectively. In all of them, conductivity was ≥ 95 mmol/L, which provided the test with 100% sensitivity (95%CI 93.1-100). Fifty patients without CF (24 males and 26 females; aged from 0.5 to 12.5 years) had median sweat chloride and conductivity values of 15.5 and 30 mmol/L, respectively. In all cases, conductivity was < 70 mmol/L, which provided the test with 100% specificity (95%CI 92.9-100). Time spent to perform the tests was significantly shorter for the conductivity test, and its cost was also lower. Conclusions:The conductivity test showed high sensitivity and specificity, and there was good correspondence between the tests. The time spent to carry out the conductivity test was shorter and the cost was lower in comparison with the classic test. J Pediatr (Rio J)

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Section: Introductionunclassified

Comparison between classic Gibson and Cooke technique and sweat conductivity test in patients with and without cystic fibrosis

et al. 2010

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“…Normal sweat contains less than 60 mmol/L chloride and sodium (Association of Clinical Biochemistry, 2002). The 60 mmol/L value of sweat chloride concentrations has been used for a long time to discriminate between the populations of patients with CF and without CF (LeGrys, 1996). An elevated sweat chloride level has been the "gold standard" for diagnosis of CF (Gibson & Cooke, 1959).…”

Section: Methods Used For Assessing Sweat Chloridementioning

confidence: 99%

Biochemical and Molecular Genetic Testing Used in the Diagnosis and Assessment of Cystic Fibrosis

McGrowder¹

2012

Cystic Fibrosis - Renewed Hopes Through Research

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“…For quality control purposes a significant discordance between the two electrolyte concentrations (>20 mmol/L) can indicate technical error in collection, analysis, or both. 3,5 We analyze aqueous electrolyte solutions such as N/2 and N/5 saline as internal quality controls in our laboratory with the patient's samples. Sodium can be measured by flame photometry, atomic absorption spectrophotometry and ion selective electrode technique.…”

Section: Case Reportmentioning

confidence: 99%

High sweat electrolytes in a malnourished child

Jasinge

Meeriyagalle

2011

J Diagnostic Path

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This case report describes an infant who presented with loose stools and none resolving respiratory tract infection and was found to have high sweat electrolyte levels. The sweat electrolyte concentrations declined during the course of treatment. This case report highlights the need to be aware of the false positive sweat test results that can occur in malnutrition and describes the important points in carrying out a proper sweat test.

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Sweat testing for the diagnosis of cystic fibrosis: Practical considerations

Cited by 132 publications

References 14 publications

Comparison between classic Gibson and Cooke technique and sweat conductivity test in patients with and without cystic fibrosis

Comparison between classic Gibson and Cooke technique and sweat conductivity test in patients with and without cystic fibrosis

Biochemical and Molecular Genetic Testing Used in the Diagnosis and Assessment of Cystic Fibrosis

High sweat electrolytes in a malnourished child

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