2021
DOI: 10.3390/ijms22020681
|View full text |Cite
|
Sign up to set email alerts
|

Sweet Basil Has Distinct Synthases for Eugenol Biosynthesis in Glandular Trichomes and Roots with Different Regulatory Mechanisms

Abstract: Production of a volatile phenylpropene; eugenol in sweet basil is mostly associated with peltate glandular trichomes (PGTs) found aerially. Currently only one eugenol synthase (EGS), ObEGS1 which belongs to PIP family is identified from sweet basil PGTs. Reports of the presence of eugenol in roots led us to analyse other EGSs in roots. We screened for all the PIP family reductase transcripts from the RNA-Seq data. In vivo functional characterization of all the genes in E. coli showed their ability to produce e… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1

Citation Types

2
9
0
1

Year Published

2021
2021
2024
2024

Publication Types

Select...
8
1

Relationship

0
9

Authors

Journals

citations
Cited by 11 publications
(12 citation statements)
references
References 58 publications
2
9
0
1
Order By: Relevance
“…DEGs were enriched to the “Plant-pathogen interaction” pathway, which is consistent with the conclusion that trichomes are related to the disease resistance of plants. We found that 6 out of the 10 pathways were related to the biosynthesis of secondary metabolites, which can help plants to adapt to the ever-changing external environment [ 46 ]. Transcriptome profiling of pepper was carried out to identify genes responsible for the formation of trichomes [ 47 ].…”
Section: Discussionmentioning
confidence: 99%
“…DEGs were enriched to the “Plant-pathogen interaction” pathway, which is consistent with the conclusion that trichomes are related to the disease resistance of plants. We found that 6 out of the 10 pathways were related to the biosynthesis of secondary metabolites, which can help plants to adapt to the ever-changing external environment [ 46 ]. Transcriptome profiling of pepper was carried out to identify genes responsible for the formation of trichomes [ 47 ].…”
Section: Discussionmentioning
confidence: 99%
“…The expression levels of the following genes were monitored with specific primer set of cinnamate 4-hydroxylase gene C4H (Accession No. HM990150) , 4-coumarate CoA ligase 4CL (KC576841) , cinnamoyl-CoA reductase CCR (in this study) , cinnamyl alcohol dehydrogenase CAD (AY879285) , coniferyl alcohol acetyl transferase CAAT1 (MN031888) , eugenol (and chavicol) synthase EGS1 (DQ372812) , eugenol O -methyltransferase EOMT (AF435008, XLOC_068107 and XLOC_068808) , 1,8-cineole synthase CIN (in this study) , S -linalool synthase LIS (in this study) , OPR3 ( O. basilicum Chr: scaffold10, 7,268,155–7,270,534) , and β-tubulin (MH620961.1) genes in O. basilicum (Supplementary Table 2 ) 15 18 . Relative expression ratios for each target gene were calculated using β-tubulin gene expression in the same experimental samples.…”
Section: Rna Expression Analysismentioning
confidence: 70%
“…According to the determined sequences, respective primer sets were synthesized in highly conserved regions for quantitative RT-PCR expression analysis of several paralogues at once. In addition, previously published basil genes 16 18 , cinnamate 4-hydroxilase ( C4H ), 4-coumaric acid coenzyme A ligase ( 4CL ), cinnamyl alcohol dehydrogenase ( CAD ), coniferyl alcohol acetyl transferase ( CAAT ) and eugenol synthases ( EGS ) primer sets could be used for this experiment.…”
Section: Effects Of N 2 O 5 Exp...mentioning
confidence: 99%
“…HM990150), 4-coumarate CoA ligase 4CL (KC576841), cinnamoyl-CoA reductase CCR2 (in this study), cinnamyl alcohol dehydrogenase CAD (AY879285), coniferyl alcohol acetyl transferase CAAT1 (MN031888), eugenol (and chavicol) synthase EGS1 (DQ372812), eugenol Omethyltransferase EOMT (AF435008, XLOC_068107 and XLOC_068808), 1,8-cineole synthase CIN (in this study), S-linalool synthase LIS (in this study), OPR3 (O. basilicum Chr: scaffold10, 7268155-7270534), and β-tubulin (MH620961.1) genes in O. basilicum (Supplementary Table 2). [49][50][51][52] Relative expression ratios for each target gene were calculated using β-tubulin gene expression in the same experimental samples. Basil CCR2, CIN and LIS cDNA sequences were determined by RNA sequencing analysis in this study (Supplementary Table 2).…”
Section: Rna Expression Analysismentioning
confidence: 99%