Sweetcorn husk derived porous carbon with inherent silica for ultrasensitive detection of ovarian cancer in blood plasma

Pandey, Ullas; Rani, Malothu Usha; Deshpande, Atul Suresh; Singh, Vikrant; Agrawal, Amit

doi:10.1016/j.electacta.2021.139258

Cited by 12 publications

(5 citation statements)

References 42 publications

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“…For the same, the Nernst–Plank relation is called, which explains how a flux of molecules travel in the electrolyte through a unit area of the electrode in a unit time interval due to diffusion, migration, and convection . Since a low concentration of electroactive species is used compared to the inert electrolyte (PBS), and it is also prevented from any stir and vibration, the case of migration and convection of electroactive species in the electrolyte stamps out . Thus, the nature of the mass transport is diffusive owing to a gradient in concentration.…”

Section: Resultsmentioning

confidence: 99%

“…Toward this, electrochemical biosensors built on the nanomaterial-based transduction, have emerged as a solution where rapid detection is possible with quantification capability and a very low detection limit . The surface modification strategies for bioreceptor functionalization lead to enhanced signal amplification because of its high specific surface area, porosity, and nanoscale tunability. − Among many nanoscale morphologies, the 3D structures especially nanoflowers offer very high surface area and more geometrical volume, aiding to the better loading of the bioreceptors. Considering the composition of the nanostructures, zinc oxide (ZnO) has been widely explored in the literature for biosensing applications.…”

Section: Introductionmentioning

confidence: 99%

“…Considering the composition of the nanostructures, zinc oxide (ZnO) has been widely explored in the literature for biosensing applications. ZnO nanostructures have several distinctive features such as good electrical transport, wide band gap, high isoelectric point, cost-effectiveness, nontoxicity, chemical stability, and high exciton binding energy, which renders it a perfect choice for electrochemical biosensing application. , Notably, there have been many reports for the development of electrochemical biosensors for estimating virus-borne disease, bacterial infraction, protein-biomarker identification, heavy metal detection, etc., in the controlled experimental samples. − However, the nanotechnology-assisted sensor fabrication protocol is associated with many probabilistic events, questioning its on-field applicability time and again. Furthermore, the clinical samples are associated with a much complex matrix than that of its control counterpart, thereby putting much more challenge to the near-perfect quantification …”

Section: Introductionmentioning

confidence: 99%

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ZnO Nanoflower-Based Electrochemical SARS-CoV-2 Molecular Biosensors with Improved Diagnostic Accuracy

Pandey,

Goswami,

Singh

2024

ACS Appl. Nano Mater.

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Despite the promising performance of nanomaterial-based electrochemical detection techniques, product-level pointof-care sensor systems are still evolving. Unique approaches toward nanoscale tunability, enriched measurements, and data analysis are therefore imperative. The recent outbreak of coronavirus disease provides a suitable test bench toward this pursuit, attracting several innovative efforts from the sensor research community. Accordingly, this work reports an ultrasensitive and selective molecular biosensor for RT-PCR-confirmed hospital samples of SARS-CoV-2 built on ZnO nanoflowers. The unique experimental and analytical methodologies drastically improve the device efficiency, relying on three conserved genomic sequences (E, N, and ORF1ab genes). It involves multiple techniques like cyclic voltammetry, electrochemical impedance spectroscopy, and differential pulse voltammetry where normalized responses of these techniques are used to create 1D, 2D, and 3D data points representing each sample for clustering both by heuristic (2D separation plane) and ML approach (logistic regression) for accurate diagnosis. Enhancing data features also enlarges the Euclidean distances (ED) of data points, which raises the separation efficiency of the device to 93.75%. As the 3D space also allows the data (with nearly identical EDs) to have different spatial orientations for efficient clustering, samples were dichotomized into positive and negative with 100% efficiency, sensitivity, and specificity. Furthermore, owing to the ultrasensitive nanostructured transducer, the viral load was successfully quantified in the spiked control samples with a commendable detection sensitivity and limits of detection as low as 14.137 fM (E-gene). The device's fast response time (<45 min) justifies its potential on-field applicability.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

ZnO Nanoflower-Based Electrochemical SARS-CoV-2 Molecular Biosensors with Improved Diagnostic Accuracy

Pandey,

Goswami,

Singh

2024

ACS Appl. Nano Mater.

Self Cite

View full text Add to dashboard Cite

show abstract

“…The EIS is a more sensitive technique for close examination of the charge transfer process at the interface of the bio-electrode which is very well established in our previous communications [39][40][41][42][43]. The magnitude of impedance in figure 3(C), the impedance increases monotonically with an increment in the concentration of MMP-2 protein added serially.…”

Section: Biosensing Of Mmp-2 Cancer Biomarkermentioning

confidence: 87%

2D vanadium disulfide nanosheets assisted ultrasensitive, rapid, and label-free electrochemical quantification of cancer biomarker (MMP-2)

2023

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Cancer is one of the most tormenting global health burdens reporting high mortality and morbidity worldwide. Matrix metalloproteinase 2 (MMP-2) protein has elevated expression for most types of cancers, including prostate and breast cancer. Therefore, accurate and specific detection of MMP-2 biomarker is crucial for screening, treatment, and prognosis of related cancer. In this work, we have proposed a label-free electrochemical biosensor for the detection of MMP-2 protein. This biosensor was fabricated using hydrothermally synthesized vanadium disulfide (VS2) nanosheets with monoclonal anti-MMP2 antibodies biofunctionalized using a suitable linker. The VS2 nanomaterials were synthesized hydrothermally at different reaction temperatures (140, 160, 180 and 200) generating different morphologies from a 3-D bulk cubic structure at 140C to 2-D nanosheets at 200C. Owing to the advantages of 2-D VS2 nanosheets with high surface-to-volume ratio, excellent electrochemical response and high antibody loading possibility, it was selected for fabricating an MMP-2 specific biosensor. The antibody-antigen binding event is analyzed by recording electrochemical impedance spectroscopy (EIS) signals for different target MMP-2 protein concentrations. The sensitivity and lower limit of detection (LOD) were 7.272 (R/R)(ng/mL)-1cm-2 and 0.138 fg/mL, respectively in 10 mM phosphate buffer saline (PBS) for this proposed sensor. Further, interference studies were also performed which demonstrates the sensor to be highly selective against non-specific target proteins. This 2-D VS2 nanosheet-based electrochemical biosensor is a sensitive, cost-effective, accurate, and selective solution for cancer diagnosis.

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“…Numerous fruit wastes, such as peels and husks from fruits like apples, bananas, oranges, pomelo, and dragon fruit, have been extensively studied for their potential to develop highly active electrochemical sensors [ 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 ]. In a study by Zhang et al [ 39 ], an electrochemical catalyst was fabricated to simultaneously detect ascorbic acid, dopamine, and uric acid using differential pulse voltammetry (DPV) analysis.…”

Section: Classification and Applicationsmentioning

confidence: 99%

Biomass-Derived Carbon-Based Electrodes for Electrochemical Sensing: A Review

Onfray,

Thiam

2023

Micromachines

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The diverse composition of biomass waste, with its varied chemical compounds of origin, holds substantial potential in developing low-cost carbon-based materials for electrochemical sensing applications across a wide range of compounds, including pharmaceuticals, dyes, and heavy metals. This review highlights the latest developments and explores the potential of these sustainable electrodes in electrochemical sensing. Using biomass sources, these electrodes offer a renewable and cost-effective route to fabricate carbon-based sensors. The carbonization process yields highly porous materials with large surface areas, providing a wide variety of functional groups and abundant active sites for analyte adsorption, thereby enhancing sensor sensitivity. The review classifies, summarizes, and analyses different treatments and synthesis of biomass-derived carbon materials from different sources, such as herbaceous, wood, animal and human wastes, and aquatic and industrial waste, used for the construction of electrochemical sensors over the last five years. Moreover, this review highlights various aspects including the source, synthesis parameters, strategies for improving their sensing activity, morphology, structure, and functional group contributions. Overall, this comprehensive review sheds light on the immense potential of biomass-derived carbon-based electrodes, encouraging further research to optimize their properties and advance their integration into practical electrochemical sensing devices.

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Sweetcorn husk derived porous carbon with inherent silica for ultrasensitive detection of ovarian cancer in blood plasma

Cited by 12 publications

References 42 publications

ZnO Nanoflower-Based Electrochemical SARS-CoV-2 Molecular Biosensors with Improved Diagnostic Accuracy

ZnO Nanoflower-Based Electrochemical SARS-CoV-2 Molecular Biosensors with Improved Diagnostic Accuracy

2D vanadium disulfide nanosheets assisted ultrasensitive, rapid, and label-free electrochemical quantification of cancer biomarker (MMP-2)

Biomass-Derived Carbon-Based Electrodes for Electrochemical Sensing: A Review

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