Swertisin rich fraction from Enicostema littorale ameliorates hyperglycemia and hyperlipidemia in high-fat fed diet and low dose streptozotacin induced type 2 diabetes mellitus in rats

Mokashi, Priyanka; Bhatt, Lokesh Kumar; Khanna, Aparna; Pandita, Nancy

doi:10.1016/j.biopha.2017.09.153

Cited by 15 publications

(11 citation statements)

References 25 publications

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“…Likewise, a cocoa flavonoid extract (1 µg/mL) and its main flavonoid epicatechin (EC) (10 µM) were able to diminish the insulin desensitization in glucose-induced insulin-resistant HepG2 cells by reducing IRS-1 serine phosphorylation and increasing tyrosine phosphorylated lev-els of IR, IRS-1 and IRS-2, and stimulating both the PI3K/AKT pathway and AMP-activated protein kinase (AMPK) [34]. Similar results were found with a swertisin rich flavonoid fraction both in insulin resistance hepatic cells [35] and in high fat diet (HFD)-fed and streptozotocin (STZ)-induced type 2 diabetic rats [36], as well as with the flavonoid tangeretin in (db/db) diabetic mice [37]. The hepatic insulin sensitizing effect of tangeretin [37] and cocoa flavonoids [55] has also been associated with their ability to suppress the MAPKs pathway.…”

Section: Effects Of Flavonoids On Insulin Signaling In T2dsupporting

confidence: 59%

Dietary Flavonoids and Insulin Signaling in Diabetes and Obesity

Ramos

2021

Cells

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Type 2 diabetes (T2D) and obesity are relevant worldwide chronic diseases. A common complication in both pathologies is the dysregulation of the insulin-signaling pathway that is crucial to maintain an accurate glucose homeostasis. Flavonoids are naturally occurring phenolic compounds abundant in fruits, vegetables and seeds. Rising evidence supports a role for the flavonoids against T2D and obesity, and at present, these compounds are considered as important potential chemopreventive agents. This review summarizes in vitro and in vivo studies providing data related to the effects of flavonoids and flavonoid-rich foods on the modulation of the insulin route during T2D and obesity. Notably, few human studies have evaluated the regulatory effect of these phenolic compounds at molecular level on the insulin pathway. In this context, it is also important to note that the mechanism of action for the flavonoids is not fully characterized and that a proper dosage to obtain a beneficial effect on health has not been defined yet. Further investigations will contribute to solve all these critical challenges and will enable the use of flavonoids to prevent, delay or support the treatment of T2D and obesity.

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Section: Effects Of Flavonoids On Insulin Signaling In T2dsupporting

confidence: 59%

Dietary Flavonoids and Insulin Signaling in Diabetes and Obesity

Ramos

2021

Cells

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“…After weighing, the empty sac was filled with 1 ml of Krebs–Henseleit bicarbonate buffer (KHB)of pH 7.4. After weighing, the distended sac was placed inside the volumetric flask containing 50 ml of KHB buffer with a solution of glucose maintained at 37°C, and tyrosine was incubated in the mucosal solution in the flask (Mokashi et al, ).…”

Section: Methodsmentioning

confidence: 99%

“…The everted sac technique was used to evaluate the rate of glucose absorption across an everted sac prepared from rat intestine (Mokashi, Bhatt, Khanna, & Pandita, 2017). The effect of LA (500 µg/ml) and BCP (500 µg/ml) on the absorption of glucose was studied in comparison with a control group.…”

Section: Evaluation Of Intestinal Glucose Absorption Rate (Everted mentioning

confidence: 99%

Insulinotropic and antidiabetic effects of β‐caryophyllene with l ‐arginine in type 2 diabetic rats

Kumawat

Kaur

2020

J Food Biochem

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Beta‐caryophyllene (BCP) is a flavoring agent, whereas l‐arginine (LA) is used as a food supplement. They possess insulinotropic and β cell regeneration activities, respectively. We assessed the antidiabetic potential of BCP, LA, and its combination in RIN‐5F cell lines and diabetic rats. Ex vivo studies were carried out for glucose uptake and absorption of the combination of BCP with LA. The results indicated that the combination of BCP with LA showed a significant decrease in glucose absorption and an increase in its uptake in tissues and also an increase in insulin secretion in RIN‐5F cells. The combination treatment of BCP with LA showed a significant reduction in glucose, lipid levels, and oxidative stress in pancreatic tissue when compared with the diabetic group. Furthermore, the combination of BCP with LA normalized glucose tolerance and pancreatic cell damage in diabetic rats. In conclusion, the combinational treatment showed significant potentials in the treatment of type 2 diabetes mellitus. Practical applications Type 2 diabetes mellitus is the most prevalent chronic metabolic disorder affecting a large population. Beta‐caryophyllene is a CB2 receptor agonist shown to have insulinotropic activity. l‐Arginine is a food supplement that possesses beta‐cell regeneration property. The combination of BCP with LA could work as a potential therapeutic intervention, considering the individual pharmacological activities of each. We evaluated the antidiabetic activity of the combination of BCP with LA in diabetic rats using ex vivo and in vitro experimentations. Results from the study revealed that the combination of BCP with LA showed a significant (p < .001) reduction in glucose and lipid levels as compared to individual treatment. In vitro study also supports the diabetic potential of the combination of BCP with LA in the glucose‐induced insulin secretion in RIN‐5F cell lines. The study indicates a therapeutic approach to treat T2DM by BCP and LA combination as food and dietary supplement.

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“…Over the past decades, the incidence of type 2 diabetes mellitus (T2DM) has significantly increased in the world, especially in developing country 2. The risk factors of T2DM include family history, lack of exercise, unhealthy diet, overweight, and obesity 3. In addition, high-fat diet (HFD) is associated with the disruption of intestinal barrier, immune response, and/or insulin resistance state, resulting in the increasing risk of T2DM 4.…”

Section: Introductionmentioning

confidence: 99%

<p>Silibinin attenuates high-fat diet-induced renal fibrosis of diabetic nephropathy</p>

K¹,

Zhou²,

Liu³

et al. 2019

DDDT

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Diabetic nephropathy (DN) is the leading cause of end-stage renal disease. Silibinin is a flavonoid compound which has medicinal value. Previous studies revealed that silibinin exhibited an anti-fibrotic effect. However, whether silibinin could attenuate high-fat diet (HFD)-induced renal fibrosis remains unclear. Therefore, this study aimed to explore the molecular mechanism by which silibinin regulated renal fibrosis induced by HFD. Methods: In the present study, human renal glomerular endothelial cells (HRGECs) were treated with various concentrations of silibinin. Then, cell viability and apoptosis were measured by MTT assay and flow cytometry, respectively. In addition, HRGECs were exposed to 100 nM TGF-β1 for mimicking in vitro renal fibrosis. The expressions of collagen I, fibronectin, and α-SMA were detected by reverse transcription-quantitative polymerasechain reaction and Western blot. Protein levels of p-IκB and p-p65 were examined by Western blot; meanwhile, level of NF-κB was measured by immunofluorescence staining. Furthermore, HFD-induced mouse model of renal fibrosis was established. The mouse body weight, fasting glucose, kidney weight/body weight, microalbuminuria, kidney histopathology, and fibrotic area were measured to assess the severity of renal fibrosis. Results: Low concentration of silibinin (≤50 μM) had no cytotoxicity, while high concentration of silibinin (≥75 μM) exhibited significant cytotoxicity. Additionally, TGF-β1 increased the expressions of collagen I, fibronectin, α-SMA, p-IκB, and p-p65 and decreased the level NF-κB, while these effects were notably reversed by 50 μM silibinin. Moreover, both 50 and 100 mg/kg silibinin greatly decreased HFD-induced the upregulation of kidney weight/body weight, microalbuminuria, and fibrotic area. 100 mg/kg silibinin markedly reduced collagen I, fibronectin, and p-p65 expressions in mice renal tissues. Conclusion: Silibinin was able to attenuate renal fibrosis in vitro and in vivo via inhibition of NF-κB. These data suggested that silibinin may serve as a potential agent to alleviate the renal fibrosis of DN.

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Swertisin rich fraction from Enicostema littorale ameliorates hyperglycemia and hyperlipidemia in high-fat fed diet and low dose streptozotacin induced type 2 diabetes mellitus in rats

Cited by 15 publications

References 25 publications

Dietary Flavonoids and Insulin Signaling in Diabetes and Obesity

Dietary Flavonoids and Insulin Signaling in Diabetes and Obesity

Insulinotropic and antidiabetic effects of β‐caryophyllene with l ‐arginine in type 2 diabetic rats

<p>Silibinin attenuates high-fat diet-induced renal fibrosis of diabetic nephropathy</p>

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