SWI/SNF Chromatin Remodelers: Structural, Functional and Mechanistic Implications

Singh, Abhilasha; Modak, Sharmila Basu; Chaturvedi, Madan M.; Purohit, Jogeswar S.

doi:10.1007/s12013-023-01140-5

Cited by 14 publications

(3 citation statements)

References 111 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Collectively, the RIME detected seven putative subunits of the SWI/SNF complex, including PbARID and gSNF2 (Figure 7C ). These includes six of seven subunits that are conserved among the SWI/SNF complexes of yeasts and humans, except for SNF5 orthologs (Figure 7D ) ( 58 , 75 ). SNF5 is a component of the arm submodule in the SWI/SNF complex ( 76 , 77 ).…”

Section: Resultsmentioning

confidence: 99%

PbARID-associated chromatin remodeling events are essential for gametocyte development in Plasmodium

Nishi,

Kaneko,

Iwanaga

et al. 2024

Nucleic Acids Research

View full text Add to dashboard Cite

Gametocyte development of the Plasmodium parasite is a key step for transmission of the parasite. Male and female gametocytes are produced from a subpopulation of asexual blood-stage parasites, but the mechanisms that regulate the differentiation of sexual stages are still under investigation. In this study, we investigated the role of PbARID, a putative subunit of a SWI/SNF chromatin remodeling complex, in transcriptional regulation during the gametocyte development of P. berghei. PbARID expression starts in early gametocytes before the manifestation of male and female-specific features, and disruption of its gene results in the complete loss of gametocytes with detectable male features and the production of abnormal female gametocytes. ChIP-seq analysis of PbARID showed that it forms a complex with gSNF2, an ATPase subunit of the SWI/SNF chromatin remodeling complex, associating with the male cis-regulatory element, TGTCT. Further ChIP-seq of PbARID in gsnf2-knockout parasites revealed an association of PbARID with another cis-regulatory element, TGCACA. RIME and DNA-binding assays suggested that HDP1 is the transcription factor that recruits PbARID to the TGCACA motif. Our results indicated that PbARID could function in two chromatin remodeling events and paly essential roles in both male and female gametocyte development.

show abstract

Section: Resultsmentioning

confidence: 99%

PbARID-associated chromatin remodeling events are essential for gametocyte development in Plasmodium

Nishi,

Kaneko,

Iwanaga

et al. 2024

Nucleic Acids Research

View full text Add to dashboard Cite

show abstract

“…Further, several ORF1p interacting proteins were constituents of the mating-type switching/sucrose nonfermenting complex (SWI/SNF complex), i.e. ARID1A, ARID1B, SMARCA2, SMARCB1, SMARCC2), an ATP-dependent chromatin remodeler complex disrupting nucleosome/DNA contacts to facilitate DNA/chromatin accessibility by shifting, removing or exchanging nucleosomes along DNA 56,57 . Finally, we also observed proteins belonging to the GO term "neuronal cell body", corroborating with the neuron-specific presence of ORF1p in the brain.…”

Section: Endogenous Orf1p Interactors In the Mouse Brainmentioning

confidence: 99%

Steady-state neuron-predominant LINE-1 encoded ORF1p protein and LINE-1 RNA increase with aging in the mouse and human brain

Bonnifet,

Sinnassamy,

Massiani-Beaudoin

et al. 2023

Preprint

View full text Add to dashboard Cite

Recent studies have established a reciprocal causal link between aging and the activation of transposable elements, characterized in particular by a de-repression of LINE-1 retrotransposons. These LINE-1 elements represent 21% of the human genome, but only a minority of these sequences retain the coding potential essential for their mobility. LINE-1 encoded proteins can induce cell toxicity implicated in aging and neurodegenerative diseases. However, our knowledge of the expression and localization of LINE-1-encoded proteins in the central nervous system is limited. Using a novel approach combining atlas-based brain mapping with deep-learning algorithms on large-scale pyramidal brain images, we unveil a heterogeneous, neuron-predominant and widespread ORF1p expression throughout the murine brain at steady-state. In aged mice, ORF1p expression increases significantly which is corroborated in human post-mortem dopaminergic neurons by an increase in young LINE-1 elements including those with open reading frames. Mass spectrometry analysis of endogenous mouse ORF1p revealed novel, neuron-specific protein interactors. These findings contribute to a comprehensive description of the dynamics of LINE-1 and ORF1p expression in the brain at steady-state and in aging and provide insights on ORF1p protein interactions in the brain.

show abstract

“…The complexes contain 10–12 subunits, some of which are common, while others are specific to each of the complexes. A number of subunits of the complexes have domains that recognize various modifications of the N-terminal histone sequences, which determines the interaction of the complex with a certain chromatin region [ 4 ].…”

mentioning

confidence: 99%

Neuronal and Muscle Differentiation of Mammalian Cells Is Accompanied by a Change in PHF10 Isoform Expression

Bayramova,

Azieva,

Feoktistov

et al. 2024

Dokl Biochem Biophys

View full text Add to dashboard Cite

The PBAF chromatin remodeling complex of the SWI/SNF family plays a critical role in the regulation of gene expression during tissue differentiation and organism development. The subunits of the PBAF complex have domains responsible for binding to N-terminal histone sequences. It determines the specificity of binding of the complex to chromatin. PHF10, a specific subunit of the PBAF complex, contains a DPF domain, which is a unique chromatin interaction domain. A PHF10 isoform that lacks the DPF domain is also present in vertebrate cells. This work shows that during neuronal and muscle differentiation of human and mouse cells, the expression of PHF10 isoforms changes: the form that does not have DPF replaces the form in which it is present. Replacement of PHF10 isoforms in the PBAF complex may affect its selectivity in the regulation of genes in differentiating cells.

show abstract

SWI/SNF Chromatin Remodelers: Structural, Functional and Mechanistic Implications

Cited by 14 publications

References 111 publications

PbARID-associated chromatin remodeling events are essential for gametocyte development in Plasmodium

PbARID-associated chromatin remodeling events are essential for gametocyte development in Plasmodium

Steady-state neuron-predominant LINE-1 encoded ORF1p protein and LINE-1 RNA increase with aging in the mouse and human brain

Neuronal and Muscle Differentiation of Mammalian Cells Is Accompanied by a Change in PHF10 Isoform Expression

Contact Info

Product

Resources

About