Swi5 Controls a Novel Wave of Cyclin Synthesis in Late Mitosis

Aerne, Birgit L.; Johnson, Anthony L.; Toyn, Jeremy H.; Johnston, Leland H.

doi:10.1091/mbc.9.4.945

Cited by 55 publications

(54 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Swi5p is expressed in S phase, in which it remains localized in the cytoplasm until it enters the nucleus in late anaphase after inactivation of a Clbp/Cdc28p kinase (Moll et al 1991;Tebb et al 1993). After its arrival in the nucleus, Swi5p is competent for DNA binding and transcriptional activation, because SWI5 is required for expression of a number of genes in early G 1 , such as ASH1 (Bobola et al 1996), SIC1 (Knapp et al 1996;Toyn et al 1997), EGT2 (Kovacech et al 1996), CDC6 (Piatti et al 1995), PCL2 and PCL9 (Aerne et al 1998). Although Swi5p probably binds in early G 1 to its two sites far upstream of the HO TATA element (see Fig.…”

Section: Discussionmentioning

confidence: 99%

Cell cycle-regulated histone acetylation required for expression of the yeast HO gene

Krebs¹,

Kuo²,

Allis³

et al. 1999

Genes & Development

266

207

View full text Add to dashboard Cite

Expression of the yeast HO gene in late G 1 of the cell cycle requires the SWI/SNF chromatin remodeling complex, the Gcn5p histone acetyltransferase, and two different sequence-specific transcriptional activators, Swi5p and Swi4p/Swi6p. We have used chromatin immunoprecipitation assays to investigate the role of each of these trans-acting factors in establishing a cell cycle-regulated domain of histone acetylation surrounding the HO upstream regulatory region. We detect a ∼1-kb domain of H3 and H4 acetylation that is established in mid-G 1 , prior to and independent of HO transcription, which then declines with kinetics similar to inactivation of HO. This cell cycle burst of histone acetylation requires Gcn5p, SWI/SNF, and the Swi5p activator, but occurs in the absence of the Swi4p activator. We also find that inactivation of the Sin3p/Rpd3p deacetylase complex leads to a high level of acetylation at the HO locus throughout the cell cycle. We propose a sequential model for activation of HO in which the Swi5p-dependent recruitment of the Gcn5p acetyltransferase requires chromatin remodeling events by the SWI/SNF complex.

show abstract

Section: Discussionmentioning

confidence: 99%

Cell cycle-regulated histone acetylation required for expression of the yeast HO gene

Krebs¹,

Kuo²,

Allis³

et al. 1999

Genes & Development

266

207

View full text Add to dashboard Cite

show abstract

“…These functions appear to be exerted through the binding of different cyclins (Huang et al 1998). Some cyclins are synthesized periodically while the others are constitutively present (Measday et al 1997;Aerne et al 1998). Thus it is reasonable to assume that there are mechanisms which distinguish cyclins for Pho85p to carry out different functions in response to various signals, which are either related or unrelated to the cell cycle progression.…”

Section: Distinct Regulation Mechanism and Pleiotropic Function Of Phmentioning

confidence: 99%

The Pho85 kinase, a member of the yeast cyclin‐ dependent kinase (Cdk) family, has a regulation mechanism different from Cdks functioning throughout the cell cycle

et al. 1999

View full text Add to dashboard Cite

Background: The PHO85 gene is a negative regulator of the PHO system in the yeast Saccharomyces cerevisiae and encodes a protein kinase (Pho85p) which is highly homologous to the Cdc28 kinase (Cdc28p). Although the two kinases share a 51% identity and their functional domains are well conserved, PHO85 fails to replace CDC28. Pho85p forms complexes with G 1 -cyclin homologues, including Pcl1p, Pcl2p and Pcl9p, and is thought to be involved in the cell-cycle regulation at G 1 and the end of M. By analysing the genetic and biochemical properties of Pho85p, we studied whether the regulation of Pho85p activity is similar to other cyclin-dependent kinases (Cdks) directly involved in cell cycle regulation.

show abstract

“…This transcription factor is very similar to Swi5p but, in spite of their similar DNA binding sequences, each of them regulates the expression of specific target genes. For example, Swi5p controls the expression of the genes encoding HO endonuclease (Dohrmann et al, 1992), cyclins Pcl2p and Pcl9p (Aerne et al, 1998) and the cell wall protein Pir1p (Doolin et al, 2001), whereas Ace2p regulates the expression of CTS1, ENG1, SCW11, DSE1 and DSE2 (Baladrón et al, 2002;Dohrmann et al, 1992;Doolin et al, 2001). There is also a third group of genes, whose expression is dependent on both transcription factors, including EGT2, SIC1, ASH1 and RME1 (Doolin et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Swm1p subunit of the APC/cyclosome is required for activation of the daughter-specific gene expression program mediated by Ace2p during growth at high temperature inSaccharomyces cerevisiae

Ufano

Pablo

Calzada

et al. 2004

Journal of Cell Science

View full text Add to dashboard Cite

SWM1 was originally identified for its role in the late steps of the sporulation process, being required for spore wall assembly. This protein, recently identified as one of the core subunits of the anaphase-promoting complex (APC) is also required to complete cell separation in vegetative cells during growth at high temperature. Mutants lacking SWM1 show a thermosensitive growth defect that is suppressed by osmotic support in the culture medium. At the restrictive temperature, swm1 mutants are unable to complete separation, forming chains of cells that remain associated and, with prolonged incubation times, the stability of the cell wall is compromised, resulting in cell lysis. This separation defect is due to a reduction in expression of CTS1 (the gene encoding chitinase) and a group of genes involved in cell separation (such as ENG1, SCW11, DSE1 and DSE2). Interestingly, these genes are specifically regulated by the transcription factor Ace2p, suggesting that Swm1p is required for normal expression of Ace2p-dependent genes during growth at high temperatures. Although no defect in Ace2p localization can be observed at 28°C, this transcription factor is unable to enter the nucleus of the daughter cell during growth at 38°C. Under these growth conditions, swm1 cells undergo a delay in exit from mitosis, as determined by analysis of Clb2p degradation and Cdc28p-Clb2p kinase assays, and this could be the reason for the cytoplasmic localization of Ace2p.

show abstract

Swi5 Controls a Novel Wave of Cyclin Synthesis in Late Mitosis

Cited by 55 publications

References 47 publications

Cell cycle-regulated histone acetylation required for expression of the yeast HO gene

Cell cycle-regulated histone acetylation required for expression of the yeast HO gene

The Pho85 kinase, a member of the yeast cyclin‐ dependent kinase (Cdk) family, has a regulation mechanism different from Cdks functioning throughout the cell cycle

Swm1p subunit of the APC/cyclosome is required for activation of the daughter-specific gene expression program mediated by Ace2p during growth at high temperature inSaccharomyces cerevisiae

Contact Info

Product

Resources

About